BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0068.p (517 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313026|ref|YP_003777118.1| signal transduction protein con... 863 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 106 5e-24 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 102 1e-22 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 101 1e-22 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 100 2e-22 >gi|300313026|ref|YP_003777118.1| signal transduction protein containing sensor and EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 863 bits (2230), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/427 (99%), Positives = 427/427 (100%) Query: 1 VSNKNRIVAWTIVATAVAAIAPVAAMMWLSRQAVAQAEHERLARYAESVIARAAHSFDET 60 +SNKNRIVAWTIVATAVAAIAPVAAMMWLSRQAVAQAEHERLARYAESVIARAAHSFDET Sbjct: 1 MSNKNRIVAWTIVATAVAAIAPVAAMMWLSRQAVAQAEHERLARYAESVIARAAHSFDET 60 Query: 61 QRSLKSLSPLLLLPCSDEHIAQMRLISVNSRSIEEMGYYEHGQLKCTSWDSSVGARRRRQ 120 QRSLKSLSPLLLLPCSDEHIAQMRLISVNSRSIEEMGYYEHGQLKCTSWDSSVGARRRRQ Sbjct: 61 QRSLKSLSPLLLLPCSDEHIAQMRLISVNSRSIEEMGYYEHGQLKCTSWDSSVGARRRRQ 120 Query: 121 AEPVFTTADGVEVALDQRSFVADGPTMLSIQYGAHSALVHRLRFVDVVLEKGVHVVLARN 180 AEPVFTTADGVEVALDQRSFVADGPTMLSIQYGAHSALVHRLRFVDVVLEKGVHVVLARN Sbjct: 121 AEPVFTTADGVEVALDQRSFVADGPTMLSIQYGAHSALVHRLRFVDVVLEKGVHVVLARN 180 Query: 181 GFGPVAQYGGPVAAGVAEHALDGLGRQDVDGFFISTVSRDGWTAAAIVDGDHVLEEMTGQ 240 GFGPVAQYGGPVAAGVAEHALDGLGRQDVDGFFISTVSRDGWTAAAIVDGDHVLEEMTGQ Sbjct: 181 GFGPVAQYGGPVAAGVAEHALDGLGRQDVDGFFISTVSRDGWTAAAIVDGDHVLEEMTGQ 240 Query: 241 QRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGA 300 QRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGA Sbjct: 241 QRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGA 300 Query: 301 EALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHIAINV 360 EALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHIAINV Sbjct: 301 EALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHIAINV 360 Query: 361 AAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHSMALD 420 AAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHSMALD Sbjct: 361 AAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHSMALD 420 Query: 421 DFGTGYS 427 DFGTGYS Sbjct: 421 DFGTGYS 427 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 106 bits (264), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 61/164 (37%), Positives = 96/164 (58%), Gaps = 2/164 (1%) Query: 266 RLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEE 325 RL DL+ A+ EF++HYQP +++ASGR IG+EAL+RW+ P +V P FI LAEE Sbjct: 691 RLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEE 750 Query: 326 SGLILPLTDLVVDTVVREMRD-TLRQDRALHIAINVAAQDMKTGRILEVVSQALAGSDIR 384 +GLILP+ V+ + R L + +++N++ ++ ++ L + + Sbjct: 751 TGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLP 810 Query: 385 PEQLWMEATERGCM-DIASASLTLARAREMGHSMALDDFGTGYS 427 PE L +E TE M D+ A LA +++G +++DDFGTGYS Sbjct: 811 PESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYS 854 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 102 bits (253), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 61/156 (39%), Positives = 90/156 (57%), Gaps = 2/156 (1%) Query: 274 QLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLT 333 ++A+++ +F + YQP + +G +GAEALLRW P +V PD FI LAE SGLILP+ Sbjct: 445 RVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERSGLILPIG 504 Query: 334 DLVVDTVVREMRDTLRQDRAL-HIAINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEA 392 V+D R+MR+ +++N++A +LEVV L + P L +E Sbjct: 505 AWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEV 564 Query: 393 TERGCMDIASASL-TLARAREMGHSMALDDFGTGYS 427 TE M ASL TL + ++G +++DDFGTGYS Sbjct: 565 TESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYS 600 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 55/153 (35%), Positives = 88/153 (57%), Gaps = 1/153 (0%) Query: 276 AVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLTDL 335 A+++ EF+++ QP +E+ SG+ +GAEAL+RW P+ + PD FI AE +G I LT Sbjct: 541 ALERDEFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLW 600 Query: 336 VVDTVVREMRDTLRQDRALHIAINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEATER 395 +++ V R +Q L ++N++ +D+ + + L ++P L +E TE Sbjct: 601 MMEQVARLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITES 660 Query: 396 GCMDI-ASASLTLARAREMGHSMALDDFGTGYS 427 MD A A TL R MG ++++DDFGTGYS Sbjct: 661 AIMDDPARAQNTLERLSNMGFALSIDDFGTGYS 693 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 60/165 (36%), Positives = 95/165 (57%), Gaps = 4/165 (2%) Query: 266 RLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEE 325 R++ A+L+ A+++ EF+VHYQP IE+A GR +GAEAL+RW+ P ++ P FI LAEE Sbjct: 842 RIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEE 901 Query: 326 SGLILPLTDLVVDTVVREMRDTLRQDRAL--HIAINVAAQDMKTGRILEVVSQALAGSDI 383 + LI + LV+D R++ +RQ + I+IN+ R+L+ + + + Sbjct: 902 TRLIDQIGLLVLDVACRDI-GLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGT 960 Query: 384 RPEQLWMEATERGCMDIASASLTLARA-REMGHSMALDDFGTGYS 427 P L E TE M ++ + R G ++++DDFGTGYS Sbjct: 961 PPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYS 1005 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,274,583 Number of extensions: 46524 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 17 Length of query: 517 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 428 Effective length of database: 1,198,455 Effective search space: 512938740 Effective search space used: 512938740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)