BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0067.p (454 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 942 0.0 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 145 1e-35 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 137 1e-33 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 122 4e-29 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 110 2e-25 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 942 bits (2436), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/454 (99%), Positives = 454/454 (100%) Query: 1 MGHCDVFEVSQVCPLPAAKEEAPDFCALIKANARYRHIIGNTPAIIYSSVPSGDFRLTFV 60 MGHCDVFEVSQVCPLPAAKEEAPDFCALIKANARYRHIIGNTPAIIYSSVPSGDFRLTFV Sbjct: 1 MGHCDVFEVSQVCPLPAAKEEAPDFCALIKANARYRHIIGNTPAIIYSSVPSGDFRLTFV 60 Query: 61 SENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIYEYRFRTSNGSY 120 SENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIYEYRFRTSNGSY Sbjct: 61 SENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIYEYRFRTSNGSY 120 Query: 121 LWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQAKEQRKLIEQLKMAQAQLMQ 180 LWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQA+EQRKLIEQLKMAQAQLMQ Sbjct: 121 LWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQ 180 Query: 181 SEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQ 240 SEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQ Sbjct: 181 SEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQ 240 Query: 241 EQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGL 300 EQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGL Sbjct: 241 EQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGL 300 Query: 301 DSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTA 360 DSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTA Sbjct: 301 DSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTA 360 Query: 361 RAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVS 420 RAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVS Sbjct: 361 RAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVS 420 Query: 421 SAPGCGTTFTIELPIDPASVLAQSSATETSGEAS 454 SAPGCGTTFTIELPIDPASVLAQSSATETSGEAS Sbjct: 421 SAPGCGTTFTIELPIDPASVLAQSSATETSGEAS 454 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 145 bits (365), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 110/379 (29%), Positives = 168/379 (44%), Gaps = 68/379 (17%) Query: 86 IHPDD----TAQIFSSLAMLFVEDQKIYEYRFRTSNGSYLWMHDTLRLIRDPDGTPQEVI 141 +HPDD +A+I +LA ++YR R +G Y W+ R+ PDG P + Sbjct: 197 VHPDDRELLSARIREALAR---GGAYAHQYRTRRRDGRYYWLEANGRVEMTPDGIPSKFP 253 Query: 142 GLMTDITGRKEMEDTLQRQAKEQRKLIEQLKM-----------AQAQLMQSEKLASIGQL 190 G++ DI GR+ +E R R L E L+ A+ L Q++K+ ++GQL Sbjct: 254 GVLIDIEGRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVGQL 313 Query: 191 AAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQEQLRSFEARA 250 G+AH+ NN + +S +L L ++L Q G Sbjct: 314 TGGLAHDFNNILAGISGSLE---------LMKVRLAQGRIG------------------- 345 Query: 251 DLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNE 310 D+ L + ++R + Q L FSR + + DL+R + + Sbjct: 346 -------DIERHLNGAQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRS 398 Query: 311 LKYKVSI-VKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTAR-------- 361 + +++ G L ++ +NL +NA A+ G +TI TA Sbjct: 399 VGPAIAVETIAAGGLWHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIGA 458 Query: 362 ------AGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGG 415 G VS+S+SDTG G++PE + R F+PFFTTKP G GTGLGLS+ YG + GG Sbjct: 459 LQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGG 518 Query: 416 DIRVSSAPGCGTTFTIELP 434 +R+ S G G I LP Sbjct: 519 TVRIYSELGQGAMVCIYLP 537 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 137 bits (346), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 119/386 (30%), Positives = 182/386 (47%), Gaps = 32/386 (8%) Query: 56 RLTFVSENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIFSSLAMLFVEDQKIY--EYRF 113 R V++ R+LG EML + HPDD + SL +ED + + E R+ Sbjct: 592 RYLRVNQALCRMLGRSEEEMLG--MTLADVTHPDDVP-MNMSLFNRCIEDGRSFTLEKRY 648 Query: 114 RTSNGSYLWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQR--QAKEQRKLIEQL 171 +GS W+ + I D +G PQ +I + TDIT R+ E+ L+ EQR + E L Sbjct: 649 LRPDGSTFWVSSDISRIVDGEGRPQAMIAVSTDITERRRAEEALRELNDTLEQRVVEEIL 708 Query: 172 KM--AQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCL 229 + A+A L Q +K+ ++GQL GVAH+ NN + + NL L + Q + Sbjct: 709 ERSRAEAALRQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQSDPRLDERSRQRVRIA 768 Query: 230 QGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGES 289 G A QL +F R L S ++ ++ ++ L+R + +++ +R+ Sbjct: 769 IGAVDRGAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRA--VGENIDISTRVAHD 826 Query: 290 NWQ-MTDLHRGLDSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAAQA 348 W M D ++ + +N A N +P L +++ V + V +QA Sbjct: 827 LWNTMLDPNQVENLIINTAINA----------RDAMPSGGRLTVQIDNVRLEDPVFLSQA 876 Query: 349 ISERGTVTICTARAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYG 408 G+ V + ISD G G++PE +R FEPFFTTK G GTGLGLS++YG Sbjct: 877 ----------DLAPGEYVELQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYG 926 Query: 409 IVKKHGGDIRVSSAPGCGTTFTIELP 434 VK+ G I + S G GTT I P Sbjct: 927 FVKQSKGHISIHSEQGKGTTIRIYFP 952 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 122 bits (307), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 109/400 (27%), Positives = 178/400 (44%), Gaps = 79/400 (19%) Query: 57 LTFVSENASRVLGYEPCEMLDDPNFWFNHIHPDDTAQIF----SSLAM---LFVED---- 105 L + S+ R+ G P + +HPDD ++ +++AM + VE Sbjct: 185 LMYCSDELCRIYGLAPGRNCITYEEFAAIMHPDDRQEVVETVNAAVAMGGTIRVEHRICR 244 Query: 106 QKIYEYRFRTSNGSYLWMHDTLRLIRDPDGTPQEVIGLMTDITGRKEMEDTLQRQAKEQR 165 Q E R+ + G +W DGT E +G TDIT R++ E + Sbjct: 245 QDTGEVRYLSGIGKPIWR----------DGTFTEYVGTATDITVRRQNEYAI-------- 286 Query: 166 KLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQL 225 + AQ + + + ++GQL A +AHEIN P+ + AN Sbjct: 287 ------RAAQIDMERVSRANTVGQLTASIAHEINQPLMSIVAN----------------- 323 Query: 226 HQCLQGGALPAAMAQEQLRSFEARA-DLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFS 284 A LR + DL + +++AE QR I+ L+D + Sbjct: 324 -------------AGASLRWLDRPVPDLEHARTGLRDIIAEG----QRAGGIISGLRDLT 366 Query: 285 RIGESNWQMTDLHRGLDSTLNIANNELKYK-VSIVKQYGEL-PLVKCLASELNQVFMNLL 342 R + +LH + L ++ +EL+ + VS++ V +L QV +NL+ Sbjct: 367 RNAAPVCERVNLHAVIRHILTLSRSELERREVSLLLSLTPADAFVLGDQVQLQQVMLNLV 426 Query: 343 VNAAQAIS---ERGTVTICTARAGD--KVSVSISDTGHGIAPENLNRIFEPFFTTKPVGS 397 VNA +A+S E V TA + D ++ V + D+G GIAPE + R+F+ F+TTK S Sbjct: 427 VNAIEAMSSMPEHARVLSITASSCDEQRIEVKVQDSGEGIAPEAMRRVFDAFYTTK--DS 484 Query: 398 GTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPIDP 437 G G+GL++ I++ H G +RV++A G F+ +LP P Sbjct: 485 GMGMGLAICRSIIENHRGRLRVAAAQPHGALFSFDLPRVP 524 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 91/308 (29%), Positives = 142/308 (46%), Gaps = 62/308 (20%) Query: 170 QLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCL 229 +L + L S+K+ +IGQL G+AH+ NN +G +S +L+ + L++G Sbjct: 451 ELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLM---RVRLVQG------- 500 Query: 230 QGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSR---I 286 +E LR EL A S QR ++ Q L F+R + Sbjct: 501 --------RGEELLR--------------YVELAARST---QRAASLTQRLLAFARRQTL 535 Query: 287 GESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQYGELPLVKCLASELNQVFMNLLVNAA 346 ++ L G++ + + +V G P +L +NL +NA Sbjct: 536 APKPTRVNALISGMEELIRRTIGP-AIVLEVVGAGGLWPTF-VDGPQLESALLNLCINAR 593 Query: 347 QAISERGTVTICTARA--------------GDKVSVSISDTGHGIAPENLNRIFEPFFTT 392 A+ G +TI TA G V +S++D+G G+ P+ L R F+PFFTT Sbjct: 594 DAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMPPDVLARAFDPFFTT 653 Query: 393 KPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPI--------DPASVLAQS 444 KP+G+GTGLGLS+ YG ++ GG +R+ S G GTT +I LP D A L ++ Sbjct: 654 KPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLPRHQHDGDEQDEADALDET 713 Query: 445 SATETSGE 452 +A + +GE Sbjct: 714 AAGDGNGE 721 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,276,509 Number of extensions: 48354 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 31 Length of query: 454 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 366 Effective length of database: 1,203,190 Effective search space: 440367540 Effective search space used: 440367540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)