BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0057.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 590 e-170 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 128 6e-31 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 125 5e-30 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 125 5e-30 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 124 1e-29 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 590 bits (1520), Expect = e-170, Method: Compositional matrix adjust. Identities = 287/290 (98%), Positives = 289/290 (99%) Query: 1 MEDSMSKTEIDALPAQHQSHQPGSEQQMEPRPHSGPAGEPGRRLAGKVALITGGDSGIGR 60 MEDSMSKTEIDALPAQHQSHQPGSEQQMEPRPHS PAGEPGRRLAGKVALITGGDSGIGR Sbjct: 80 MEDSMSKTEIDALPAQHQSHQPGSEQQMEPRPHSAPAGEPGRRLAGKVALITGGDSGIGR 139 Query: 61 AVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPEFCRSAVRAVMD 120 AVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPEFCRSAVRAVMD Sbjct: 140 AVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPEFCRSAVRAVMD 199 Query: 121 AYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMPFLTRGARIINT 180 AYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMPFLTRGARIINT Sbjct: 200 AYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMPFLTRGARIINT 259 Query: 181 TSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLAAAGIHVNAVAPGPIWTPLIPASFSAE 240 TSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLA+AGIHVNAVAPGPIWTPLIPASFSAE Sbjct: 260 TSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVNAVAPGPIWTPLIPASFSAE 319 Query: 241 EVANFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT 290 EVA+FGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT Sbjct: 320 EVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVNT 369 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 128 bits (321), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 88/247 (35%), Positives = 135/247 (54%), Gaps = 4/247 (1%) Query: 44 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 103 LAGKV I+G +GIG A A A+A G+ L + Y E++A +V+A + + + Sbjct: 4 LAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGA 63 Query: 104 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 163 DV +AV + GR+DVL+NNA +E ++ + N S+ Sbjct: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAF 123 Query: 164 VKEAMPFLTR--GARIINTTSVTA-YRGSKHLVDYAATKGAIVAFTRSLSQQLAAAGIHV 220 + +P + G IIN TSV A + G + YAA+KG + TR ++++L A I V Sbjct: 124 TRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRV 183 Query: 221 NAVAPGPIWTPLIPASFSAEEVANFGKNVPLGRPGQPDEVAPAYVFLATEGAS-YMTGQV 279 NAVAPG I TP + E++ F K +P+GR G+PDE + A+++LA+E S Y+TGQ+ Sbjct: 184 NAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQI 243 Query: 280 LHPNGGE 286 + NGG+ Sbjct: 244 IDVNGGQ 250 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 125 bits (313), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 86/248 (34%), Positives = 124/248 (50%), Gaps = 6/248 (2%) Query: 44 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 103 +A KV L+TGG GIG A AL + G +A+ Y+ A +QA + + G Sbjct: 3 MADKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGG 62 Query: 104 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 163 DV E V GR+ LVNNA +E++S ++ N+ Sbjct: 63 DVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLC 122 Query: 164 VKEAMPFLT-----RGARIINTTSVTAYRGS-KHLVDYAATKGAIVAFTRSLSQQLAAAG 217 +EA+ ++ +G I+N +S+ A G VDYAA+KGAI AFT L++++AA G Sbjct: 123 AREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEG 182 Query: 218 IHVNAVAPGPIWTPLIPASFSAEEVANFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 277 I VNAV PG I+T + + A V VP+ R G EVA A ++L +E ASY TG Sbjct: 183 IRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATG 242 Query: 278 QVLHPNGG 285 + +GG Sbjct: 243 TFIDVSGG 250 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 125 bits (313), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 79/252 (31%), Positives = 134/252 (53%), Gaps = 11/252 (4%) Query: 44 LAGKVALITGGD--SGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLL 101 L KVA+ITGG +G+G A A A +GAR+ ++ L + + A + + + L L Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAAR----IGSQHLGL 58 Query: 102 PGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMF 161 DV C +A A++ +GR+D+LVNNA P +++E I+ A + ++ Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLE-ITGADYDRILDVSLRGTL 117 Query: 162 YMVKEAMPFL--TRGARIINTTSVTAYRGSKHLVD--YAATKGAIVAFTRSLSQQLAAAG 217 YM + +P + + II +SV+A RG L Y+A K ++ R+++++ G Sbjct: 118 YMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEG 177 Query: 218 IHVNAVAPGPIWTPLIPASFSAEEVANFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 277 I VN++ PG I T +I + E+ A + +PL R G+ D++ A VFL ++ + Y TG Sbjct: 178 IRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTG 237 Query: 278 QVLHPNGGEIVN 289 L NGG +++ Sbjct: 238 ITLDVNGGMLIH 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 86/252 (34%), Positives = 133/252 (52%), Gaps = 11/252 (4%) Query: 44 LAGKVALITGG--DSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLL 101 L KVA+ITGG +G+G + A A +GAR+ I+ L+ E A + L A L L Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAAR----LGAQHLGL 58 Query: 102 PGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMF 161 DV C +A AV+ +GR+D+LVNNA PA+ + EI+ + ++ Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAK-LLEITGKDYDRILDVSLRGTL 117 Query: 162 YMVKEAMPFLT--RGARIINTTSVTAYRGSKHLVD--YAATKGAIVAFTRSLSQQLAAAG 217 YM + +P + + I+ +SV+A RG L Y+A K ++ R+++++ G Sbjct: 118 YMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 218 IHVNAVAPGPIWTPLIPASFSAEEVANFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTG 277 I VN + PG I T + SAE+ A + +PLGR G PD+V VFLA++ + Y TG Sbjct: 178 IRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 278 QVLHPNGGEIVN 289 L NGG +++ Sbjct: 238 VTLDVNGGMLIH 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 959,062 Number of extensions: 42203 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 51 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)