BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0052.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313041|ref|YP_003777133.1| major facilitator superfamily p... 860 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 199 4e-52 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 189 6e-49 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 175 6e-45 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 169 6e-43 >gi|300313041|ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 436 Score = 860 bits (2222), Expect = 0.0, Method: Compositional matrix adjust. Identities = 434/436 (99%), Positives = 434/436 (99%) Query: 1 MTTSAEFTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 MTTSAEFTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM Sbjct: 1 MTTSAEFTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 Query: 61 QEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 EWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY Sbjct: 61 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 Query: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG Sbjct: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 Query: 181 SLMGIFINSAFEPGEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANE 240 SLMGIFINSAFEP EVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANE Sbjct: 181 SLMGIFINSAFEPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANE 240 Query: 241 LPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLM 300 LPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLM Sbjct: 241 LPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLM 300 Query: 301 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSI 360 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSI Sbjct: 301 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSI 360 Query: 361 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL Sbjct: 361 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 Query: 421 GVLLAFVPMSSKGYRA 436 GVLLAFVPMSSKGYRA Sbjct: 421 GVLLAFVPMSSKGYRA 436 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 199 bits (505), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 141/418 (33%), Positives = 217/418 (51%), Gaps = 36/418 (8%) Query: 12 NESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPA--DMQEWLRMLQT 69 N+ + R + ++ +G LE++DF +Y FFA ++ KLFFP D+ L + T Sbjct: 16 NQQRRRAIVAT--------VIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVAT 67 Query: 70 FGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPI 129 FG+ G+ RPVGGI++ Y D +GRK +++I LMAV T +IGL PTY SIG+ AP+ Sbjct: 68 FGV---GFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPL 124 Query: 130 LLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINS 189 ++++ R LQG + GGEM A F+ E+ P ++ + A I +LLG+ +G F+ + Sbjct: 125 IIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTA 184 Query: 190 AFEPGEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANELPLKTVLRE 249 + + +A + WR+PF+LG + G V Y+R +LEETP F E K+ + PLK V+R Sbjct: 185 SLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLE----EKDKTDSPLKEVIRN 240 Query: 250 HRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLMLTIGCLAIG 309 + + M + V++ P+Y V L A G L + + +G Sbjct: 241 FPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVG 300 Query: 310 WLCDRIGTRASMIIGWGGLGLSTWW-FYGQLPGSPE-------SLVWGYALVGLFVGSIS 361 L DRIG R ++ G L L + + + +P LV+G L+ + G Sbjct: 301 RLADRIGRR-PLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFG-TLIAAYTG--- 355 Query: 362 LTPIVS--TRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKA--DPQGPALYVI 415 PI++ + FP V TGLS AYN+A +FGG I+ I A PA YV+ Sbjct: 356 --PILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVM 411 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 189 bits (479), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 123/396 (31%), Positives = 195/396 (49%), Gaps = 7/396 (1%) Query: 32 LGGTLEFYDFVIYVFFAAVVGKLFFPADMQEWLRMLQTFGIFAAGYLARPVGGIIIAHYG 91 +G LE+YDF++Y FF+A++ +LFFPAD E+ +L F G+ RPVGG+++ Y Sbjct: 16 VGNALEWYDFIVYGFFSAIIARLFFPAD-NEYTSLLVALATFGIGFFMRPVGGVLLGLYA 74 Query: 92 DVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWV 151 D GRK + I LM + +I PTYA+IG+AAPI++++ R LQG A GGE + Sbjct: 75 DRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTA 134 Query: 152 FVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSAFEPGEVAGYAWRIPFILGGVF 211 F+ E PA++ G + G+ MG + P + + WR+PF+LG + Sbjct: 135 FLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLI 194 Query: 212 GLVSVYLRRYLEETPVFRELAARRKEANELPLKTVLREHRAASVLTAGMTWCLSGAVVVV 271 G V +++RR++ ET F E +A + A + L V++ + +++ G + A VV Sbjct: 195 GPVGLWIRRHMHETDDFLE-SANKPNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAFYVV 253 Query: 272 ILMTPSYLQTVHHLSPKLVMQANCAGTLMLTIGCLAIGWLCDRIGTRASMIIGWGGLGLS 331 ++ P++ L +LT G L DR+G R ++ L Sbjct: 254 LVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLYSSLAFLLM 313 Query: 332 TWWFY---GQLPGSPESLVWGYALVGLFVGSISLTPIVSTRAFPPPVRFTGLSFAYNMAY 388 + + P P LV L + + P + FP VR TGL+ AYN+A Sbjct: 314 VYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAYNLAA 373 Query: 389 AVFGGLTPVLISLWIKA--DPQGPALYVIGLSILGV 422 +FGG P +++ K P PA YV+ + +GV Sbjct: 374 MLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGV 409 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 175 bits (444), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 127/402 (31%), Positives = 197/402 (49%), Gaps = 14/402 (3%) Query: 30 SALGGTLEFYDFVIYVFFAAVVGKLFFPADMQEWLRMLQTFGIFAAGYLARPVGGIIIAH 89 S++G LE++D +IY FA V+ K FFP + + +L TF F + RP+G +++ Sbjct: 24 SSIGNALEWFDILIYGAFAVVIAKQFFPTG-DDSVSLLLTFATFGVSFFMRPLGAVVLGA 82 Query: 90 YGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGA 149 Y D GRK LSI LM V T +I +P+YASIG+ AP + L + +QG + GGE + Sbjct: 83 YSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSS 142 Query: 150 WVFVAEHVPANRYSFGVGSL-TAGITGGILLGSLMGIFINSAFEPGEVAGYAWRIPFILG 208 F+ EH P R F + + GI+ +LL +L G +N+ P ++A + WR+PFI G Sbjct: 143 TAFLVEHAPHRRGFFSSWQVASQGIS--LLLAALFGAVLNNMLTPEQLASWGWRVPFIFG 200 Query: 209 GVFGLVSVYLRRYLEETPVFRELAARRKEANELPLKTVLREHRAASVLTAGMTWCLSGAV 268 + +Y+RR+L+E P F+E E PL+ + ++ AG + ++ Sbjct: 201 LLIAPAGIYIRRHLDEAPEFKE----SSEKTNAPLRDTFAHQKMRLLIGAG-SVIMATVS 255 Query: 269 VVVILMTPSYLQTVHHLSPKLVMQANCAGTLMLTIGCLAIGWLCDRIGTRASMIIG---W 325 V + L P+Y L A L++ IG +G L D+IG MI + Sbjct: 256 VYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALY 315 Query: 326 GGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSISLTPIVSTRAFPPPVRFTGLSFAYN 385 L + F PG + L+ + L + P + FP R TG+S AYN Sbjct: 316 IVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYN 375 Query: 386 MAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFV 427 +A +FGG ++I+ I D G L V I G +L+ + Sbjct: 376 IAVTIFGGFAGLIITWLI--DFTGDKLSVSYFVIFGAVLSLI 415 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 169 bits (427), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 131/432 (30%), Positives = 224/432 (51%), Gaps = 28/432 (6%) Query: 8 TGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMQEWLRML 67 T ++H LS+ L +++ G LE +DF +Y F+A+ + K FFP+D E++ ++ Sbjct: 2 TTTTTNAKHSKLST----VLRVTS-GNFLEMFDFFLYGFYASYISKTFFPSD-NEFVSLI 55 Query: 68 QTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAA 127 TFG F AG+L RP+G II+ Y D +GR++ +++ +MA+ TL+I +P YA+IGVAA Sbjct: 56 LTFGTFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAA 115 Query: 128 PILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFI 187 PIL+L R LQG + G E+ G V++AE F V +A I+ +L+G + Sbjct: 116 PILVLTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLV 175 Query: 188 NSAFEPGEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANELPLKTVL 247 + + P +++ + WRIPF +G + +RR L+ET E AR++ + + + + Sbjct: 176 SQSLSPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQET---DEFLARKRHPSFGEIMSSI 232 Query: 248 REHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLS-PKLVMQANCAGTLMLTIGCL 306 ++ V M + + ++ + TP++ + V LS ++ C G L+ C Sbjct: 233 AQNWKIVVAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVG---LSNFCW 289 Query: 307 --AIGWLCDRIGTRASMIIGWGGLGLST-----WWFYGQLPGSPESLVWGYALVGLFVGS 359 +G L DR+G R +++ + L L T W + S ++L+ + S Sbjct: 290 LPIMGALSDRVGRRP-VLVAFTLLTLLTAYPVMSWLVSDI--SFKNLLITELWLSFLYAS 346 Query: 360 ISLTPIVS-TRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWI---KADPQGPALYVI 415 + +V+ T P VR G S AY++A A+FGG TP L+S W+ D P ++ Sbjct: 347 YNGAAVVALTEVMPAHVRTVGFSLAYSLATAIFGGFTP-LVSTWLIETSGDKAAPGYWMS 405 Query: 416 GLSILGVLLAFV 427 ++ G+L V Sbjct: 406 FAAVCGLLATLV 417 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,413,382 Number of extensions: 61252 Number of successful extensions: 365 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 15 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)