BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0043.p (543 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspi... 1117 0.0 gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspi... 428 e-121 gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 95 2e-20 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 91 2e-19 >gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 1117 bits (2888), Expect = 0.0, Method: Compositional matrix adjust. Identities = 537/543 (98%), Positives = 541/543 (99%) Query: 1 MNSNNKAEVVIVGTGVVGVLIAEQLLDAGIPVLMLEAGPRVARAEVVENFRNLPLALKGD 60 MNSNNKAEVVIVGTGVVGVLIAEQLL+AGIPV MLEAGPRVARAEVVENFRNLPLALKGD Sbjct: 1 MNSNNKAEVVIVGTGVVGVLIAEQLLNAGIPVRMLEAGPRVARAEVVENFRNLPLALKGD 60 Query: 61 PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED 120 PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED Sbjct: 61 PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED 120 Query: 121 MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP 180 MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP Sbjct: 121 MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP 180 Query: 181 GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL 240 GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL Sbjct: 181 GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL 240 Query: 241 EAKGATILTDAVVYRVDTDASNRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS 300 EAKGATILTDAVVYRVDTDA++RVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS Sbjct: 241 EAKGATILTDAVVYRVDTDANDRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS 300 Query: 301 ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH 360 ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH Sbjct: 301 ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH 360 Query: 361 AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL 420 AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL Sbjct: 361 AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL 420 Query: 421 SGKKDWLGLPRPAIHYDVGDYTRKSAELYSLPIGRKIAEAMGATDIKVSPKFAQSKHIMG 480 S KKDWLGLPRPAIHYDVGDYTR+SAELYSLPIGRKIAEAMGATDIKVSPKFAQSKHIMG Sbjct: 421 SSKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAMGATDIKVSPKFAQSKHIMG 480 Query: 481 GTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILRQL 540 GTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILRQL Sbjct: 481 GTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILRQL 540 Query: 541 QKA 543 QKA Sbjct: 541 QKA 543 >gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 428 bits (1100), Expect = e-121, Method: Compositional matrix adjust. Identities = 236/545 (43%), Positives = 320/545 (58%), Gaps = 13/545 (2%) Query: 1 MNSNNKAEVVIVGTGVVGVLIAEQLLDAGIPVLMLEAGPRVARAEVVENFRNLPLALKGD 60 M++ N A+VV++G GV G L+A +L G VL+LE+GPR+ R ++VE FR+ A K D Sbjct: 1 MSNLNSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSS--AFKSD 58 Query: 61 PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED 120 + YP P APHP + E + YL+ GP + +IR GG++WHWA WR P D Sbjct: 59 FMAPYPYSPLAPHPRYTPE--PNGYLQQKGPHAFNAQYIRMFGGTSWHWAAQLWRYVPND 116 Query: 121 MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP 180 R HSLYGVG+DW YD LE YY + E G+ G + R P+PM +P S Sbjct: 117 FRQHSLYGVGKDWPISYDDLEDYYYQAEVIAGVGGSPESG----SPRKHPFPMERVPASW 172 Query: 181 GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL 240 +QR T + A + + + RNS YD RPACC NNNC+P+CP+ A+Y A + Sbjct: 173 LQQRVTARL-APEFTVLDDSTGRNS-RNYDGRPACCGNNNCMPLCPIDAQYHGGLAAQQA 230 Query: 241 EAKGATILTDAVVYRVDTDASNRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS 300 E G ++T+AVVYR++ D R+ A +Y+D NK+SHRIT + FVLA N IETPKLLLMS Sbjct: 231 EDSGVKVITEAVVYRLEHDEKGRIAAAHYYDWNKVSHRITAQTFVLAANAIETPKLLLMS 290 Query: 301 ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH 360 + R P G+AN D VGRN+ DHP + ++ + E W G GPV I + G FR+EH Sbjct: 291 ISDRFPQGIANARDNVGRNLCDHPAIGVSFDVDEEIWPGRGPVSPCSIGQFRDGDFRAEH 350 Query: 361 AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL 420 A +N ++ VT + +G G+ L+E+I A I A E LPD NR+TL Sbjct: 351 APFRIDISNASQVAAVTKEVVAEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTL 410 Query: 421 SGKKDWLGLPRPAIHYDVGDYTRKSAELYSLPIGRKIAEAMGATDIKVSPK--FAQSKHI 478 KKD LGLP PA++++V DY K E + IA +GAT+IK S F+ +HI Sbjct: 411 GDKKDELGLPVPALYWNVDDYVIKGTE-QTRACYDAIAAKLGATNIKHSKSGAFSNRQHI 469 Query: 479 MGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILR 538 G MGLDPA SV D RAHDH+NL++ G G MP+ N T++ AL L+ AD I+ Sbjct: 470 TGTLSMGLDPATSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKIIE 529 Query: 539 QLQKA 543 + + A Sbjct: 530 ESRHA 534 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 94.7 bits (234), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 143/618 (23%), Positives = 228/618 (36%), Gaps = 118/618 (19%) Query: 3 SNNKAEVVIVGTGVVGVLIAEQLLDAGIPVLMLEAGP-----------------RVARAE 45 +N+ +VVIVG G G L+A +L AG+ V LE GP R + Sbjct: 5 TNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPEDFAYPKPADQYGYATRNK 64 Query: 46 VVENFRNLPLALKGDPSS-CYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGG 104 V R+ L ++ + + P R W + GG Sbjct: 65 VFATPRDAALTVRYNTAQQALPTRKWGAFAPGTG-----------------------VGG 101 Query: 105 STWHWAGTCWRLTPEDMRLHSLYGVG------------RDWAFGYDVLEPYYTRVEHQLG 152 S HW R TP D++L + +D+ F ++ +EP+Y + G Sbjct: 102 SGLHWTAVLIRATPTDLKLKTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCG 161 Query: 153 ICG----------PSDPALQWPPQRSAPYPMPALPFSPGEQRFTEVVQALGYRNIPTAQA 202 + G P + P RS PYP+PAL + F +V + LGY P A Sbjct: 162 LSGTTGNLRGQIQPGGDPFEGP--RSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSA 219 Query: 203 RNSGM---PYDDRPA------CCANNNCV---PVCPVGAKYDAATALARLEAKGATILTD 250 S PY + A C+ C+ P D L + K + Sbjct: 220 NVSQAWTNPYGAQIAPCNYCGFCSKYPCLNYSKASPQTTILDVVKRLPNFDYK-----VN 274 Query: 251 AVVYRVDTDASNRV-EAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMSANSRNPHGL 309 A V RVD A + V Y D N KI +L+ +L+L+S + P+ Sbjct: 275 ANVIRVDLHADKKTARGVTYVDENMKEVFQPAKIVILSSFQFANVRLMLLSGIGK-PYDP 333 Query: 310 ANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIM--ETSQGAF----RSEHAGA 363 + +GRN TL + +++ +G M + S G F + G Sbjct: 334 ITETGVIGRNYAFLSNGGATLFFKDKHFNSFATAGATGEMFNDISPGNFDDGVKLGFIGG 393 Query: 364 YFRYNNFARNRFVTFA----------ALKKGLVGQALDEEIRRMTACTADIVLAH--EVL 411 +++ A + A K+G++ D ++ T H ++ Sbjct: 394 AKIHSSQASGAPIGTALPPGTPSWGRGWKEGMI-DWYDHSMKISITTTCMSYRGHFLDLD 452 Query: 412 PDAEN-------RLTLSGKKDWLGLPRPAIHYDVGDYTRKSAELYSLPIGRKIAEAMGAT 464 P+ ++ R+T +++ L L R Y + S EL ++E+ A Sbjct: 453 PNYKDPWGQPLLRMTFDWRQNELKLQR----YLRDIVLKISKELNP----DHMSESFLAL 504 Query: 465 DIKVSPKFAQSKHIMGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTIS 524 D S H +GG IMG P +S ++ ++ D N+++PGG A P N T + Sbjct: 505 DSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSWDVHNVFVPGGNAFPQNFQANPTAT 564 Query: 525 MAALGLKAADAILRQLQK 542 + A+ L AA AI Q K Sbjct: 565 IGAITLMAAHAIKTQYLK 582 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 91.3 bits (225), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 142/607 (23%), Positives = 233/607 (38%), Gaps = 96/607 (15%) Query: 2 NSNNK-AEVVIVGTGVVGVLIAEQLLDAGIPVLMLEAG------PRVARAEVV---ENFR 51 N N K +VVIVG G G ++A+++ +AG V+ LE G P A + + E + Sbjct: 3 NKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEYQQ 62 Query: 52 NLPLALKGDPSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAG 111 L + SS R +P + + + P + GG+ HW+G Sbjct: 63 RFKLFQNLNKSSFTFRRKTGDSAIPYRQ------IAMFKPGEGV-------GGAGLHWSG 109 Query: 112 TCWRLTPEDMRLHSLYG------------VGRDWAFGYDVLEPYYTRVEHQLGICGPS-- 157 WR+ PE++R+ S Y +DW Y+ LEPY+ E +G G + Sbjct: 110 CHWRILPEELRMRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYR 169 Query: 158 -------DPALQWPPQRSAPYPMPALPFSPGEQRFTEVVQALGYR--NIPTAQARNSGMP 208 + + RS +P+PA F + Q G +P+A N+ P Sbjct: 170 VGGKVVDESGNPFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSA---NAAAP 226 Query: 209 YDDRPAC----------CANNNCVPVCPVGAKYDAATALARLEAKGATILTDAVVYRVDT 258 Y ++ C C+ C + AL ++ + + V R++ Sbjct: 227 YVNQYGCQMGPCTFCGFCSGYACYNYSKASPNVNIMPALR--QSNLFELRSSCNVLRIEL 284 Query: 259 DAS-NRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMSANSRNPHGLANRSDQVG 317 D+S + V Y D N + I + + +L L+S + P+ + + VG Sbjct: 285 DSSRKKATGVTYVDINGDTVFQPASIVIASTFAYNNARLFLLSGIGK-PYDPVSNTGAVG 343 Query: 318 RNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGA----FRSEH--------AGAYF 365 RN+ T+ EP G +N I G F +H G Sbjct: 344 RNIAFQMMSTIN-AFFEP-----GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSP 397 Query: 366 RYNNFARNRFVTFAALKKGL--VGQALDEEIR--RMTACTADIVLAHEVLPDAENRLTLS 421 + N + ++ ++ A+ G G+A + ++ + + D+ A+ V D L Sbjct: 398 IWCNPSGSKPISGIAVPSGTPRWGRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLD-P 456 Query: 422 GKKDWLGLPRPAIHYDVGDYTRKSAELYSLPIGRKIAEAMGATDIKVSPK-FA------- 473 KD G P +D D + + Y KIA + A +IKV+ K F Sbjct: 457 NYKDAFGQPLLRFTFDWKDNDIRMSR-YVTDQAVKIAHELKAREIKVTVKDFGAAPDLRS 515 Query: 474 -QSKHIMGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKA 532 Q+ H GG MG DP+ SVV+ ++ D N++ G G N T + AL + Sbjct: 516 YQTTHWAGGVAMGTDPSTSVVNRYLQSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWS 575 Query: 533 ADAILRQ 539 A AI Q Sbjct: 576 ARAIREQ 582 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,752,725 Number of extensions: 77722 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 4 Length of query: 543 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 453 Effective length of database: 1,193,720 Effective search space: 540755160 Effective search space used: 540755160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)