BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0042.p (499 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 1021 0.0 gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein... 354 1e-98 gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 286 2e-78 gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 281 7e-77 gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein ... 255 6e-69 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 1021 bits (2639), Expect = 0.0, Method: Compositional matrix adjust. Identities = 499/499 (100%), Positives = 499/499 (100%) Query: 1 MHKTLLTVLLAALMAGSALAQTTDSNTTPSADATASAAAPAPAPAISPEAALIERGHYLA 60 MHKTLLTVLLAALMAGSALAQTTDSNTTPSADATASAAAPAPAPAISPEAALIERGHYLA Sbjct: 1 MHKTLLTVLLAALMAGSALAQTTDSNTTPSADATASAAAPAPAPAISPEAALIERGHYLA 60 Query: 61 IASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQFAAALRRGVR 120 IASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQFAAALRRGVR Sbjct: 61 IASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQFAAALRRGVR 120 Query: 121 ADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMNLRWSMKAWN 180 ADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMNLRWSMKAWN Sbjct: 121 ADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMNLRWSMKAWN 180 Query: 181 ALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPDLQLAGAQVGPWY 240 ALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPDLQLAGAQVGPWY Sbjct: 181 ALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPDLQLAGAQVGPWY 240 Query: 241 APNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHSFSRLTEEDLRAIAV 300 APNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHSFSRLTEEDLRAIAV Sbjct: 241 APNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHSFSRLTEEDLRAIAV 300 Query: 301 YIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIRGARIFSASCASCHG 360 YIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIRGARIFSASCASCHG Sbjct: 301 YIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIRGARIFSASCASCHG 360 Query: 361 YNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRETEQGGHVFMPPFGDQPNALNK 420 YNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRETEQGGHVFMPPFGDQPNALNK Sbjct: 361 YNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRETEQGGHVFMPPFGDQPNALNK 420 Query: 421 LSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPRSPLLLMARIGMGVAVAVLVLLL 480 LSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPRSPLLLMARIGMGVAVAVLVLLL Sbjct: 421 LSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPRSPLLLMARIGMGVAVAVLVLLL 480 Query: 481 LVGLMVALRKRPDPEHDWK 499 LVGLMVALRKRPDPEHDWK Sbjct: 481 LVGLMVALRKRPDPEHDWK 499 >gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein [Herbaspirillum seropedicae SmR1] Length = 453 Score = 354 bits (908), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 203/432 (46%), Positives = 255/432 (59%), Gaps = 42/432 (9%) Query: 49 EAALIERGHYLAIASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTE 108 E LI+RG YLAIA DC ACH+ P +GG I+SP+G I +TNITPSKT GIG+YT Sbjct: 29 EGQLIKRGEYLAIAGDCAACHSQKGRAPFSGGHPISSPLGTIYSTNITPSKTAGIGSYTR 88 Query: 109 EQFAAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPF 168 +QFAAALR+GVRADG+ LYPAMPYTAYA L+D DV ALY YFM+GVA VD A TDLPF Sbjct: 89 DQFAAALRQGVRADGSQLYPAMPYTAYARLNDEDVTALYTYFMQGVAAVDQAAPATDLPF 148 Query: 169 PMNLRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPD 228 P N+R SMKAWN LFL ++P P P +SAQW RG YL +G AHCSTCHTPR +LM E+ + Sbjct: 149 PFNIRLSMKAWNWLFLDKKPFEPDPSQSAQWNRGAYLVQGLAHCSTCHTPRNFLMAEQLE 208 Query: 229 LQLAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHSFS 288 +L G +G WYAP+IT +W+++ L YL +G A AAG M EAI SFS Sbjct: 209 RRLQGGSLGTWYAPDITPAALQAKPAWTREMLADYLASGHAANGATAAGPMLEAIEMSFS 268 Query: 289 RLTEEDLRAIAVYI-----------KDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTL 337 RL EDL A+A Y+ ++P QG + + L Sbjct: 269 RLRREDLEAMASYLLPPTAATAAPAVNKPPAGLTPVQGDDTP---------------QAL 313 Query: 338 SAESPEEIRGARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVD 397 +A SP GAR++ +CASCH GQG P L + N +N++ IL GV Sbjct: 314 AALSP----GARVYMNNCASCHNTAGQGLNG--LPPLQSHPVLRQPNGDNVVMAILEGVL 367 Query: 398 RETEQGGHVFMPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGP 457 E QG F + L++ VAA++NYLL+ G +V + V +R GG Sbjct: 368 PEHGQGMIGFA----------HTLNDEQVAAVTNYLLQDIGGSKVSVTTQRVATLRAGGA 417 Query: 458 RSPLLLMARIGM 469 SPLL +AR GM Sbjct: 418 ASPLLWLARAGM 429 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 286 bits (733), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 184/473 (38%), Positives = 255/473 (53%), Gaps = 61/473 (12%) Query: 1 MHKTLLTVLLAALMAGSALAQTTDSN-TTPSADATASAAAPAPAPAISPEAALIERGHYL 59 M K AA+M+ SAL SN PSAD L++RG YL Sbjct: 1 MIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQ-----------------LVQRGEYL 43 Query: 60 AIASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQFAAALRRGV 119 A A DC+ACHTA GKP AGGLAIA+P+G + ++NITP K +GIGNY+EE F ALR G+ Sbjct: 44 AKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGI 103 Query: 120 RADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMNLRWSMKAW 179 R DGA+LYPAMPY +YA + ADV+ALYAYFM GV R D+ +P+++RW + W Sbjct: 104 RKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIW 163 Query: 180 NALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEK-----PDLQLAGA 234 +F + P+ ++ +RG+YL EG HC CHTPRG MQEK L+G Sbjct: 164 RKVFAPAVAV-DGPEDNSPLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGG 222 Query: 235 QVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHSFSRLTEED 294 + + A N+ G+G+WS+ D+VA+L+TGR + A G MA+ + +S +TEED Sbjct: 223 VIDGYLANNLRGDGRDGLGNWSEADIVAFLKTGR-NSHSAAFGGMADVVANSTQYMTEED 281 Query: 295 LRAIAVYIKD-------RPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIRG 347 L A+A Y+K PA+A D K AL R S +SP G Sbjct: 282 LSAMAKYLKSLKPVKDGTPALAYDD-----------KTHQAL-----RKGSDQSP----G 321 Query: 348 ARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRE-TEQGGHV 406 A F +CA+CH +G+G D +PSL + N +LI +L G + T + Sbjct: 322 AMAFLNNCAACHRSSGKG-YDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQ 380 Query: 407 F-MPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPR 458 F MP FG ++LS+ +VA + ++ +G A++V A DV +R+ P Sbjct: 381 FAMPAFG------SRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVRKQLPE 427 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 281 bits (720), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 169/412 (41%), Positives = 226/412 (54%), Gaps = 32/412 (7%) Query: 52 LIERGHYLAIASDCIACHTAPRGKP-MAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQ 110 L+ RG YLA A DC+ACH+A GKP +GGLAI S G I +TNITP K HGIGNY+E Q Sbjct: 31 LVARGQYLARAGDCMACHSAA-GKPAYSGGLAIDSGHGIIYSTNITPDKEHGIGNYSEAQ 89 Query: 111 FAAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPM 170 F+AA+R GVRADG LYPAMPY +YA +SD D+ ALY YFM+GV PV + + FP Sbjct: 90 FSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVASTPPASSMSFPF 149 Query: 171 NLRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPDLQ 230 N+RW MK WN F ++P Q SA+ RG YL EG HC +CHTPRG M EK Sbjct: 150 NIRWGMKLWNVFFANDKPFREQDGWSAEIKRGAYLVEGLGHCGSCHTPRGVAMNEKASDS 209 Query: 231 -----LAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITH 285 L+G + W P++ P WS D+V YL+TGR A AG M+ + H Sbjct: 210 SQAQFLSGGDLNGWAVPSLRGMP-----HWSAQDIVDYLQTGR-NKTASVAGEMSLVVEH 263 Query: 286 SFSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEI 345 S S L EDL+A+A Y+K +A+ GSG QG + L+ Sbjct: 264 STSHLKREDLQAMAAYLKTLSPVAS---SGSGRVIPQGVDATVSKLTAAKDLTL------ 314 Query: 346 RGARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDR-ETEQGG 404 G R++ +CA+CH G+G+ G +P L + N + L+ IL G TE+ Sbjct: 315 -GERLYLDNCAACHFVTGRGA-PGIFPVLDGATVVNAENPSALLHVILAGARTPSTEKAP 372 Query: 405 HVF-MPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQG 455 + MP F ++LS+ + AAL+ ++ + +G A V +V +R G Sbjct: 373 SILVMPGFA------HRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAG 418 >gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 255 bits (651), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 167/439 (38%), Positives = 232/439 (52%), Gaps = 33/439 (7%) Query: 26 NTTPSADATASAAAPAPAPAISPEAALIERGHYLAIASDCIACHTAPRGKPMAGGLAIAS 85 + TP+AD TA A A + S E I RG YLA A DC+ACHT+ G P AGGLA+AS Sbjct: 57 HATPAAD-TALAQRIAAQGSASAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALAS 115 Query: 86 PVGEIIATNITPSKTHGIGNYTEEQFAAALRRGVRADGANLYPAMPYTAYAALSDADVQA 145 P+G I +TNITP K HGIG+++ E FA +R GV G +YPAMPY +Y+ L+D D+QA Sbjct: 116 PIGAIYSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQA 175 Query: 146 LYAYFMKGVAPVDVATRPTDLPFPMNLRWSMKAWNALFL-KEQPLPPQPQRSAQWLRGRY 204 LYAYF K V P R D+P+P+++RW + W +F P P + RG Y Sbjct: 176 LYAYFSKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAAGSDQELARGAY 235 Query: 205 LAEGPAHCSTCHTPRGWLMQEKP------DLQLAGAQ-VGPWYAPNITSHPSAGIGSWSQ 257 L EG HC +CH+PR MQEK L L+G Q V W P++ + GI WSQ Sbjct: 236 LVEGLGHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQ 295 Query: 258 DDLVAYLRTGRLEGRAQAAGSMAEAITHSFSRLTEEDLRAIAVYIKDRPAIATGDAQGSG 317 DLV +LRTGR + A + G+M + I S +++ DL A+A Y+ P Q + Sbjct: 296 ADLVEFLRTGRNQYTA-SFGAMNDVIEDSMQYMSDADLNAMARYLLSLPP----RQQAAP 350 Query: 318 SRFDQGKAGNALAQFRGRTLSAESPEEIRGARIFSASCASCHGYNGQGSRDGYYPSLFRN 377 R+D + A A + GR + P GARI+ CA+CH NG G +P+L N Sbjct: 351 YRYD---SATAQAAYDGRP---DGP----GARIYLDRCAACHRSNGTGYGKA-FPALAGN 399 Query: 378 SATAGVNANNLIATILYGVDRETEQGGH--VFMPPFGDQPNALNKLSNADVAALSNYLLK 435 +A + I IL G + + + M P+ L + VA +++Y+ Sbjct: 400 PVLQTSDATSAIRIILQGGRQPSTASATAGLVMAPYA------QLLDDQQVAEVTSYIQT 453 Query: 436 YYGKPAATVKAEDVEVIRQ 454 +G T A +V +R+ Sbjct: 454 AWGNRGGTTTAAEVAKVRK 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,534,564 Number of extensions: 69141 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 7 Length of query: 499 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 410 Effective length of database: 1,198,455 Effective search space: 491366550 Effective search space used: 491366550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)