BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0041.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 934 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 197 1e-51 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 192 5e-50 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 169 4e-43 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 166 4e-42 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 934 bits (2414), Expect = 0.0, Method: Compositional matrix adjust. Identities = 464/465 (99%), Positives = 464/465 (99%) Query: 1 MQSTQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 MQSTQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL Sbjct: 1 MQSTQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 Query: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL Sbjct: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 Query: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY 180 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY Sbjct: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY 180 Query: 181 MPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCS 240 MPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCS Sbjct: 181 MPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCS 240 Query: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG Sbjct: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 Query: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF Sbjct: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 Query: 361 YPELFPARARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSAL 420 YPELFPARARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSAL Sbjct: 361 YPELFPARARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSAL 420 Query: 421 AALSARETYRIPLEKLGDPNAQPLPRNEYDRLRAEAEVAGRYAKS 465 AALSARETYRIPLEKL DPNAQPLPRNEYDRLRAEAEVAGRYAKS Sbjct: 421 AALSARETYRIPLEKLDDPNAQPLPRNEYDRLRAEAEVAGRYAKS 465 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 197 bits (502), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 125/383 (32%), Positives = 200/383 (52%), Gaps = 16/383 (4%) Query: 6 SNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGV 65 ++ H Q+++A S IG+ALE++D IY A +I Q FFP GD + +++ + AT+GV Sbjct: 10 ADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQ-FFPTGDDSVSLLLTFATFGV 68 Query: 66 GYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMR 125 + RP+GA VLG D GRK L+ + LM T + +P+Y +GL APA + + + Sbjct: 69 SFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGK 128 Query: 126 LAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQ 185 + QGF+ GE +++ ++EH+P RRG+FSS+ + +LAA L + + EQ Sbjct: 129 MIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQ 187 Query: 186 FNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNMLR 245 WGWR+PF+ L+ AG IRR +DE P FK + + K +P+ D F +L Sbjct: 188 LASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSE---KTNAPLRDTFAHQKMRLLI 244 Query: 246 VVCMALMNVIPVVATIFGAAYAVQ----PAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGK 301 +M + V +++ YAV+ PA+ A V I +G+ P VG Sbjct: 245 GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGS-------PLVGA 297 Query: 302 LSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFY 361 LSD+IGR P +I S L + + ++ L ++ + GV+ Y A P+ Sbjct: 298 LSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALL 357 Query: 362 PELFPARARVSAMAIAQNLGTAI 384 ++FP R + M++A N+ I Sbjct: 358 ADIFPVATRGTGMSLAYNIAVTI 380 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 192 bits (488), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 126/403 (31%), Positives = 211/403 (52%), Gaps = 20/403 (4%) Query: 7 NAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVG 66 + +QQ ++A + IG+ LE++DF +Y+ A +I +LFFP G+ T+++ ++AT+GVG Sbjct: 13 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFA-VIIAKLFFPTGNDLTSLLLAVATFGVG 71 Query: 67 YVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRL 126 + RP+G +LG D GRK L + LM T +GL PTY+ +GL+AP ++V+ RL Sbjct: 72 FFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARL 131 Query: 127 AQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQF 186 QGF+ GE+ A++ + E++P R Y+SS+ + +L AAV + + A Sbjct: 132 LQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAAL 191 Query: 187 NVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNMLRV 246 WGWR+PFLL ++ G+ IR ++ETP F +E+ K SP+ + R+ Sbjct: 192 ASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFL---EEKDKTDSPLKEVIRNFPRETSAS 248 Query: 247 VCMALMNVIPVVATIFGAAYAVQPAYGIGFAK---DVYLWIPVVGNIVAVLVIPYVGKLS 303 M ++ + +F P Y + K +VG + +++ P VG+L+ Sbjct: 249 FSMVILWTVCTYVLLF-----YMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLA 303 Query: 304 DRIGRRPPIIVGSLLAGLFA---FAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 DRIGRRP + + L + A FAY+ + + L F L++G + Y + Sbjct: 304 DRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQ---LVFGTLIAAYTGPILAA 360 Query: 361 YPELFPARARVSAMAIAQNLGTAITAMLPALFTA--VAPPGSS 401 + ELFPA+ + +++A NL I + F +A GS+ Sbjct: 361 FSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGST 403 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 169 bits (429), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 112/329 (34%), Positives = 176/329 (53%), Gaps = 14/329 (4%) Query: 3 STQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLAT 62 +T + + KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L Sbjct: 7 NTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLA 65 Query: 63 YGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLV 122 + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L+ Sbjct: 66 FAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILI 125 Query: 123 IMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMP 182 I+R+ QG A+ GE GA++ + EH+P GRRG F+++ G L+ V L + + Sbjct: 126 ILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVG 185 Query: 183 AEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPN 242 F WGWRIPFL+S L++ IR +++E+P F E ++P+ +AF Sbjct: 186 EAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQW--K 243 Query: 243 MLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIP----- 297 L++V +AL+ + A ++ Y Q Y + F NI+ + Sbjct: 244 NLKLVILALVGLTAGQAVVW---YTGQ-FYALFFLTQTLKVDGPTANILIAAALLLATPF 299 Query: 298 --YVGKLSDRIGRRPPIIVGSLLAGLFAF 324 + G LSD+IGR+P I+ G L+A + F Sbjct: 300 FLFFGSLSDKIGRKPIILGGCLIAAVTYF 328 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 166 bits (420), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 114/381 (29%), Positives = 195/381 (51%), Gaps = 16/381 (4%) Query: 5 QSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYG 64 Q + Q+ AA +G+ALE+YDF +Y +++I +LFFP + T+++ +LAT+G Sbjct: 2 QPQLNKQRQVIAAI---VGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFG 57 Query: 65 VGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIM 124 +G+ RP+G +LG D GRK + + LM + + PTY +G+ AP ++V+ Sbjct: 58 IGFFMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVA 117 Query: 125 RLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAE 184 R+ QGFA GE S +++ ++E +P +RG + S+ L G A + + + E Sbjct: 118 RMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPE 177 Query: 185 QFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVF-KAADKEELKRRSPVVDAFRDCSPNM 243 + WGWR+PF+L L+ G IRR + ET F ++A+K +P+ D Sbjct: 178 ALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPN---AAPI--KLLDVVKTN 232 Query: 244 LRVVCMALMNVIPVVATIFGAAYAVQPAYG---IGFAKDVYLWIPVVGNIVAVLVIPYVG 300 LR V +++ VI F P + +G D + ++ + VIP+ G Sbjct: 233 LRGVLVSMGQVINGTVA-FYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAG 291 Query: 301 KLSDRIGRRPPIIVGSLLAGLFAFA-YLYAISIRNVELAFVMSILMWGVVYQGYNAVFPS 359 LSDR+GRR ++ SL L + +++ + ++ VM +L+ V+ + + P+ Sbjct: 292 ALSDRLGRRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPM-PT 350 Query: 360 FYPELFPARARVSAMAIAQNL 380 E FP R R + +A+A NL Sbjct: 351 ALSEQFPTRVRSTGLAVAYNL 371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,458,337 Number of extensions: 62054 Number of successful extensions: 348 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 13 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)