BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0040.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313057|ref|YP_003777149.1| porin signal peptide protein [H... 761 0.0 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 185 6e-48 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 124 1e-29 gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 112 3e-26 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 111 1e-25 >gi|300313057|ref|YP_003777149.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 761 bits (1964), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/380 (99%), Positives = 378/380 (99%) Query: 1 MTKPSIRTPVAMGLLLAASGSALAQSASNVTLYGTVDQYLGYMRSSSGKSLWALNDGAIL 60 MTKPSIRTPVAMGLLLAASGSALAQSASNVTLYGT+DQYLGYMRSSSGKSLWALNDGAIL Sbjct: 1 MTKPSIRTPVAMGLLLAASGSALAQSASNVTLYGTLDQYLGYMRSSSGKSLWALNDGAIL 60 Query: 61 RSRIGLKGYEDLGGGYATTFTLENGFSADTGAQADGARLFDRQSWVGIKTPAAGEFRIGR 120 RSRIGLKGYEDLGGGYATTFTLENGFSADTGAQADG RLFDRQSWVGIKTPAAGEFRIGR Sbjct: 61 RSRIGLKGYEDLGGGYATTFTLENGFSADTGAQADGTRLFDRQSWVGIKTPAAGEFRIGR 120 Query: 121 QNSVPFFIGGAIDYTERTTYGSIINTFGVPSRFDNDLSWKSPRWAGLQIDLHYAMPETAG 180 QNSVPFFIGGAIDYTERTTYGSIINTFGVPSRFDNDLSWKSPRWAGLQIDLHYAMPETAG Sbjct: 121 QNSVPFFIGGAIDYTERTTYGSIINTFGVPSRFDNDLSWKSPRWAGLQIDLHYAMPETAG 180 Query: 181 EGLGRRGVYQLGIDYINGPYRAGYAGLMAKPNAGGLYDNAVQYHNVYLNYDYGPGKVYLA 240 EGLGRRGVYQLGIDYINGPYRAGYAGLMAKPNAGGLYDNAVQYHNVYLNYDYGPGKVYLA Sbjct: 181 EGLGRRGVYQLGIDYINGPYRAGYAGLMAKPNAGGLYDNAVQYHNVYLNYDYGPGKVYLA 240 Query: 241 YVRSNNTTANANGNNAATILNNVSNPNNFFAGNDANVQRFYNVYQISADYRLTPQLRIGA 300 YVRSNNTTAN NGNNAATILNNVSNPNNFFAGNDANVQRFYNVYQISADYRLTPQLRIGA Sbjct: 241 YVRSNNTTANTNGNNAATILNNVSNPNNFFAGNDANVQRFYNVYQISADYRLTPQLRIGA 300 Query: 301 LYGVMVDTSHKEGGAQGGNVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLA 360 LYGVMVDTSHKEGGAQGGNVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLA Sbjct: 301 LYGVMVDTSHKEGGAQGGNVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLA 360 Query: 361 GADINGKSLVGLQMGILHRF 380 GADINGKSLVGLQMGILHRF Sbjct: 361 GADINGKSLVGLQMGILHRF 380 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 185 bits (469), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 130/368 (35%), Positives = 189/368 (51%), Gaps = 36/368 (9%) Query: 16 LAASGSALAQSASNVTLYGTVDQYLGYMRSSSGKSLWALNDGAILRSRIGLKGYEDLGGG 75 L +G ALAQS NVTLYG +D Y+GY ++ S+ +L+ G SR+G KG EDLGGG Sbjct: 12 LLCAGGALAQS--NVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGFKGSEDLGGG 69 Query: 76 YATTFTLENGFSADTGAQADGARLFDRQSWVGIKTPAAGEFRIGRQNSVPFFIGGAIDYT 135 F +ENGF++D+GA D RLF+RQSWVG+ GE R+GRQNS F + +D Sbjct: 70 LRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNE-LGELRLGRQNSPGFLMIARLDAF 128 Query: 136 ERTTYGSIINTFGV-PSRFDNDLSWKSPRWAGLQIDLHYAMPETAGEGLGRRGVYQLGID 194 TY S +N R+DN + + SP G ++ +Y + E R G L Sbjct: 129 AGATYASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAP---RNG---LSTA 182 Query: 195 YINGPYRAGYAGLMAKPNAGGLYDNAVQYHNVYLN--YDYGPGKVYLAYVRSNNTTANAN 252 + G Y +G L +A ++ + + + YDYG GKV+L Y R NN A A+ Sbjct: 183 MLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSGKVFLGYYRGNNLGAAAS 242 Query: 253 GNNAATILNNVSNPNNFFAGNDANVQRFYNVYQISADYRLTPQLRIGALYGVMVDTSHKE 312 N + +Y+ Y +SA+YRLT Q +GA YG D++ Sbjct: 243 ANTPGS---------------------YYSAYSLSANYRLTGQATLGAGYGWAKDSTGAG 281 Query: 313 GGAQGGNVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLAGADINGKSLVGL 372 A+ ++ YDLS+RT LY ++ N A + +G+A N A ++G ++ G+ Sbjct: 282 RNARQFSLIGTYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKN---APVSGGTVNGV 338 Query: 373 QMGILHRF 380 Q+GI H F Sbjct: 339 QVGIRHLF 346 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 128/435 (29%), Positives = 180/435 (41%), Gaps = 93/435 (21%) Query: 7 RTPVAMGLLLAASGSALAQSASNVTLYGTVDQYLGYMR--SSSGK---SLWALNDGAILR 61 ++ +A+ +L A +G+A AQS+S +T+YG +D L Y ++ GK SL A+ G + Sbjct: 3 KSLLALAVLGAFAGAAHAQSSS-ITIYGAIDASLTYTNKIATDGKNTGSLTAVESGLVKG 61 Query: 62 SRIGLKGYEDLGGGYATTFTLENGFSADTGAQADGARLFDRQSWVGIKTPAAGEFRIGRQ 121 SR+G+KG EDLGGG FTLENGF+ DTG G ++ RQ+ VG+ + G +GRQ Sbjct: 62 SRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGL-SGNFGTVLVGRQ 120 Query: 122 NSVPFFIGGAIDYTERTTYGSIINTFGVP-------------SRFDNDLSWKSPRWAGLQ 168 N IGG FGVP R +N + + SP + GL Sbjct: 121 NDPLNDIGGIAS----------AGGFGVPGTVHSKGLDRTDGQRLNNSIRYNSPNFNGLS 170 Query: 169 IDLHYAMPETAGEGLGRRGVYQLGIDYINGPYRAGYAGLMAK----------------PN 212 Y E G +G R + LG Y+NGP G +K N Sbjct: 171 FSGIYGFGEQVGRAVGGRSI-GLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACN 229 Query: 213 AGGLYDNA----VQYHNVYLNYDYGPGKVYLAYVRSNNTTANANGNNAATILNNVSNPNN 268 G D+A ++ + Y +GP KVY A+ A + V NN Sbjct: 230 GAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAK--------LHTKVRKDNN 281 Query: 269 FFAGNDANVQRFYN------------------VYQISADYRLTPQLRIGALYGVMVDTSH 310 F A DA YN + I Y+L P L L + T Sbjct: 282 FGAMYDAKTGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSL---TLMSAVQHTRA 338 Query: 311 KEGGAQGG-----NVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLAGADIN 365 K GA G N+GA YDLSKRT+ Y +++ S PG + Sbjct: 339 KFVGAASGRLLQLNLGAKYDLSKRTSAYALVRNLRSSDMYNVGLSKDQEPGID------- 391 Query: 366 GKSLVGLQMGILHRF 380 +S + G++H F Sbjct: 392 -RSQTAISTGLIHTF 405 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 112 bits (281), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 79/206 (38%), Positives = 106/206 (51%), Gaps = 8/206 (3%) Query: 28 SNVTLYGTVDQYLGYMRSSSGKSLWALNDGAILRSRIGLKGYEDLGGGYATTFTLENGFS 87 S+VTLYGTVD L Y + G S +N GA+ S++GL G EDLGGG F LENGF+ Sbjct: 35 SSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLENGFN 94 Query: 88 ADTGAQADGARLFDRQSWVGIKTPAAGEFRIGRQNSVPFFIGGAIDYTERTT-----YGS 142 ADTGAQ+ LF+RQS+VG+ + G+ +GRQ + F GA+ T T + Sbjct: 95 ADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGAHPG 154 Query: 143 IINTFGVPSRFDNDLSWKSPRWAGLQIDLHYAMPETAGEGLGRRGVY-QLGIDYINGPYR 201 I+ R +N +++ P GLQ + Y M E AG V L DY + Sbjct: 155 DIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAFSWS 214 Query: 202 AGYAGL--MAKPNAGGLYDNAVQYHN 225 AGY L +A N G + N + N Sbjct: 215 AGYTRLKNLANGNTAGSFGNVGSFAN 240 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 111 bits (277), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 123/393 (31%), Positives = 180/393 (45%), Gaps = 67/393 (17%) Query: 20 GSALAQSASNVTLYGTVDQYLGYMRSSS--GKSLWALNDGAILRSRIGLKGYEDLGGGYA 77 G+A AQS NVT+YG VD + Y+ +++ G S +++GAI SRIG KG EDLGGG Sbjct: 17 GAAFAQS--NVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLK 74 Query: 78 TTFTLENGFSADTGAQADGARLFDRQSWVGIKTPAAGEFRIGRQNSVPFFIGGAIDYTER 137 F LENGFS+DTGA G LF RQSWVG++ G+ R+GRQN+ F + D+ + Sbjct: 75 AMFQLENGFSSDTGAMQQGGSLFGRQSWVGLQ-GGFGKVRVGRQNTPLFDL--LADHFDP 131 Query: 138 TTYGSIINTFGVPS-----------RFDNDLSWKSPRWAGLQIDLHYAMPETAGEGLGRR 186 T G+ + +P+ R+D D P + +A E AG + Sbjct: 132 LTVGNYASNSWLPAAASLVRSPNMLRYDGDF---GP----VAASASWAFGEKAGS-VRTG 183 Query: 187 GVYQLGIDYINGPYR--AGYAGLMAKPNAGGLYDNAVQYHNVYLNYDYGPGKVYLAYVRS 244 + + Y GP GY ++ N+ + N+ +YD+G KV+ ++ Sbjct: 184 SQFSSALRYTAGPLALGGGYQQTVSSTNSSLKHTT----WNLSASYDFGVAKVFGGWLNI 239 Query: 245 NNTTANANGNNAATILNNVSNPNNFFAGNDAN-----VQRFYNVYQISADYRLTPQLRI- 298 + + G + + + N + AN V+R N + + + Y+ T L + Sbjct: 240 KDDS----GLTGSVMAADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLT 295 Query: 299 GALYGVMVDTSHKEGGAQGGNVG----------AYYDLSKRTTLYGFANYMKNQANAGWR 348 GA Y D S GNVG A Y LSKRT LY +Y K + A + Sbjct: 296 GAFY---YDRSKNVSYVSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYE 352 Query: 349 FSGSAGPGSNLAGADINGK-SLVGLQMGILHRF 380 + SA NGK SL + +GI H F Sbjct: 353 LTNSA-----------NGKNSLTTVGVGIRHIF 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,850 Number of extensions: 57569 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 14 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)