BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0030.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 530 e-152 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 127 6e-31 gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 124 9e-30 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 106 2e-24 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 104 9e-24 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 530 bits (1365), Expect = e-152, Method: Compositional matrix adjust. Identities = 256/263 (97%), Positives = 258/263 (98%) Query: 1 MKDHHPDILGREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAAN 60 MKDHHPDIL REDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAAN Sbjct: 1 MKDHHPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAAN 60 Query: 61 GKAFCAGHDLRQMQATPQQSYYQTLFHRCSQLMQSLRELPVPVIAKVQGLATAAGCQLVA 120 GKAFCAGHDLRQMQATPQQ+YY+ LFHRCS LMQSLRELPVPVIAKVQGLATAAGCQLVA Sbjct: 61 GKAFCAGHDLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVA 120 Query: 121 TCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGL 180 TCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGL Sbjct: 121 TCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGL 180 Query: 181 INQAVPAQELDGATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM 240 INQAVP QELD ATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM Sbjct: 181 INQAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM 240 Query: 241 DPEAQEGITAFLDKRKPNWAAPT 263 DPEAQEGITAFLDKRKPNWAAPT Sbjct: 241 DPEAQEGITAFLDKRKPNWAAPT 263 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 127 bits (320), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 85/258 (32%), Positives = 123/258 (47%), Gaps = 5/258 (1%) Query: 6 PDILGREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFC 65 P IL E++ GI +TLNRP N+ + M L+ + +++D +VR ++L G+ FC Sbjct: 4 PSIL-LENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFC 62 Query: 66 AGHDLRQMQATPQQS---YYQTLFHRCSQLMQSLRELPVPVIAKVQGLATAAGCQLVATC 122 AG DL P +++ L++SLR LP PVI V G+A AG L C Sbjct: 63 AGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLAC 122 Query: 123 DLAIAAESARFAVSGINVGLFCSTPAVA-LTRNLPIKRAFEMLVTGKFIDAATAAAWGLI 181 D+ +AA SA F +GL T L R + RA M + G+ I A A WGLI Sbjct: 123 DIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLI 182 Query: 182 NQAVPAQELDGATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMD 241 + V +L A+A P +A KA +Y LP+ + M Sbjct: 183 WKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRS 242 Query: 242 PEAQEGITAFLDKRKPNW 259 + +EG+TAFL+KR P + Sbjct: 243 RDYREGVTAFLEKRAPQF 260 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 124 bits (310), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 131/258 (50%), Gaps = 7/258 (2%) Query: 5 HPDILGREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAF 64 H DI R G+ T+TLNRP+ NA ++++ L L + D+ ++LA+ GK+F Sbjct: 6 HLDITPR----GLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKSF 61 Query: 65 CAGHDLRQMQATPQQSYYQTL--FHRCSQLMQSLRELPVPVIAKVQGLATAAGCQLVATC 122 AG DL M+ S L + + LM++L P P +AKVQG G LV+ C Sbjct: 62 SAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSCC 121 Query: 123 DLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGLIN 182 D+ +A+++A FA+S + +GL + + + + + +A V+ + AA AAA GL++ Sbjct: 122 DIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLVH 181 Query: 183 QAVPAQELDGATQALAREICSKPAISIARGKAMV-YRQQMMALPDAYAHAAEVMACNIMD 241 + P ELD T L + + ++ K +V + P+ + A V+A Sbjct: 182 EVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS 241 Query: 242 PEAQEGITAFLDKRKPNW 259 E +EG+ AFLDK P W Sbjct: 242 AEGREGLRAFLDKDAPAW 259 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 77/252 (30%), Positives = 118/252 (46%), Gaps = 4/252 (1%) Query: 5 HPDILGREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAF 64 + D+L + + LTLNRP NAL + L + A L + D +VR V+++ N KAF Sbjct: 7 YEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAF 66 Query: 65 CAGHDLRQMQATPQQSYYQTLFHRCSQLMQSLRELPVPVIAKVQGLATAAGCQLVATCDL 124 AG DL +M + T +Q +++ P P++A V G A AGC+L+ D+ Sbjct: 67 AAGADLNEM---IHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADI 123 Query: 125 AIAAESARFAVSGINVGLFCSTPAV-ALTRNLPIKRAFEMLVTGKFIDAATAAAWGLINQ 183 AIAA A+ INVG L R + A +M+++G+FI A A GL+ + Sbjct: 124 AIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAE 183 Query: 184 AVPAQELDGATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPE 243 V T ALA I K +++ K + + + L + + + Sbjct: 184 VVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASAD 243 Query: 244 AQEGITAFLDKR 255 QEGI AF +KR Sbjct: 244 RQEGIAAFQEKR 255 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 104 bits (259), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 73/250 (29%), Positives = 119/250 (47%), Gaps = 9/250 (3%) Query: 12 EDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAGHDLR 71 E + + + L+RP+ NALS+ ++T L L + + C+I+ + KAF AG D+ Sbjct: 9 ETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGADIS 68 Query: 72 QMQATPQQSYYQTLFHRCSQLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIAAESA 131 M ++ F ++ ++LR + PVIA V G A GC+L CD IAA++A Sbjct: 69 AMAGYDYMDVFKGEF--ITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNA 126 Query: 132 RFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAAAWGLINQAVPA 187 +F I +G+ P T+ LP +A ++ +TG+ +DA A GL+++ V A Sbjct: 127 KFGQPEIKLGI---VPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAA 183 Query: 188 QELDGATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPEAQEG 247 +L A A I P K V R L + + + + +EG Sbjct: 184 DKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEG 243 Query: 248 ITAFLDKRKP 257 + AFL+KR+P Sbjct: 244 MKAFLEKRQP 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.131 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 771,115 Number of extensions: 28706 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 10 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)