BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0025.p (730 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 1449 0.0 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 142 8e-35 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 137 2e-33 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 122 1e-28 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 108 1e-24 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 1449 bits (3751), Expect = 0.0, Method: Compositional matrix adjust. Identities = 729/730 (99%), Positives = 730/730 (100%) Query: 1 MTTPSSNTSASATVTPEAIPSAAQTARWMFDPKIVKPALADSFRKLSPRAQWRNPVMFVV 60 MTTPSSNTSASATVTPEAIPSAAQTARWMFDPKIVKPALADSFRKLSPRAQWRNPVMFVV Sbjct: 1 MTTPSSNTSASATVTPEAIPSAAQTARWMFDPKIVKPALADSFRKLSPRAQWRNPVMFVV 60 Query: 61 YLGSILTTLLFIQAAVGQGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAALRG 120 YLGSILTTLLFIQAAVGQGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAALRG Sbjct: 61 YLGSILTTLLFIQAAVGQGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAALRG 120 Query: 121 VKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGDTVPADAEVIEGV 180 VKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGDTVPADAEVIEGV Sbjct: 121 VKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGDTVPADAEVIEGV 180 Query: 181 ATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGA 240 ATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGA Sbjct: 181 ATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGA 240 Query: 241 RRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALIALLVCLIP 300 RRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALIALLVCLIP Sbjct: 241 RRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALIALLVCLIP 300 Query: 301 TTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMP 360 TTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMP Sbjct: 301 TTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMP 360 Query: 361 APGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIPFSAQTRVS 420 APGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIPFSAQTRVS Sbjct: 361 APGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIPFSAQTRVS 420 Query: 421 GIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVIEWHAEAAMIN 480 GIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVIEWHAEAAMIN Sbjct: 421 GIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVIEWHAEAAMIN 480 Query: 481 QGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLA 540 QGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLA Sbjct: 481 QGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLA 540 Query: 541 EATPEQKLALIRQHQSEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLD 600 EATPEQKLALIRQHQ+EGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLD Sbjct: 541 EATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLD 600 Query: 601 SNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGALNVMHLAS 660 SNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGALNVMHLAS Sbjct: 601 SNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGALNVMHLAS 660 Query: 661 PDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIKLI 720 PDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIKLI Sbjct: 661 PDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIKLI 720 Query: 721 DLLLSAFGLV 730 DLLLSAFGLV Sbjct: 721 DLLLSAFGLV 730 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 142 bits (359), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 159/583 (27%), Positives = 265/583 (45%), Gaps = 60/583 (10%) Query: 108 EGRSKAQAAALRGVKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAG 167 EGR+K Q A ++ + R VR DG + +V R GDLV++ G Sbjct: 209 EGRAKRQTADAIAALNALRPENAR----VRRDGQDIEVPLAQV------RVGDLVVLRPG 258 Query: 168 DTVPADAEVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGE 227 + VP D V EG + +DE+ +TGES P +++G +T G+ +V+ + E Sbjct: 259 ERVPVDGVVREGRSHLDEAMLTGESRPQAKQAG---DPLTAGSINAEGVLVMETTAIGAE 315 Query: 228 AFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVS 287 L R++ MVE A+ K P + L+ ++ VF+ + L ++ + Sbjct: 316 TVLARIVRMVEQAQAAKAP----IQRLVDQVSAVFVPAVLLLSLLTLLGWGLVQGNWEQA 371 Query: 288 VTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGT 347 + +A++V P +G +AG + ++ A+E A V+ + DKTGT Sbjct: 372 LLNAVAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGT 431 Query: 348 ITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLRE-----RE 402 +TLG+ + P EL +A E P GR++ A R Sbjct: 432 LTLGHPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRS 491 Query: 403 MANLGAEFIPFSAQTRVSGIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARR 462 +A LG E + V G R++ G++ +++L A PA +++ E + + Sbjct: 492 VAGLGIEGV-------VEG-----RQLMLGSSRWMQQLQAHA----PA-LQARAEQLQAQ 534 Query: 463 GGTPLVVIEWHAEAAMINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDN 522 G T + W A + + +LG + D +K G + L RMG+++ M++GDN Sbjct: 535 GRT----LSWLAR----RNDSGVEVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDN 586 Query: 523 RLTAAAIAAEAGVDDFLAEATPEQKL-ALIRQHQSEGKLVAMTGDGTNDAPALAQADVAV 581 A +A+E G+ + AE P K A+ Q ++VAM GDG NDAPALA ADV + Sbjct: 587 LGAAQQVASELGIQEVRAEVLPADKAEAVTALRQGGAQVVAMVGDGVNDAPALAAADVGM 646 Query: 582 AMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPA 641 AM++GT A +A + + +P + + ++I ++ T I ++ ++A I Sbjct: 647 AMSTGTDVAMQAAGVTLMHGDPGLVADAIDISRR--------TYAKIRQNL--FWAFIYN 696 Query: 642 MFVGSYPQLGALNVMHLASPDSAIMS-AVIFNALVIVALIPLA 683 + LG LN M LA A S +V+ NAL++ P A Sbjct: 697 LVGIPLAALGLLNPM-LAGAAMAFSSVSVVSNALLLRRWRPHA 738 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 167/605 (27%), Positives = 267/605 (44%), Gaps = 95/605 (15%) Query: 109 GRSKAQAAALRGVKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGD 168 G+++ AALR V S A+ +R DG + + E+V +GDL+L+E G Sbjct: 87 GKTERALAALR-VLNSPTARVIR-------DGRTMRIAAAELV------RGDLILLEEGA 132 Query: 169 TVPADAEVIEGVA-TVDESAITGESAPVIR------------ESGGDFSA--VTGGTRVL 213 VPADA V+E +DES +TGE+ PV + SG SA V GT V+ Sbjct: 133 RVPADAGVLEASNLQLDESLLTGEACPVEKFAPSTKPEPHALPSGQTDSATLVRSGTLVV 192 Query: 214 SDWIVVRVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFS 273 + +V+ L ++ +++ + +P + + ++ L + L+++ L+ Sbjct: 193 RGSGIAKVTATGARTELGKISSLLHELVEEPSPMQRHIARIVTVLAVTGLMLSALLVVLE 252 Query: 274 IFSVNAAGSGSPVSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEA 333 +S G + + IAL + L+P + +L+ G RM +ANV+ A+E Sbjct: 253 GYS---HGRWTEALLMG-IALSMSLLPEELPIILTIFPAIGAWRMARANVLTRRLSAIET 308 Query: 334 AGDVDVLLLDKTGTITLGNRQAAAFMPAP----------GVTE---RELADVAQLASLAD 380 G + VL DKTGT+T + M A G+T R L + A LAS Sbjct: 309 LGSISVLCTDKTGTLTENRMRVVQGMIAGRHMYFDESDIGITSPEWRTLLETAALASSPR 368 Query: 381 ET-PEGRSIVVLA---KNRFNLREREMANLGAEFIPFSAQTRVSGI------DLGHRRIR 430 T P ++ LA RF + L E+ PF+ R + D Sbjct: 369 PTDPMEKAFHQLAGESAKRFA--PPALTQLLQEY-PFTPALRAMTMVWKGDDDAPLLVAT 425 Query: 431 KGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVI-----EWHAEAAMINQGAPI 485 KGA +AI L PA+V+ I G L VI W + Sbjct: 426 KGAPEAIASLCGLT----PAEVQQMQNDIMHLGSHGLRVIAVAACNWSGALPASQAQFGL 481 Query: 486 RILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVD--------- 536 R LG + L D ++ G+ + + I+ +MITGD+ TA IA +AG+ Sbjct: 482 RYLGLLALADPLRPGVPDAIQRCHQAEIRVIMITGDHPSTAQNIARQAGISADQVLLGDE 541 Query: 537 ----------------DFLAEATPEQKLALIRQHQSEGKLVAMTGDGTNDAPALAQADVA 580 + A TP QKL ++ + +G+++AMTGDG NDAPAL A V Sbjct: 542 VQAMAEHELARRLRTTNVCARITPLQKLRIVAALKDDGEIIAMTGDGVNDAPALKAAHVG 601 Query: 581 VAMNS-GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQML-MTRGSLTTFSIANDVAKYFAI 638 +AM + GT A+EAG+++ LD N ++E + +G+++ RG++ + A+ Sbjct: 602 IAMGARGTDVAREAGSLILLDDNFASIVEGILMGRRIFSRMRGAMIYVLAMHMPIAGMAL 661 Query: 639 IPAMF 643 +P +F Sbjct: 662 LPVVF 666 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 122 bits (305), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 130/471 (27%), Positives = 225/471 (47%), Gaps = 59/471 (12%) Query: 160 DLVLIEAGDTVPADAEVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVV 219 D V + G+ VP D V+ G +++++ ITGES PV +++G V GT + Sbjct: 290 DTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG---DPVFAGTINEQGAFEM 346 Query: 220 RVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNA 279 RV+ G++ L R++ V+ A+ Q+ P + + A + +V + +L + + A Sbjct: 347 RVTAAQGDSTLARIVKSVQQAQGQRAPTQRFVDNF--ARYYIPAVVVMAVLVALLPPLLA 404 Query: 280 AGSGSPVSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDV 339 + P AL+ LLV P + V+G++ + ++ G +E + Sbjct: 405 GAAFQPWLYKALV-LLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRA 463 Query: 340 LLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAK------ 393 L LDKTGT+T G P VT DV L + AD+ + V LA Sbjct: 464 LALDKTGTLTFGK---------PRVT-----DVVSLQAQADQQALLQLAVSLAARSDHPV 509 Query: 394 NRFNLREREMANLG-AEFIPFSA---QTRVSG--IDLGHRRIRKGAADAIRKLTRAADQR 447 +R + E+A + +EF + Q R++G + LG+ R+ + +L + + Sbjct: 510 SRAIAAQEEIATVEVSEFAALAGRGVQGRINGQLLHLGNHRL-------VHELGQCS--- 559 Query: 448 LPAQVESDIESIARRGGTPLVVIEWHAEAAMINQGAPIRILGTVELKDIVKGGIKERFGE 507 ++E+ +++ ++G T V + G P + V + D V+ ++ E Sbjct: 560 --PELEARLQAFEKQGKTTTV---------LCLDGQPQLL---VAVADTVRPSSRQAVAE 605 Query: 508 LRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQ-HQSEG-KLVAMTG 565 LR +G++ +M+TGDN TA AIAA+ G+ D + PE KL I + Q G + V M G Sbjct: 606 LRALGVEVIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKAIEELSQRHGVRGVGMVG 665 Query: 566 DGTNDAPALAQADVAVAMN-SGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 615 DG NDAPALA+AD+ AM +GT A E ++ +D + K+ + + + ++ Sbjct: 666 DGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRK 716 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 108 bits (271), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 126/504 (25%), Positives = 213/504 (42%), Gaps = 69/504 (13%) Query: 156 LRKGDLVLIEAGDTVPADAEVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSD 215 LR+GD++L GDT+ ADA ++EG D + ++GES P + G + GG LS Sbjct: 342 LREGDVILARPGDTIAADACLLEGAGEFDLALLSGESRPQSFQIGQE---APGGAVNLSQ 398 Query: 216 WIVVRVSVNPGEAFLDRMIAMVEGARRQKTP----NELALTLLLVALTLVFLLVTVTLLP 271 + +RV GE+ L + + E A + K ++ + +VAL LL T L Sbjct: 399 PVFLRVVRATGESTLAALTRLAEQAGQGKPALAQWADVVASRFVVAL---LLLAGATFLA 455 Query: 272 FSIFSVNAAGSGSPVSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAV 331 + + A S + IA+LV P + + A R+++A + G + Sbjct: 456 WQLIDPTRAWSTA-------IAVLVVSCPCALSLATPSALAAATDRLLRAGTLIVRGNVL 508 Query: 332 EAAGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVL 391 E D ++ DKTGT+T G QL +L + RS+ + Sbjct: 509 ETLQRADTIVFDKTGTLTQGR--------------------PQLTALWSDVDCQRSLAIA 548 Query: 392 AKNRFNLREREMANLGAEFIPFSAQTRVSGIDLGHRRIRKGAADAIRKLTRAADQRLPAQ 451 A + + L + A+ +V ++ + +LT + Sbjct: 549 AA----MERGSLHPLAKALVEEGARRQVDALE-------------VEQLTSVTGAGMQGM 591 Query: 452 VESDIESIARR------GGT--PLVVIEWHAEAA-MINQGAPIRILGTVELKDIVKGGIK 502 +E D+ I R GT P V+++ A A + G+ L +L D ++ Sbjct: 592 IEGDLYRIGSRSFVAGLAGTALPPVLLQSAASGAGSVYLGSSDGWLARFDLADALRTDAV 651 Query: 503 ERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQHQSEGKLVA 562 R +G++T++++GD A +AA AG+ + A TP+ KL +R Q +G +VA Sbjct: 652 ATVAAFRALGLRTILLSGDQPEVCAEVAAAAGISEVQAACTPQGKLDYVRNLQRDGAVVA 711 Query: 563 MTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ-MLMTRG 621 + GDG NDA L DV+ AM G A+ + + V + + + + ++ M + R Sbjct: 712 IVGDGINDAAMLRAGDVSFAMGKGAALAQVSADAVIMSDRLQAVADCAAMAQRTMHIVRQ 771 Query: 622 SLTTFSIANDVAKYFAIIPAMFVG 645 +L + N F IPA G Sbjct: 772 NLVWAATYN-----FLAIPAAAFG 790 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,038,846 Number of extensions: 81716 Number of successful extensions: 464 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 442 Number of HSP's successfully gapped: 8 Length of query: 730 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 638 Effective length of database: 1,184,250 Effective search space: 755551500 Effective search space used: 755551500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)