BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0021.p (716 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein... 1445 0.0 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 151 2e-37 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 150 5e-37 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 145 9e-36 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 142 1e-34 >gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 1445 bits (3741), Expect = 0.0, Method: Compositional matrix adjust. Identities = 714/716 (99%), Positives = 715/716 (99%) Query: 1 LNKKKAPSPPNLSSLITLGVVVTVLVTTVSVLILVDQFTRGYARSEVETRLKQLAWQMQD 60 LNKKKAPSPPNLSSLITLGVVVTVLVTTVSVLILVDQFTRGYARSEVETRLKQLAWQMQD Sbjct: 23 LNKKKAPSPPNLSSLITLGVVVTVLVTTVSVLILVDQFTRGYARSEVETRLKQLAWQMQD 82 Query: 61 ALERGMAEREIDIRLLVSRPSIRQAQDPAHIRAVLEELQRNVPHYAWIGMARPDGTVLAA 120 ALERGMAEREIDIRLLVSRPSIRQAQDPAHIRAVLEELQRNVPHYAWIGMARPDGTVLAA Sbjct: 83 ALERGMAEREIDIRLLVSRPSIRQAQDPAHIRAVLEELQRNVPHYAWIGMARPDGTVLAA 142 Query: 121 TKGLLEGMSVAERPWFQQGQQTEYAADYHPAVLLEKKLPPQSDPWRFVDLSMPVLNMQGQ 180 TKGLLEGMSVAERPWFQQGQQTEYAADYHPAVLLEKKLPPQSDPWRFVDLSMPVLNMQGQ Sbjct: 143 TKGLLEGMSVAERPWFQQGQQTEYAADYHPAVLLEKKLPPQSDPWRFVDLSMPVLNMQGQ 202 Query: 181 MIGVMGMHLSWGWAREIASKLLNPTSSRYDVDVLIVRSDGTVILGPKDMEETKIDTASFR 240 MIGVMGMHLSWGWAREIASKLLNPTSSRYDVDVLIVRSDGTVILGPKDMEETKIDTASFR Sbjct: 203 MIGVMGMHLSWGWAREIASKLLNPTSSRYDVDVLIVRSDGTVILGPKDMEETKIDTASFR 262 Query: 241 AAQHSSAGGSLIETWPDDRRYVTGYARTGQGGEMKLKWTVIVRQPETVALEAFRDLERRV 300 AAQHSSAGGSLIETWPD RRYVTGYARTGQGGEMKLKWTVIVRQPETVALEAFRDLERRV Sbjct: 263 AAQHSSAGGSLIETWPDGRRYVTGYARTGQGGEMKLKWTVIVRQPETVALEAFRDLERRV 322 Query: 301 IVVGALAGAVLVLLAFMLTRRLVAPINALSAALEQRAAGLQEVKIPLVTSVRELQLLSSA 360 IVVGALAGAVLVLLAFMLTRRLVAPINALSAALEQRAAGLQEV+IPLVTSVRELQLLSSA Sbjct: 323 IVVGALAGAVLVLLAFMLTRRLVAPINALSAALEQRAAGLQEVEIPLVTSVRELQLLSSA 382 Query: 361 LGTMVEREERYLREMRSLNESLEERVVERTSRLHETATALQQALDQQQTNQIQLEESAAE 420 LGTMVEREERYLREMRSLNESLEERVVERTSRLHETATALQQALDQQQTNQIQLEESAAE Sbjct: 383 LGTMVEREERYLREMRSLNESLEERVVERTSRLHETATALQQALDQQQTNQIQLEESAAE 442 Query: 421 LRAILENAQDAFIAADEHGHIVEWNRQAELLLGWPRDTVMGRSMAELIIPSDMRAAHNHG 480 LRAILENAQDAFIAADEHGHIVEWNRQAELLLGWPRDTVMGRSMAELIIPSDMRAAHNHG Sbjct: 443 LRAILENAQDAFIAADEHGHIVEWNRQAELLLGWPRDTVMGRSMAELIIPSDMRAAHNHG 502 Query: 481 MAQFLKDGTSVVINNRIELQALHRDGHELPVEITVGHVKRQSGHLFIAFLHDITERLAFR 540 MAQFLKDGTSVVINNRIELQALHRDGHELPVEITVGHVKRQSGHLFIAFLHDITERLAFR Sbjct: 503 MAQFLKDGTSVVINNRIELQALHRDGHELPVEITVGHVKRQSGHLFIAFLHDITERLAFR 562 Query: 541 DQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEA 600 DQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEA Sbjct: 563 DQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEA 622 Query: 601 GDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFEL 660 GDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFEL Sbjct: 623 GDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFEL 682 Query: 661 THSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKNRIELGRVGDF 716 THSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKNRIELGRVGDF Sbjct: 683 THSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKNRIELGRVGDF 738 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 151 bits (382), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 106/313 (33%), Positives = 169/313 (53%), Gaps = 24/313 (7%) Query: 409 TNQIQLEESAAELR--AILENAQDAFIAADEHGHIVEWNRQAELLLGWPRDTVMGRSMAE 466 T++ Q+E AELR A+ ++++ + D + I+ N+ + G+ + ++G+ Sbjct: 465 TDRKQIE---AELRISAVAFDSREGMLITDANSKILRVNKAFTEITGYSAEEIVGKH--- 518 Query: 467 LIIPSDMRAAHNHGMAQF--LKDGTSVVINNRIELQALHRDGHELPVEITVGHVKRQSGH 524 P+ +R+ HG A F +++ + E+ ++G P +T+ VK + Sbjct: 519 ---PNLLRS-DRHGPAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQ 574 Query: 525 L--FIAFLHDITERLAFRDQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALF 582 + +++ HDI++R +++RELA D LT LPNR DRL + + + R AL Sbjct: 575 VTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALL 634 Query: 583 FMDLDGFKGINDRYGHEAGDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQS-QQ 641 F+DLD FK +ND GHE GD LL + A+RL A+VR+ DTVAR+ GDEFVV+LE L QQ Sbjct: 635 FIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQ 694 Query: 642 DAIM----VAEKILHAIREPFELTHSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAM 697 +A + EKIL A+ ++L ++SIG V+ L +EL+ AD AM Sbjct: 695 EAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGATVF---LGRAASVDELMKQADLAM 751 Query: 698 YAAKRLGKNRIEL 710 Y AK G+N + Sbjct: 752 YKAKETGRNALRF 764 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 150 bits (378), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 120/399 (30%), Positives = 192/399 (48%), Gaps = 19/399 (4%) Query: 318 LTRRLVAPINALSAALEQR-AAGLQEVKIPLVTSVRELQLLSSALGTMVEREERYLREMR 376 L LV A +A +R A Q++ + E +L++ +G + +L + Sbjct: 80 LNMPLVTMYQAYESAFRERIAVRYQDLAASFFCASGEQELIARQIGDVFNDFNTHLGSII 139 Query: 377 SLNESLEERVVERTS---RLHETATALQQALDQQQTNQIQLEESAAELRAILENAQDAFI 433 E +E+ + ++S LH L L + Q ++ S + R +++N+ D + Sbjct: 140 GFAEMVEDELSRKSSGQQSLHLLMQNLTALLKAEHAIQQRIRSSERQFRTLVDNSPDVIL 199 Query: 434 AADEHGHIVEWN----RQAELLLGWPRDTVMGRSMAELIIPSDMRAAHNHGMAQFLKDGT 489 D G V N R+ L+ +T++ RS D + + Q G Sbjct: 200 RYDMEGRCVFANPAFTRETGLMADQDINTILDRSANRRW--DDESCSFGERVRQVAATGV 257 Query: 490 SVVINNRIELQALHRDGHELPVEITVGHVKRQSGHLF--IAFLHDITERLAFRDQMRELA 547 + V+ L+ H GH + E+ + +F +A D+T R ++R A Sbjct: 258 ADVV----VLEWRHPQGHLVSHEMHIVPEYDADEQIFGTLAIGRDVTRRKEVEFKLRHQA 313 Query: 548 LTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEAGDELLVE 607 D L+ LPNRR D L + A++ LA+ F+DLD FK +ND GH GD+LLV Sbjct: 314 NYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVGDQLLVV 373 Query: 608 FARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFELTHSTVHI 667 A+R++ASVR+ D VARLAGDEFV +L L++ + + I+ A+ EPF+L V I Sbjct: 374 AAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAALAEPFQLGEHRVRI 433 Query: 668 SSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKN 706 S+S+G+A+Y D D L+ AD+AM+AAK G+N Sbjct: 434 SASVGIAIYPHDAD---TVTTLIGCADQAMFAAKGGGRN 469 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 145 bits (367), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 79/169 (46%), Positives = 108/169 (63%), Gaps = 4/169 (2%) Query: 538 AFRDQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYG 597 A +++ ++ L D LT LPNR DRL + + +A R AL FMDLDGFK IND G Sbjct: 257 AANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLG 316 Query: 598 HEAGDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREP 657 H GD LLVE A+RL +S R DTVARL GDEF VIL +L + +DA+ VAEK++ A+ +P Sbjct: 317 HHVGDRLLVEVAQRLTSSSRANDTVARLGGDEF-VILVDLNTPEDAMGVAEKLVEAVNQP 375 Query: 658 FELTHSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKN 706 F++ + +S+S+G+A+Y D +L+ AD AMY KR G+N Sbjct: 376 FKVDQHELRVSASVGIAIYPED---GATRHDLVINADAAMYHTKRSGRN 421 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 142 bits (358), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 75/166 (45%), Positives = 104/166 (62%), Gaps = 3/166 (1%) Query: 541 DQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEA 600 D++ A DVLT LPNR +DR+ + ARR + A+ FMDLDGFK IND GH Sbjct: 256 DKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLDGFKAINDSLGHAV 315 Query: 601 GDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFEL 660 GD LLV A+R++ +R D VAR+ GDEFVV++ NL S ++E IL A+R+ F++ Sbjct: 316 GDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLSENILAALRQDFQI 375 Query: 661 THSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKRLGKN 706 +T+ ++SSIG+AVY D + L+ AD AMY AK+ G+N Sbjct: 376 DDATLRVTSSIGIAVYPNSGD---SVDALMKNADAAMYEAKQSGRN 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,999,291 Number of extensions: 80058 Number of successful extensions: 416 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 362 Number of HSP's successfully gapped: 40 Length of query: 716 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 624 Effective length of database: 1,184,250 Effective search space: 738972000 Effective search space used: 738972000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)