BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0020.p (526 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [H... 1052 0.0 gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [H... 172 5e-44 >gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 527 Score = 1052 bits (2720), Expect = 0.0, Method: Compositional matrix adjust. Identities = 523/526 (99%), Positives = 523/526 (99%) Query: 1 LYSKTCLGGMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWL 60 LYSKTCLGGMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWL Sbjct: 2 LYSKTCLGGMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWL 61 Query: 61 ISEPGRAPVAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQ 120 ISEPGRAPVAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQ Sbjct: 62 ISEPGRAPVAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQ 121 Query: 121 RFASGLPLPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQGK 180 RFASGLPLPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQGK Sbjct: 122 RFASGLPLPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQGK 181 Query: 181 VLKQSALAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLMV 240 VLKQSALAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLMV Sbjct: 182 VLKQSALAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLMV 241 Query: 241 QLSSGRVYNLPPPTQGVSALMIIGLFDRLGVTQADGFAHLHGLVEATKRAFILRNQHVTD 300 QLSSGRVYNLPPPTQGVSALMIIGLFDRLGVTQADGFAHLHGLVEATKRAFILRNQHVTD Sbjct: 242 QLSSGRVYNLPPPTQGVSALMIIGLFDRLGVTQADGFAHLHGLVEATKRAFILRNQHVTD 301 Query: 301 PAHMRVDAAQWLLAGALDQEARRIDMQRAAPWPHPARPGDTIWMGAADAQGRVVSYIQSI 360 PAHMRVDAAQWLLAGALDQE R IDMQRAAPWPHPARPGDTIWMGAADAQGRVVSYIQSI Sbjct: 302 PAHMRVDAAQWLLAGALDQEVRHIDMQRAAPWPHPARPGDTIWMGAADAQGRVVSYIQSI 361 Query: 361 FWEFGSGVVVPDTGIVWQNRGASFILDEGVNQLQPGKLPFHTLNPSLAQLDDGRTLAFGT 420 FWEFGSGVVVPDTGIVWQNRGASF LDEGVNQLQPGKLPFHTLNPSLAQLDDGRTLAFGT Sbjct: 362 FWEFGSGVVVPDTGIVWQNRGASFTLDEGVNQLQPGKLPFHTLNPSLAQLDDGRTLAFGT 421 Query: 421 MGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTNLKLEGRIPEATIQAL 480 MGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTNLKLEGRIPEATIQAL Sbjct: 422 MGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTNLKLEGRIPEATIQAL 481 Query: 481 RRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRADGVCAGL 526 RRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRADGVCAGL Sbjct: 482 RRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRADGVCAGL 527 >gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 172 bits (437), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 148/542 (27%), Positives = 234/542 (43%), Gaps = 35/542 (6%) Query: 9 GMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWLISEPGRAP 68 GMV+ LA + G IL++GGNAV+A VA +AVV P+ IGG GF +I + Sbjct: 55 GMVATEQELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGK 114 Query: 69 -VAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQRFASGLP 127 VA+ A A + Y + +P R + L V V G A L HA + Sbjct: 115 NVALDFREMAPQRATRNMYLDDKGNVVPGRSLYTHLAV--GVPGTVAGLSHALSKYGTMK 172 Query: 128 LPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQ-GKVLKQSA 186 L ++ A+ A+ G PV+RS ++ + L A + +G P Q G++L Q Sbjct: 173 LSQVIQPAVELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQKD 232 Query: 187 LAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLMVQLSSGR 246 LA SL +A+ G FY G +A+ + A + G + DL+ ++ P+ + Sbjct: 233 LAKSLRLIAKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRGYQ 292 Query: 247 VYNLPPPTQ-GVSALMIIGLFDRLGVTQ--ADGFAHLHGLVEATKRAFILRNQHVTDPAH 303 V ++PPP+ GV + ++ + +R + Q AD +H + E K A+ R++++ DP Sbjct: 293 VMSMPPPSSGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDPDF 352 Query: 304 MRVDAAQWLLAGALDQEARRIDMQRAAP-------WPHPARPGDTIWMGAADAQGRVVSY 356 +V D+ A++I+ RA P P P T AD G +V+ Sbjct: 353 TKVPVKGLTSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLVAT 412 Query: 357 IQSIFWEFGSGVVVPDTGIVWQNRGASFILDEGV-----------NQLQPGKLPFHTLNP 405 ++ FGSG+V TGI N F GV N + P K P +++P Sbjct: 413 TYTLNLNFGSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSMSP 472 Query: 406 SLAQLDDGRTLAFGTMGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTN 465 + D L G+ GG T + N+ A PR + W Sbjct: 473 TFVLKDGKPFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPR--IHHQWA--PDQ 528 Query: 466 LKLEGRIPEATIQALRRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRA-DGVCA 524 L++E + T++ L+ G ++ V MG + + DG +G +DPR DG Sbjct: 529 LRVEKGLSADTLKILQDKGQKISV----QPSMGRTQTIQIRSDGF-DGFSDPRNPDGRTL 583 Query: 525 GL 526 G Sbjct: 584 GF 585 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,693,172 Number of extensions: 72083 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 2 Length of query: 526 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 436 Effective length of database: 1,193,720 Effective search space: 520461920 Effective search space used: 520461920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)