BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0238.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 955 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 300 2e-82 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 208 9e-55 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 102 8e-23 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 101 1e-22 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 955 bits (2469), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/475 (99%), Positives = 474/475 (99%) Query: 1 MSPPTPAVGGWTLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFR 60 MSPPTPAVGGWTLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFR Sbjct: 1 MSPPTPAVGGWTLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFR 60 Query: 61 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGS 120 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGS Sbjct: 61 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGS 120 Query: 121 PVSSASLRTSQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAA 180 PVSSASLRTSQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAA Sbjct: 121 PVSSASLRTSQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAA 180 Query: 181 AAAVVMTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAM 240 AAAVV+TALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAM Sbjct: 181 AAAVVLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAM 240 Query: 241 LDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRL 300 LDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRL Sbjct: 241 LDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRL 300 Query: 301 SRIVENILFLAQADHAALAVERSALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQAN 360 SRIVENILFLAQADHAALAVER+ALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQAN Sbjct: 301 SRIVENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQAN 360 Query: 361 LIMWRRAVSNLVINAVRYGQAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFY 420 LIMWRR VSNLVINAVRYG+AESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFY Sbjct: 361 LIMWRRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFY 420 Query: 421 RGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAAVPS 475 RGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAAVPS Sbjct: 421 RGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAAVPS 475 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 300 bits (767), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 184/470 (39%), Positives = 280/470 (59%), Gaps = 18/470 (3%) Query: 12 TLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ 71 +L+ R+ ALF L+ +++ LG YL S L +R D+ L G+ +L+ ++E Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 Query: 72 -MEQRPALFESMMGNEQD-VRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRT 129 + +R + +++ G++Q VRI R G +P T P G +S +R Sbjct: 68 ILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDP--------TRTPPGIDLSQVDIRA 119 Query: 130 -----SQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAV 184 S + +R R+V++ V + G+ ++ ++ YR + AA Sbjct: 120 WDSIQSWKLGDLRARYVAS-KPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGA 178 Query: 185 VMTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRL 244 ++ L+ + L+R GL+PL M+ + ++ + L RLD + P EL + SFN MLDRL Sbjct: 179 LLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRL 238 Query: 245 EAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIV 304 + L+QFS DLAH++RTP+N LM Q+QVALG+ R +EY+ +L SN+EE +RLSR++ Sbjct: 239 HRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMM 298 Query: 305 ENILFLAQADHAALAVERSALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMW 364 E++LFLA+A++A + ++ LD+A+EL RI DYFEGIADE +R VQ AG+ QA+ + Sbjct: 299 ESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLL 358 Query: 365 RRAVSNLVINAVRYGQAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDR 424 RRAV NLV NA+RY A+ IR+ ++ G+G I V N + A+ + R+FDRFYR D Sbjct: 359 RRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADP 418 Query: 425 SRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIR-FSLRFPSAAAV 473 SRS+ S+GLGLAIV++IM LHGG A+V +PG + FSLRFP +AV Sbjct: 419 SRSSAGGSSGLGLAIVRSIMMLHGGQASV-TSSPGQLTVFSLRFPGRSAV 467 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 208 bits (529), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 113/274 (41%), Positives = 169/274 (61%), Gaps = 8/274 (2%) Query: 184 VVMTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDR 243 +V+ + + + R GL PL MS RA +P LSAR+ E MP EL +AL+FN LDR Sbjct: 178 IVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDR 237 Query: 244 LEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRI 303 +E Y L F+ D+AHE+RTP+ L+G++QVAL + RS E++ VL+SN+EEL R+ I Sbjct: 238 IEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSI 297 Query: 304 VENILFLAQADHAALAVERSALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIM 363 V ++LFLA++D A R +A+E+ + D+FE + D+ GMR +V AG +A +M Sbjct: 298 VNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEV--AGDTEATAMM 355 Query: 364 ----WRRAVSNLVINAVRYGQAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRF 419 ++RA++NL+ NA+++ + +RI E R+ V N + + + R+FDRF Sbjct: 356 ETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRF 415 Query: 420 YRGDRSRSAFTES--NGLGLAIVKAIMALHGGTA 451 YR D +R S +GLGLAIVKA+ +HGG A Sbjct: 416 YRVDSARQDINGSHGHGLGLAIVKAVARMHGGRA 449 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 102 bits (253), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 82/301 (27%), Positives = 147/301 (48%), Gaps = 17/301 (5%) Query: 176 IAGAAAAAVVMTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVAL 235 + GA A + L+ +RRGL+PL+++ + Q L A +D G P EL+ + + Sbjct: 166 LDGALTFASIFVVLVA---VRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIV 221 Query: 236 SFNAMLDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVE 295 + N ++ R+ G F D+AH++RTP+ L Q ++ + + + + L Sbjct: 222 AMNELMARINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNS 281 Query: 296 ELQRLSRIVENILFLAQADHAAL---AVERSALD-LAEELGRITDYFEGIADER-GMRFD 350 ++R+ R +L LA+++ +E +LD L EE + + E +R + FD Sbjct: 282 SVERMIRQSRQLLALARSEPGLFESRKLEELSLDKLVEE--SVQHFIEEADKKRIDLGFD 339 Query: 351 VQAAGQCQANLIMWRRAVSNLVINAVRYGQAESTIRILAESDGEGSRIMVENRSEDVTAQ 410 ++ A + + + + + NL+ NAVRY T+ + + + + + VE+ + + Sbjct: 340 LRPA-RLRGDRFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPE 398 Query: 411 AMARMFDRFYRGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGW-IRFSLRFPS 469 +FDRFYR + +GLGLAIV+ I HGG +V+C G F++RFP Sbjct: 399 HRELIFDRFYRANDK----VAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPL 454 Query: 470 A 470 A Sbjct: 455 A 455 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 115/432 (26%), Positives = 193/432 (44%), Gaps = 39/432 (9%) Query: 67 YNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPD---HLVPPAMT--------- 114 Y EQ +QR E M+ + + +R G FI+ NP L+ PA+ Sbjct: 45 YLNEQAQQR---MEDMVPRLE--QAYREHGSWDFIRDNPRAWFELMKPAIVEHNGAARSA 99 Query: 115 -PLPVGSPVSSASLRTSQRADGVRVRWVSALADVGKGG-------DTVEI----VAAYVM 162 P S ++ A+LR S D R R V +VG D V I + Sbjct: 100 GPTFTASDLTGATLRFSL-LDASR-RPVIGYPNVGPDARLRPLLQDDVIIGWVAQTPFES 157 Query: 163 VQEARMMRSYRWRIAGAAAAAVVMTALLGFLLLRRG---LQPLEAMSQRAAQITPDHLSA 219 V A +R + + A AVV L FL++R +PL +++ ++ + Sbjct: 158 VIGALDLRFQQSQFAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAI 217 Query: 220 RLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQ 279 R++ E E+ +A FN + LE +F D++HE+RTP+ +L GQ + Sbjct: 218 RVEVES-GDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDG 276 Query: 280 RRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALAVERSALDLAEELGRITDYFE 339 P+ Q L S E++ L+++V ++ L+ AD AL R+ +DLA L + Sbjct: 277 VLQPDA--QTLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYS 334 Query: 340 GIADERGMRFDVQ--AAGQCQANLIMWRRAVSNLVINAVRYGQAESTIRILAESDGEGSR 397 +RGMR + A+ + ++ +SNL N++RY A +++ A + Sbjct: 335 DRFSQRGMRLSCELPASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWH 394 Query: 398 IMVENRSEDVTAQAMARMFDRFYRGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPA 457 ++ ++ V + MAR+F+RF+R + SR+ + GLGLAI + I+ H GT Sbjct: 395 LVFDDSEPGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASP 454 Query: 458 PGWIRFSLRFPS 469 G +R + P+ Sbjct: 455 LGGLRVVVVLPA 466 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,388,722 Number of extensions: 55181 Number of successful extensions: 343 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 310 Number of HSP's successfully gapped: 30 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)