BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0237.p (223 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 449 e-127 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 291 2e-80 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 273 7e-75 gi|300313680|ref|YP_003777772.1| two component response regulato... 154 6e-39 gi|300313896|ref|YP_003777988.1| two component transcription res... 149 2e-37 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 449 bits (1154), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/223 (100%), Positives = 223/223 (100%) Query: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 Query: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM Sbjct: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 Query: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP 223 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP Sbjct: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP 223 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 291 bits (746), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 140/219 (63%), Positives = 176/219 (80%) Query: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 M IL++ED+PK+GDYLR+GL ESG+ VD + G DGLH A DY L++LDVMLPG++GW Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 ++ LR D PVLFLTARD V+DR++GL+LGADDYLVKPF++TEL+ R+RTLLRRG + Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 RE ++ E D+ LD +R KVTR G I LT KEF LLH+LI+R GE LSR IASQ+WDM Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 Query: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMF 219 NFDSDTNVVDVA++RLRAK+D F+KKL+HTV +GY+F Sbjct: 181 NFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 273 bits (698), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 134/223 (60%), Positives = 170/223 (76%), Gaps = 2/223 (0%) Query: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 M IL++ED+ K +YL KGL E G AVDLA NG DG H+A+E DYD++VLDVMLPGLDG+ Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 V+ ALR ++ PV+ LTARD V+DRI+GL GADDYL+KPFSF EL+ R++ L RRG Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 Query: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 +E I DLQ+D + K R G+ I LT KEF LL ++ +RQGE LS++ IA +WDM Sbjct: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 Query: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP 223 NFDS+TNVV+VAIKRLRAKIDAPF++KL+HTVR +GY+ EP Sbjct: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVL--EP 221 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 154 bits (389), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 85/219 (38%), Positives = 130/219 (59%), Gaps = 5/219 (2%) Query: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 M +L++EDDP G+ +RKGLR+ G+A+D ++G +DY +++LD+ LP DG Sbjct: 1 MRVLLVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGL 60 Query: 61 QVMSALRSKRD-LPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGV 119 V+ LR + + +PVL TARD V DR+ GL GADDYL+KPF EL R+R L RR Sbjct: 61 AVLRTLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQA 120 Query: 120 TREAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWD 179 R + ++ ++ L+ H+VT G + L+ +EF LL + R G LSR+ + +++ Sbjct: 121 GRAESLVQVREVILNPATHEVTVSGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLYG 180 Query: 180 MNFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYM 218 + ++N V+V I LR K+ + F I VR VGY+ Sbjct: 181 WDDSIESNAVEVHIHALRKKVGSDF----IKNVRGVGYL 215 >gi|300313896|ref|YP_003777988.1| two component transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 244 Score = 149 bits (376), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 88/226 (38%), Positives = 126/226 (55%), Gaps = 5/226 (2%) Query: 3 ILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGWQV 62 IL++ED+PK + L+K L +GY+ G L Q DL++LD+MLPG+DGW++ Sbjct: 18 ILIVEDEPKLAELLQKYLALAGYSARHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 Query: 63 MSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVK-PFSFTELVLRIRTLLRRGV-- 119 LR+ D+PVL LTAR +DR+RGL+LGADDY+ K PFS E+V R++++LRR Sbjct: 78 CRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRNALV 137 Query: 120 -TREAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIW 178 R A+ + L +D VRH+ + +G + LT E LL G+ SR + + + Sbjct: 138 QQRTAQAPARSLLHIDDVRHQASVRGEALNLTPLELRLLKTFASAPGQVFSREQLLNHLH 197 Query: 179 DMNFDSDTNVVDVAIKRLRAKIDAPFD-KKLIHTVRSVGYMFSEEP 223 D + VD IK LR K++ F I V VGY F P Sbjct: 198 DDDRSVTDRAVDTHIKNLRRKLEPCFPGHNAIQAVYGVGYCFELPP 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 659,112 Number of extensions: 26103 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 43 Length of query: 223 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 141 Effective length of database: 1,231,600 Effective search space: 173655600 Effective search space used: 173655600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)