BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0230.p (698 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312350|ref|YP_003776442.1| bacteriocin/lantibiotic exporte... 1392 0.0 gi|300312189|ref|YP_003776281.1| bacteriocin/lantibiotic (cyclol... 204 3e-53 gi|300311132|ref|YP_003775224.1| ABC-type bacteriocin/lantibioti... 194 1e-50 gi|300311488|ref|YP_003775580.1| multidrug ABC transporter ATPas... 130 4e-31 gi|300312861|ref|YP_003776953.1| multidrug ABC transporter ATPas... 129 5e-31 >gi|300312350|ref|YP_003776442.1| bacteriocin/lantibiotic exporter/double-glycine peptidase domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 730 Score = 1392 bits (3602), Expect = 0.0, Method: Compositional matrix adjust. Identities = 695/697 (99%), Positives = 696/697 (99%) Query: 1 MSEISTSLSWALTMAARRQGVVIDTQRLGTAIAAAVGKGGSPDIPKLCRALNLSRPRHLA 60 MSEISTSLSWALTMAARRQGVVIDTQRLGTAIAAAVGKGGSPDIPKLCRALNLSRPRHLA Sbjct: 33 MSEISTSLSWALTMAARRQGVVIDTQRLGTAIAAAVGKGGSPDIPKLCRALNLSRPRHLA 92 Query: 61 KPDRPSLPLLCQVKEQGWGLLVDQRPDGRWDFLCHTGQSVVGDAEIVGGGYRLDFTEQGG 120 KPDRPSLPLLCQVKEQGWGLLVDQRPDGRWDFLCHTGQSVVGDAEIVGGGYRLDFTEQGG Sbjct: 93 KPDRPSLPLLCQVKEQGWGLLVDQRPDGRWDFLCHTGQSVVGDAEIVGGGYRLDFTEQGG 152 Query: 121 KKGQQGFERVFLDAIGEFRPVLIEIVVASVVMSIIALGASIFSMQVYDRVIPTHGVATLI 180 KKGQQGFERVFLDAIGEFRPVLIEIVVASVVMSIIALGASIFSMQVYDRVIPTHGVATLI Sbjct: 153 KKGQQGFERVFLDAIGEFRPVLIEIVVASVVMSIIALGASIFSMQVYDRVIPTHGVATLI 212 Query: 181 VLAIGVGLAILFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQLPGSVGSLAG 240 VLAIGVGLAILFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQLPGSVGSLAG Sbjct: 213 VLAIGVGLAILFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQLPGSVGSLAG 272 Query: 241 QLRGYEQIRSFYTSQTLFTLVDLPLGLLFVLVIALIASPWMALVPTVFGVVAILIGYLSN 300 QLRGYEQIRSFYTSQTLFTLVDLPLGLLFVLVIALIASPWMALVPTVFGVVAILIGYLSN Sbjct: 273 QLRGYEQIRSFYTSQTLFTLVDLPLGLLFVLVIALIASPWMALVPTVFGVVAILIGYLSN 332 Query: 301 RKVARLADKSAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWIDVSAESIRSDMAMRA 360 RKVARLADKSAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWIDVSAESIRSDMAMRA Sbjct: 333 RKVARLADKSAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWIDVSAESIRSDMAMRA 392 Query: 361 IGDHAGYIAATLQQLSYSTTVIIGAFLVINGQITMGALIACSILGGRILAPVAMTPGLMV 420 IGDHAGYIAATLQQLSYSTTVIIGAFLVINGQITMGALIACSILGGRILAPVAM PGLMV Sbjct: 393 IGDHAGYIAATLQQLSYSTTVIIGAFLVINGQITMGALIACSILGGRILAPVAMIPGLMV 452 Query: 421 QRAHTKATLAGLEKIYALQTDQGSERQLTPDNLRGEFRIEEAKFTYAGSMLNAMTIDQLT 480 QRAHTKATLAGLEKIYALQTDQGSERQLTPDNLRGEFRIEEAKFTYAGSMLNAMTIDQLT Sbjct: 453 QRAHTKATLAGLEKIYALQTDQGSERQLTPDNLRGEFRIEEAKFTYAGSMLNAMTIDQLT 512 Query: 481 INAGERVAVLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTLNQHIGYLQQD 540 INAGERVAVLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTLNQHIGYLQQD Sbjct: 513 INAGERVAVLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTLNQHIGYLQQD 572 Query: 541 HRLFVGTLRENLLIGLPDPGDDVLRQVMERTGLLRLVSSHPKGLDLPIQEGGRGLSGGQK 600 HRLFVG+LRENLLIGLPDPGDDVLRQVMERTGLLRLVSSHPKGLDLPIQEGGRGLSGGQK Sbjct: 573 HRLFVGSLRENLLIGLPDPGDDVLRQVMERTGLLRLVSSHPKGLDLPIQEGGRGLSGGQK 632 Query: 601 QLVAFTRLLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVVTHKPAFLPMV 660 QLVAFTRLLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVVTHKPAFLPMV Sbjct: 633 QLVAFTRLLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVVTHKPAFLPMV 692 Query: 661 DRVIVVVGNRIVLDGPRDVILARLRGQQAPLPAVSAE 697 DRVIVVVGNRIVLDGPRDVILARLRGQQAPLPAVSAE Sbjct: 693 DRVIVVVGNRIVLDGPRDVILARLRGQQAPLPAVSAE 729 >gi|300312189|ref|YP_003776281.1| bacteriocin/lantibiotic (cyclolysin-type) secretion ABC transporter protein [Herbaspirillum seropedicae SmR1] Length = 726 Score = 204 bits (518), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 151/556 (27%), Positives = 277/556 (49%), Gaps = 12/556 (2%) Query: 131 FLDAIGEFRPVLIEIVVASVVMSIIALGASIFSMQVYDRVIPTHGVATLIVLAIGVGLAI 190 F+ A+ ++R L E+++ S+V+ +I L +F V D+V+ H + TL V+A+G+ A Sbjct: 152 FIPAVVKYRKTLAEVLLISLVLQLIGLITPMFFQVVMDKVLVNHAMMTLNVIAVGLIAAT 211 Query: 191 LFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQLP-GSVGSLAGQLRGYEQIR 249 +FE + R+ I S +D +L L+ LL++ + G ++R E IR Sbjct: 212 VFEAVLTGIRTAIFANASSKIDVELGSRLFSHLLSLPLSYFQVRRAGDSIARVRELENIR 271 Query: 250 SFYTSQTLFTLVDLPLGLLFVLVIALIASPWMALVPTVFGVVAILIGYLSNRKVARLADK 309 SF T + ++D+ +F+ V+ + +V V +L + RL DK Sbjct: 272 SFLTGNAMTLVLDIMFSFVFLGVMLWYSVSLSLIVLASIPVYVLLSLVFTPLLRERLNDK 331 Query: 310 -SAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWIDVSAESIRSDMAMRAIGDHAGYI 368 + +A N LVET+ G++T+K+ + ++ +W A + + + I A Sbjct: 332 FNKSAEN--QSFLVETLTGMDTVKAMAVEPRWQQKWDKQLAAYVAAGLTTTNISLLASGA 389 Query: 369 AATLQQLSYSTTVIIGAFLVINGQITMGALIACSILGGRILAPVAMTPGLMVQRAHTKAT 428 + +L + +GA LV+ ++++G L+A ++L G++ +P+ L + Sbjct: 390 VGLVSKLVTVGIMWLGALLVVKNEMSVGELVAFNMLAGQVASPILRLAQLWNDFQQIGIS 449 Query: 429 LAGLEKIYALQTDQGSERQLTPDNLRGEFRIEEAKFTYAGSMLNAMTIDQLTINAGERVA 488 ++ L + +T+ R P + G + F Y + L I GE + Sbjct: 450 MSRLADVLDARTEVAGNRTRLP-RVNGAISFDLVSFRYRPETPEVIRGLSLQIRPGEVIG 508 Query: 489 VLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTLNQHIGYLQQDHRLFVGTL 548 ++G G+GKST+ +L+ LY + GRI +DG D+A I+ +L Q IG + Q++ LF ++ Sbjct: 509 IVGRSGSGKSTLAKLVQRLYICETGRISVDGQDIAIIDTASLRQQIGVVLQENTLFSRSI 568 Query: 549 RENLLIGLPDPGDDVLRQVMERT---GLLRLVSSHPKGLDLPIQEGGRGLSGGQKQLVAF 605 R+N I L DP + Q++E G + P+G D + E G GLSGGQ+Q +A Sbjct: 569 RDN--IALADPSTPI-EQIIEAAKLAGAHDFICELPEGYDTMVGEQGSGLSGGQRQRIAI 625 Query: 606 TRLLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVVTHKPAFLPMVDRVIV 665 R L+ P + + DE T+++D + E+ + + Q I GRT++++ H+ + + DR+IV Sbjct: 626 ARALITNPGILIFDEATSALDYESEKIIQDNMRQ-ICAGRTVLIIAHRLSAVRDADRIIV 684 Query: 666 VVGNRIVLDGPRDVIL 681 + R+ G D ++ Sbjct: 685 MERGRVAESGSHDELI 700 >gi|300311132|ref|YP_003775224.1| ABC-type bacteriocin/lantibiotic ATPase/permease fusion exporter protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 194 bits (494), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 169/554 (30%), Positives = 284/554 (51%), Gaps = 14/554 (2%) Query: 131 FLDAIGEFRPVLIEIVVASVVMSIIALGASIFSMQVYDRVIPTHGVATLIVLAIGVGLAI 190 +L + + +EI+VA +V++++ L ++S +YD+VI H TL L +G+ L + Sbjct: 24 YLSLLRAYGSRFVEIIVAGIVVNVLGLLMPLYSRLIYDKVIGNHIPETLWALTLGMLLFV 83 Query: 191 LFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQLPGSVGSLAGQLRGYEQIRS 250 EL ++ R + +++ LD++ + R+L RV Q P VG + + R R Sbjct: 84 ALELVLRIIRVYYTEQLAGRLDSEFDEVSARRMLTARV-QAP--VGVVLARYRDLSAARD 140 Query: 251 FYTSQTLFTLVDLPLGLLFVLVIALIASP--WMALVPTVFGVVAILIGYLSNRKVARLAD 308 +S + LVDLP LL+++V+ LI W+ LV V L+ + A A Sbjct: 141 LMSSNYMLLLVDLPFLLLYLVVLGLIGGHMVWVLLVGGGLLVGLQLLLKVPGNDYANSAM 200 Query: 309 KSAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWI-DVSAESIRSDMAMRAIGDHAGY 367 K+ K L V G+ET+K+ + L ++ D SA ++ + R + + Sbjct: 201 KAGTG---KVDKLASIVYGMETLKTSPLQHRLLRAFLADASANAV-AQAKSRFWMNTSYA 256 Query: 368 IAATLQQLSYSTTVIIGAFLVINGQITMGALIACSILGGRILAPVAMTPGLMVQRAHTKA 427 I++ L T+++G +LV + +T+GALIACS+L R ++ + + + Sbjct: 257 ISSVGYTLISVATLVVGVYLVEDNLLTVGALIACSLLISRAATVLSSLAAFLGRIEMFRR 316 Query: 428 TLAGLEKIYALQTDQGSERQLTPDNLRGEFRIEEAKFTYAGSMLNAMTIDQLTINAGERV 487 A ++++ + +QG + ++RG ++ + LTI GE+V Sbjct: 317 ARAEFDQLFE-EPEQGPTADVLRQDMRGLIQVGNLLLHLDKKETPTLRNISLTIQPGEKV 375 Query: 488 AVLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTLNQHIGYLQQDHRLFVGT 547 ++G G+GKST+LR L+GL++ ++G +L+DG+ + + IG+ Q+ LF GT Sbjct: 376 GIVGRSGSGKSTLLRTLAGLHQAEEGHVLIDGVAVTAYAEEVRMRCIGFKPQEPFLFDGT 435 Query: 548 LRENLLIGLPDPGDDVLRQVMERTGLLRLVSSHPKGLDLPIQEGGRGLSGGQKQLVAFTR 607 + N+ +G PG V + + L L++ LD I+ G LSGGQ+Q+VA R Sbjct: 436 VAANIFVGDRVPG-QVYEAALAVSSLDDLIARGELRLDQLIKAPG-NLSGGQRQMVALAR 493 Query: 608 LLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVVTHKPAFLPMVDRVIVVV 667 ++ C P + LLDEPT +D+ E + L +GGRTLVV TH PA L +DR++V+ Sbjct: 494 VIACTPRILLLDEPTTGIDQQTEARIIERL-VGYAGGRTLVVATHSPALLRHMDRIVVID 552 Query: 668 GNRIVLDGPRDVIL 681 G RIV DGPR IL Sbjct: 553 GGRIVADGPRAQIL 566 >gi|300311488|ref|YP_003775580.1| multidrug ABC transporter ATPase/permease [Herbaspirillum seropedicae SmR1] Length = 758 Score = 130 bits (326), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 131/532 (24%), Positives = 253/532 (47%), Gaps = 42/532 (7%) Query: 171 IPTHGVATLIVLAIGVGLAILFELGMKFARSHIMDKVVSGLDAKLSQALYERLLAVRVDQ 230 I TH +ATL + + +G A+L G+ +AR++I+ V + A L Y LL + + Sbjct: 220 IDTH-LATLYLSGL-LGAAVL-AWGLGWARTYILALVSERIGADLRTTTYSHLLRLSQEY 276 Query: 231 LPGS-VGSLAGQLRGYEQIR-SFYTSQTLFTLVDLPLGLLFVLVIALI---ASPWMALV- 284 G G L ++ G E R + S L+D + +L +++ A I +PW+ALV Sbjct: 277 FGGKRTGDLMARI-GSETDRICVFLS---LHLLDFAIDVLMIIMTAAILISINPWLALVT 332 Query: 285 --PTVFGVVAILIGYLSNR------KVARLADKSAAASNLKTGLLVETVEGVETIKSGSG 336 P F +A +I + +R K+ R+ + T +L +T+ G+ +K+ + Sbjct: 333 LLPLPF--IAWMIHVVRDRLRHGFEKIDRIWSEV-------TNVLADTIPGIRVVKAFAQ 383 Query: 337 GWKFLSRWIDVSAESIRSDMAMRAIGDHAGYIAATLQQLSYSTTVIIGAFLVINGQITMG 396 + ++R+ + + ++ + + I L ++ + G + V + QIT+G Sbjct: 384 EKREVARFREANRHNLMVNDRVNKIWSLFTPTVTFLTEVGLLVVWVFGIWQVSSNQITVG 443 Query: 397 ALIACSILGGRILAPVAMTPGLMVQRAHTKATLAGLEKIYALQTDQGS----ERQLTPDN 452 L A R + ++ + T+ AG ++I+ + S + + Sbjct: 444 VLTAFLAYISRFYTRLDSMSRIV---SVTQKAAAGAKRIFDILDHVSSVPEPANPVHLEK 500 Query: 453 LRGEFRIEEAKFTYAG-SMLNAMTIDQLTINAGERVAVLGPIGAGKSTILRLLSGLYRPQ 511 ++G + F Y +++ M+ L+I GE + ++G G+GKST++ L+ Y Sbjct: 501 VQGAIEVRNIGFRYGNRAVIRNMS---LSIAPGEMIGLVGHSGSGKSTMVNLICRFYDVS 557 Query: 512 QGRILLDGLDMANINRLTLNQHIGYLQQDHRLFVGTLRENLLIGLPDPGDDVLRQVMERT 571 +G I +DG+D+ ++ ++IG + Q+ LF GT+ EN+ G P+ + + Sbjct: 558 EGAIRIDGVDIRSLPISEYRRNIGLVLQEPFLFFGTIAENIAYGKPEATREEIIAAARAA 617 Query: 572 GLLRLVSSHPKGLDLPIQEGGRGLSGGQKQLVAFTRLLLCQPNVFLLDEPTASMDEDLER 631 + P+G D + E G+ LSGG++Q ++ R LL P + +LDE T+S+D E+ Sbjct: 618 HAHEFILRLPQGYDSLVGERGQALSGGERQRISIARALLIDPRILILDEATSSVDTTTEK 677 Query: 632 HCLNVLNQEISGGRTLVVVTHKPAFLPMVDRVIVVVGNRIVLDGPRDVILAR 683 L+ + GRT + + H+ + L DR++V+ ++V G + ++AR Sbjct: 678 EIQKALDNLVQ-GRTTIAIAHRLSTLRKADRLVVLDRGQVVEVGNHEELMAR 728 >gi|300312861|ref|YP_003776953.1| multidrug ABC transporter ATPase/permease [Herbaspirillum seropedicae SmR1] Length = 578 Score = 129 bits (325), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 112/391 (28%), Positives = 187/391 (47%), Gaps = 18/391 (4%) Query: 299 SNRKVARLADKSAAASNLKTGLLVETVEGVETIKSGSGGWKFLSRWIDVSAESIRSDMAM 358 + +++ +L S A + T ++ ET + IK GG + + AE++RS M Sbjct: 178 TGKRLKKLNRDSLAVNAEMTQVIEETTRAQQVIKI-FGGQDYEKERFNRRAENLRS-YTM 235 Query: 359 RAIGDHAGYIAATLQQLSYSTTVIIGAFLVI--NGQITMGALIACSILGGRILAPVAMTP 416 R + + T + + V+I L+ NGQIT+G ++ +L P+ Sbjct: 236 RMTAAFSSTVPITQLMTATAVAVVIVMALIQSGNGQITVGGFVSFITAMLMLLTPLKQLA 295 Query: 417 GLM--VQRAHTKATLAGLEKIYALQTDQGSERQLTPDNL----RGEFRIEEAKFTYAGSM 470 + +QR +A E++Y L D+ +ER +T + L G+ E F Y G Sbjct: 296 EINGPLQRG-----MAAAEEVYKL-IDKTTER-ITGEALAGRATGQLDFVEVGFAYPGHE 348 Query: 471 LNAMTIDQLTINAGERVAVLGPIGAGKSTILRLLSGLYRPQQGRILLDGLDMANINRLTL 530 A+ L + AGE +A +G G GKST++ L+ G Y G I LDG D+ I+ +L Sbjct: 349 QLALKNINLHVEAGETIAFVGMSGGGKSTLVSLIPGFYSCSSGEIRLDGRDIETISLPSL 408 Query: 531 NQHIGYLQQDHRLFVGTLRENLLIGLPDPGDDVLRQVMERTGLLRLVSSHPKGLDLPIQE 590 Q I + Q+ LF TL N+ G +P + +E L +V+ P+GL + + Sbjct: 409 RQQIAMVNQNTVLFDDTLAANIAYGDANPDPARVEAAVEAAHLTDVVAGLPEGLQTRLGD 468 Query: 591 GGRGLSGGQKQLVAFTRLLLCQPNVFLLDEPTASMDEDLERHCLNVLNQEISGGRTLVVV 650 G LSGGQ+Q VA R + + +LDE T+++D + ER L++ + GRT V+ Sbjct: 469 NGSRLSGGQRQRVAIARAIYKDAPILILDEATSALDTESERAVQAALDR-LMQGRTTFVI 527 Query: 651 THKPAFLPMVDRVIVVVGNRIVLDGPRDVIL 681 H+ + + DR++V+ +I G +L Sbjct: 528 AHRLSTIERADRIVVLAHGQIAEIGTHQALL 558 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,126,609 Number of extensions: 93097 Number of successful extensions: 691 Number of sequences better than 1.0e-08: 113 Number of HSP's gapped: 589 Number of HSP's successfully gapped: 118 Length of query: 698 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 606 Effective length of database: 1,184,250 Effective search space: 717655500 Effective search space used: 717655500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)