BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0229.p
         (318 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm...   450   e-128
gi|300312344|ref|YP_003776436.1| hypothetical protein Hsero_3040...   100   1e-22
gi|300312348|ref|YP_003776440.1| centriolin protein [Herbaspiril...    98   1e-21
gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ...    71   9e-14
gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra...    57   2e-09

>gi|300312349|ref|YP_003776441.1| hemolysin-type secretion
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score =  450 bits (1157), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 223/225 (99%), Positives = 224/225 (99%)

Query: 1   MEMNNEKNPLISAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDG 60
           MEMNNEKNPLISAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDG
Sbjct: 1   MEMNNEKNPLISAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDG 60

Query: 61  GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNF 120
           GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNF
Sbjct: 61  GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNF 120

Query: 121 EPDLLDYKEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180
           EPDLLDYKEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV
Sbjct: 121 EPDLLDYKEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180

Query: 181 LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLAE 225
           LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQL +
Sbjct: 181 LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLED 225


>gi|300312344|ref|YP_003776436.1| hypothetical protein Hsero_3040
           [Herbaspirillum seropedicae SmR1]
          Length = 281

 Score =  100 bits (250), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 48/50 (96%), Positives = 50/50 (100%)

Query: 269 QLAFYRVVAVRNHLLSLGVPASRIRQRIEPVQAASQSGVEGLEIRIQKSE 318
           ++AFYRVVAVRNHLLSLGVPASRIRQRIEPVQAASQSGVEGLEIRIQKSE
Sbjct: 232 RMAFYRVVAVRNHLLSLGVPASRIRQRIEPVQAASQSGVEGLEIRIQKSE 281


>gi|300312348|ref|YP_003776440.1| centriolin protein [Herbaspirillum
           seropedicae SmR1]
          Length = 517

 Score = 97.8 bits (242), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 48/49 (97%), Positives = 49/49 (100%)

Query: 223 LAESQLQEIRTIRGEMCRQTDAILLATSRMRQASEPLAKGLETLQKQLA 271
           LAESQLQEIRTIRGEMCRQTDAILLATSRMRQASEPLAKGLETLQKQL+
Sbjct: 278 LAESQLQEIRTIRGEMCRQTDAILLATSRMRQASEPLAKGLETLQKQLS 326


>gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD
           family secretion protein [Herbaspirillum seropedicae
           SmR1]
          Length = 457

 Score = 71.2 bits (173), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 44/143 (30%), Positives = 75/143 (52%), Gaps = 3/143 (2%)

Query: 30  WAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEAVKQGQLLMVLEQDRVQ 89
           W+  + ID V R  G+++A    Q +Q  +GG V  ILV+EG AVK+GQ L+ +   R  
Sbjct: 58  WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117

Query: 90  TSLNDSRAKVAALRISLARLRAEVYG-HPLNFEPDLLDYKEYIANQKDLYVKRKILIDQD 148
             L + R K+  LR  +ARL AE  G   L    DL      + +++  +  R   + Q+
Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQE 177

Query: 149 LASLQDMLGLANQELEMNKSLER 171
           ++ L++    A ++ E+ +++ R
Sbjct: 178 MSVLREQ--SAQRQAELGEAISR 198


>gi|300312188|ref|YP_003776280.1| hemolysin secretion-related
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 447

 Score = 57.0 bits (136), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 29/73 (39%), Positives = 47/73 (64%)

Query: 12  SAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEG 71
           S +W  RL +  V+  LLW+ + +ID V  A G+VI  +RT+++ + D   V+ ILV EG
Sbjct: 58  SLRWTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEG 117

Query: 72  EAVKQGQLLMVLE 84
           + VK G++L+ L+
Sbjct: 118 QLVKAGEVLIELD 130


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.131    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 728,422
Number of extensions: 24278
Number of successful extensions: 152
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 5
Length of query: 318
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 233
Effective length of database: 1,217,395
Effective search space: 283653035
Effective search space used: 283653035
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 130 (54.7 bits)