BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0225.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 796 0.0 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 64 2e-11 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 60 3e-10 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 60 5e-10 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 59 9e-10 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 796 bits (2055), Expect = 0.0, Method: Compositional matrix adjust. Identities = 405/407 (99%), Positives = 405/407 (99%) Query: 2 GFTRYQKTVTGLLAFLQFAVILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASG 61 GFT YQK VTGLLAFLQFAVILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASG Sbjct: 15 GFTPYQKIVTGLLAFLQFAVILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASG 74 Query: 62 LLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAI 121 LLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAI Sbjct: 75 LLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAI 134 Query: 122 ATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVL 181 ATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVL Sbjct: 135 ATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVL 194 Query: 182 VRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNL 241 VRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNL Sbjct: 195 VRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNL 254 Query: 242 GIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLGKLRVFLFGAVLSIIMVLIYTHLGRVTV 301 GIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLGKLRVFLFGAVLSIIMVLIYTHLGRVTV Sbjct: 255 GIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLGKLRVFLFGAVLSIIMVLIYTHLGRVTV 314 Query: 302 PMIVAVNAVLFLGIFSRMIPFQALVSSVPAATQRGSFNAISASIQQLSGGVASVIAGHIV 361 PMIVAVNAVLFLGIFSRMIPFQALVSSVPAATQRGSFNAISASIQQLSGGVASVIAGHIV Sbjct: 315 PMIVAVNAVLFLGIFSRMIPFQALVSSVPAATQRGSFNAISASIQQLSGGVASVIAGHIV 374 Query: 362 TLAPDGRLGHFDIVGFVIVGTTLLASVLVWRLHRDLQARESAAATPA 408 TLAPDGRLGHFDIVGFVIVGTTLLASVLVWRLHRDLQARESAAATPA Sbjct: 375 TLAPDGRLGHFDIVGFVIVGTTLLASVLVWRLHRDLQARESAAATPA 421 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 39/137 (28%), Positives = 68/137 (49%) Query: 38 SMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSF 97 + +T G++VS YA A + A ++ RK+ L+ F+LG V C LA ++ Sbjct: 32 DLGVTAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQALVGLMCLFILGNVLCALAPNY 91 Query: 98 ESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLS 157 L++AR+VT I +A +L AP R + + ++ T + +LG+P+G Sbjct: 92 VFLMVARVVTAFCHAAFFGIGSVVAAELVAPQKRAQAVALMFTGLTVANILGVPLGTAFG 151 Query: 158 NRWNWHVPFLAMAALGL 174 + W F A+ A+G+ Sbjct: 152 HAAGWRSTFGAVTAIGV 168 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 60.1 bits (144), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 61/240 (25%), Positives = 106/240 (44%), Gaps = 31/240 (12%) Query: 12 GLLAFLQFAVILDFMLMSPLGALIMPSMQITPQQFGLVVS-----------AYAFSAGAS 60 G LA FA+ + +++PL +P M FG+ V A S+ S Sbjct: 27 GFLALGTFAIGTEGFMIAPL----LPRMA---ADFGMSVPMVASLVVIFTLVLALSSPIS 79 Query: 61 GLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLA 120 +L+A +R+R+L+ F + + SF LL+AR++ + G+ A Sbjct: 80 TVLSAAM----NRRRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASA 135 Query: 121 IATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALG---LAGG 177 +A + AP RGR + ++ + LG+P+G + + + W FLA++A+G LAG Sbjct: 136 LAGAMVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGI 195 Query: 178 LVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYL 237 L+ + R H+ +E A + +P+ A T L G + P+ + YL Sbjct: 196 LLGVPRQAGAHLHVAGLRERMA------VIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYL 249 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 60/249 (24%), Positives = 101/249 (40%), Gaps = 13/249 (5%) Query: 38 SMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSF 97 ++ ++ GL+V+ YA L R RK LL+ FV+G + C +A + Sbjct: 32 NLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLPRKTLLVALMVVFVVGNLACAIAPDY 91 Query: 98 ESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLS 157 +L++ R+V G + +AT L + + ++ T + VLG+P G +L Sbjct: 92 TTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQASAIALMFTGLTVANVLGVPFGTWLG 151 Query: 158 NRWNWHVPFLAMAALG---LAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLT 214 + W F A+ +G LA V++ R Q E + + L P+ + Sbjct: 152 QSYGWRATFWAVTVIGVIALAATAVLVPRSQ--------GGEETDFGKEVRVLMRPQVML 203 Query: 215 AFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLG 274 T L G F + + L G + + I L+ GL + G+ ADK Sbjct: 204 GLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFGLGLVIGNLAGGKLADK-- 261 Query: 275 KLRVFLFGA 283 KL L G+ Sbjct: 262 KLMATLLGS 270 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 58.5 bits (140), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 70/268 (26%), Positives = 117/268 (43%), Gaps = 33/268 (12%) Query: 61 GLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESL--------LLARIVTGLF-G 111 G+L +ADR RK L +GT GLA ++ES+ ++AR++ G G Sbjct: 79 GILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAG 138 Query: 112 GVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLSNR--------WNWH 163 G +GS T A T+ R IQ S + +LG +G +++ W W Sbjct: 139 GEMGSAT-AFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWR 197 Query: 164 VPFLAMAALGLAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAE-PRYLTA-FATTAL 221 +PFL +G G +R + L+++ L + PR +A F+ L Sbjct: 198 LPFLLGIIIGPVG---YYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVIL 254 Query: 222 LMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLGKLRVFLF 281 ++L+ + Y V L + +V GLC + P++GR AD++G+ R L Sbjct: 255 WTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGR-RPLLS 313 Query: 282 GAVLSIIMVLIYTHLGRVTVPMIVAVNA 309 G+ ++I++L Y PM +N+ Sbjct: 314 GSA-ALILMLAY--------PMFAYINS 332 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,181,222 Number of extensions: 46469 Number of successful extensions: 313 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 12 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)