BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0223.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 834 0.0 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 550 e-158 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 516 e-148 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 361 e-101 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 100 2e-22 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 834 bits (2154), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/404 (100%), Positives = 404/404 (100%) Query: 1 MSTSTPLRRLAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMA 60 MSTSTPLRRLAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMA Sbjct: 1 MSTSTPLRRLAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMA 60 Query: 61 VADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMA 120 VADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMA Sbjct: 61 VADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMA 120 Query: 121 EKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAF 180 EKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAF Sbjct: 121 EKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAF 180 Query: 181 GHSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSI 240 GHSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSI Sbjct: 181 GHSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSI 240 Query: 241 KAAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKM 300 KAAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKM Sbjct: 241 KAAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKM 300 Query: 301 KKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFYNKGSIRADGRNIHD 360 KKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFYNKGSIRADGRNIHD Sbjct: 301 KKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFYNKGSIRADGRNIHD 360 Query: 361 MYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLKQ 404 MYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLKQ Sbjct: 361 MYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLKQ 404 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 550 bits (1417), Expect = e-158, Method: Compositional matrix adjust. Identities = 258/378 (68%), Positives = 313/378 (82%), Gaps = 1/378 (0%) Query: 27 QVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVLGKPIEVISADHQNKADI 86 Q+S D ++IGFITD+SG Y+D DG GG EAI+MA+AD GG + GK IE++ ADHQNKAD+ Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 87 AATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINIGAGTARLTNEECTPYTV 146 AA++AREW D++G+DMLIGG NS TAL+ KV AEKK+VY+ IGAG+ARLTNEEC+PYTV Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 147 HYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLANDTSAVVKASGGTVVGSIKHP 206 HY YDTVALAKGT SAV+KQGGKSWFFL ADYAFGHSL +DT+ VVKASGGTVVG++KHP Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 207 PQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVTKTMKLVGLLMVINDIHA 266 +SD SSFLLQAQ+SKAQI+GLANAG D +NS+KAA EFG+TKT KL GLL+ IND+H+ Sbjct: 205 LGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 264 Query: 267 LGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIG 326 L L N EGL +TDSWYWD +E +R FA+R+F KKMPS+ QAA YSA YLK V+ IG Sbjct: 265 LTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIG 324 Query: 327 TDDSTKVMAQLKKTRIDDFYNK-GSIRADGRNIHDMYLYQVKSPAESKKPWDYLKLLETI 385 TDDS KVMA L+K + +D Y K G +RADGR +HDMYLYQVK+ +ESK PWDY KL++TI Sbjct: 325 TDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTI 384 Query: 386 PGEQAFTSVADSKCSLLK 403 P EQA+T+ A+SKC+ K Sbjct: 385 PAEQAWTTKAESKCAAWK 402 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 516 bits (1330), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/403 (61%), Positives = 310/403 (76%), Gaps = 4/403 (0%) Query: 5 TPLRRLAAACAIAGIFSGAAL---AQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAV 61 TP R AA A A GAA+ AQ + D ++IGFITDMSG YAD DG GG+EAIRMA+ Sbjct: 2 TPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAI 61 Query: 62 ADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAE 121 AD GG + GK +E++ ADHQNKADIAA +AREW D G+DML+GGVNSA +L+M KV E Sbjct: 62 ADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGE 121 Query: 122 KKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFG 181 KK+V+I++GAGT R TNEEC YT+ Y YDT ALA+GT +A+ +QGGKSW+FL ADYAFG Sbjct: 122 KKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFG 181 Query: 182 HSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIK 241 SL DTS VVK++GG VVG+ + P +SD SSFLLQAQ+SKAQILGLA AG D +N+IK Sbjct: 182 TSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIK 241 Query: 242 AAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMK 301 AA EFGV KTMKL GLL+ IND H+LGL+ +G+ +TD WYWD ++ +R +A R+F KMK Sbjct: 242 AANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKMK 301 Query: 302 KMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFY-NKGSIRADGRNIHD 360 KMPS QA SA YLKAV+A+G+ D+ KVM L+K RI+DF+ + G +R DGR +HD Sbjct: 302 KMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHD 361 Query: 361 MYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 MYL +VK P+ESK+PWDY KL++TIPGEQA+ + A+SKC+L K Sbjct: 362 MYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 361 bits (927), Expect = e-101, Method: Compositional matrix adjust. Identities = 195/400 (48%), Positives = 261/400 (65%), Gaps = 9/400 (2%) Query: 11 AAACAIAGIFSGAALAQVSNDA--VRIGFITDMSGPYADTDGLGGLEAIRMAVAD-FGGT 67 A AC ++ + A+ + DA V+IG +TDMSGPY+ G G + A +MAV D Sbjct: 14 AVACTVS--LAATAIPAWAQDANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNE 71 Query: 68 VLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYI 127 G IE++SAD+QNK D+ TRAREW D ++ + NS+ AL++ K++ +K + + Sbjct: 72 CKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAM 131 Query: 128 NIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLAND 187 G T LT EC P H+ +DT + A G A+A+ K G KSW+F+ DYAFGHSL D Sbjct: 132 YSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKD 191 Query: 188 TSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFG 247 S VVKA+GGTVVGS++HP +SD SSFLLQAQASKAQ++GLA+ G D VN+IK A+ F Sbjct: 192 ASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFA 251 Query: 248 V-TKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKKM-PS 305 V K +LV LL+ ++D+HALGL A+GL+ TD +YWD DE +R F++RF + K + P+ Sbjct: 252 VGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPT 311 Query: 306 THQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRI-DDFYNKGSIRADGRNIHDMYLY 364 QA YS+ +LKA A T D V ++++ I D SIR DGR IHDMYLY Sbjct: 312 MVQAGVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHDMYLY 371 Query: 365 QVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLKQ 404 QVK+PAESK PWDYLKLL IP +QAF + D+ C L+KQ Sbjct: 372 QVKTPAESKGPWDYLKLLSVIPAQQAFRPM-DAACPLVKQ 410 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 88/376 (23%), Positives = 164/376 (43%), Gaps = 12/376 (3%) Query: 31 DAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVLGKPIEVISADHQNKADIAATR 90 +++ G MSGP+A L +++A+ +G VLGK ++ D + K A + Sbjct: 29 ESIAFGCPVPMSGPFAANGKFADL-GMKLALQKYG-KVLGKSTSYVNLDTEGKPATAVRK 86 Query: 91 AREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINIGAGTARLTNEECTPYTVHYEY 150 +E + E+G GG+ S AL+M K + V+I AG +T +C T + Sbjct: 87 MQE-SIEKGTRFFAGGILSGEALAMGKEADKAGAVFITT-AGADEITGSDCNRATFRWSV 144 Query: 151 DTVALAKGTASAVIKQ--GGKSWFFLVADYAFGHSLANDTSAVVKASGGTVVGSIKHPPQ 208 T + T ++++ K W+ + Y FG L + + K G VG+ H Sbjct: 145 PTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLT 204 Query: 209 SSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVTKTMK-LVGLLMVINDIHAL 267 + S +L A A+K +L L N G + ++++ A FG+ L+ + L Sbjct: 205 EKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETL 264 Query: 268 GLANAEGLMMTDSWYWDKDEP-SRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIG 326 G +G+ ++ D P +++F + +K P+ A +Y T L A+ Sbjct: 265 GADICDGVYFGAQYWHAIDAPFNKEFVKLVNDNLKIDPNYSLAGSYICTKIILDAIIKAN 324 Query: 327 TDDSTKVMAQLKKTRIDDFYNKGSIR-ADGRNIHDMYLYQVKSPAESKKPWDYLKLLETI 385 + D V+A ++ + + IR D + I + YL + K+ A+ DY ++ + Sbjct: 325 STDPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYADIVSS- 383 Query: 386 PGEQAFTSVADSKCSL 401 ++F + ++C L Sbjct: 384 --GKSFLPLEKTQCKL 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,182,796 Number of extensions: 47710 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 12 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)