BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0221.p (469 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312337|ref|YP_003776429.1| transcription regulator protein... 957 0.0 gi|300311134|ref|YP_003775226.1| transcription regulator protein... 318 7e-88 gi|300313928|ref|YP_003778020.1| GntR family transcription regul... 308 6e-85 gi|300312133|ref|YP_003776225.1| transcription regulator protein... 256 4e-69 gi|300311016|ref|YP_003775108.1| MocR family transcription regul... 137 3e-33 >gi|300312337|ref|YP_003776429.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 469 Score = 957 bits (2475), Expect = 0.0, Method: Compositional matrix adjust. Identities = 466/469 (99%), Positives = 468/469 (99%) Query: 1 MAEASTTLTSTLVQLIRRQIAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGL 60 MAEASTTLTSTLVQLIRRQIAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGL Sbjct: 1 MAEASTTLTSTLVQLIRRQIAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGL 60 Query: 61 AEPRRGAGFFVCVAPPRPKEDEEGNLSRAMDVVWLMREQLKHDPGQLRLGDGFPPMEWLH 120 AEPRRGAGFFVCVAPPRPKEDEEGNLSRAMDVVWLMREQLKHDPGQLRLGDGFPP+EWLH Sbjct: 61 AEPRRGAGFFVCVAPPRPKEDEEGNLSRAMDVVWLMREQLKHDPGQLRLGDGFPPVEWLH 120 Query: 121 DVRLDKFHQKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHGANQA 180 DVRLDKFHQKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHGANQA Sbjct: 121 DVRLDKFHQKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHGANQA 180 Query: 181 LDIVIRHFIRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLERELQKPGK 240 LDIVIRHFIRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLEREL +PGK Sbjct: 181 LDIVIRHFIRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLERELHRPGK 240 Query: 241 PPLFFTQSLAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPATAPRIATLDQL 300 PPLFFTQSLAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPATAPRIATLDQL Sbjct: 241 PPLFFTQSLAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPATAPRIATLDQL 300 Query: 301 RRTVYVGSFSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLDVILSDGHF 360 RRTVYVGSFSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLDVILSDGHF Sbjct: 301 RRTVYVGSFSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLDVILSDGHF 360 Query: 361 SRHTARLRERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFEDAKRLAQRLMQRQ 420 SRHTARLRERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFEDAKRLAQRLMQRQ Sbjct: 361 SRHTARLRERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFEDAKRLAQRLMQRQ 420 Query: 421 IVLAPGSIFHVDTEQTQPWSRFNVGLLADARFADAMMDIRAAQERQATR 469 IVLAPGSIFHVDTEQTQPWSRFNVGLLADARFADAMMDIRAAQERQATR Sbjct: 421 IVLAPGSIFHVDTEQTQPWSRFNVGLLADARFADAMMDIRAAQERQATR 469 >gi|300311134|ref|YP_003775226.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 500 Score = 318 bits (815), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 180/457 (39%), Positives = 262/457 (57%), Gaps = 14/457 (3%) Query: 3 EASTTLTSTLVQLIRRQIAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGLAE 62 E+ +L +V+ IR +I + + G R+PSIR+ A + ++ T+V +D L A G E Sbjct: 35 ESGDSLVEQIVRGIRARIDDKLLRSGMRMPSIRQFADTNQISRFTVVEAYDRLVAQGYLE 94 Query: 63 PRRGAGFFVCVAPP------RPKEDEEGNLSRAMDVVWLMREQLKHDPGQLRLGDGFPPM 116 RRG+GF+V P + + + R +DVVWL+R + P G G P Sbjct: 95 SRRGSGFYVKERAPLGGSYGAAQAELDTEPGRGLDVVWLVRNMFRQMPPAKMPGGGLLPP 154 Query: 117 EWLHDVRLDKFHQKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHG 176 +WL + + V R L GYG+ G+LPLRQ L KLA+ I A QIVLT G Sbjct: 155 DWLDGELIANALRAVSRDNPQLLLGYGTPQGFLPLRQQLQLKLAELEIAAAPEQIVLTTG 214 Query: 177 ANQALDIVIRHFIRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLERELQ 236 Q LD V RHF+RPGD + VD+P +++++G GA+++GIPRL DGPD+A L REL Sbjct: 215 VTQGLDTVARHFLRPGDVIFVDDPAWFLMFGSFAALGAKVVGIPRLGDGPDIARL-RELA 273 Query: 237 KPGKPPLFFTQSLAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPAT----AP 292 KP L+ S+ HNPT++ S KA ++L++A+ + +IVE+D + D P + A Sbjct: 274 AVHKPKLYVINSVLHNPTSTSLSAAKAFQVLRIAEEHDFVIVEDDIYCDMHPGSAVQPAT 333 Query: 293 RIATLDQLRRTVYVGSFSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLD 352 RIA LDQL+R +Y+G FSK++SA LRVG+IA +LA LAD K+L +++ E ER Sbjct: 334 RIAALDQLQRVIYLGGFSKTLSANLRVGYIATSAELARSLADRKLLSTLTTGEIGERVAY 393 Query: 353 VILSDGHFSRHTARLRERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFEDAKRL 412 +LS+GH+ +H R+R RL A ++ QLE LG ++ AP M+LW D + Sbjct: 394 KVLSEGHYRKHAERVRARLNGARDKVMRQLERLGMDVVEAPPCGMFLWVDT--GRDTNVM 451 Query: 413 AQRLMQRQIVLAPGSIFHVDTEQTQPWSRFNVGLLAD 449 + MQ +LAPGS+F + + R NV +AD Sbjct: 452 TEAAMQEGFLLAPGSLFS-PAQLPSTYMRINVASMAD 487 >gi|300313928|ref|YP_003778020.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 472 Score = 308 bits (789), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 169/438 (38%), Positives = 244/438 (55%), Gaps = 24/438 (5%) Query: 20 IAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGLAEPRRGAGFFVCV------ 73 I G G RLPS+R+ A H +T+V +++L A G+ + RRGAGFFV Sbjct: 33 IETGELRSGERLPSVRQFAQHHDIGSSTVVEAYEQLVARGMLQARRGAGFFVAARAGQSG 92 Query: 74 -----APPRPKEDEEGNLSRAMDVVWLMREQLKHDPGQLRLGDGFPPMEWLHDVRLDKFH 128 PP P +D WL+ E + ++ G G+ P +WL+D L + Sbjct: 93 QHAVFVPPEP----------VIDSAWLLSEMFADERVPIKAGCGWLPGKWLNDEGLHQAE 142 Query: 129 QKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHGANQALDIVIRHF 188 ++++R+ GYG +GY PLRQ + L +E G Q++ THGA QALD+VIR Sbjct: 143 RRIIRSPGAQQVGYGHPYGYAPLRQLITQFLGHWSLELGIEQVLTTHGATQALDLVIRTM 202 Query: 189 IRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLERELQKPGKPPLFFTQS 248 +PGD VL+D+PGY L L+L+G +IG+PR P G D VLE L + P +FFT S Sbjct: 203 TQPGDTVLIDDPGYCNLIAMLRLAGLNVIGVPRTPGGVDTDVLE-TLARTHHPKMFFTTS 261 Query: 249 LAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPATAPRIATLDQLRRTVYVGS 308 + NPT + +P A R+LQ A+R+ +VE+D F + AT P +A LD L+R +YVGS Sbjct: 262 VLQNPTGTSYTPACAMRVLQAAERHGFWVVEDDIFRELGQATDPMLAALDGLQRVIYVGS 321 Query: 309 FSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLDVILSDGHFSRHTARLR 368 +SK+++ +LRVG+IAC R+LA + KM++ +++SE ER + +L++GH RH L Sbjct: 322 YSKTIAPSLRVGYIACQRELARQIVHTKMVLTLTNSEINERLVHNVLTEGHHRRHVQTLN 381 Query: 369 ERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFE-DAKRLAQRLMQRQIVLAPGS 427 L A A+L +G F A + M+ WA E A+ +A R Q+ I LAPG Sbjct: 382 ATLLSAQATLNAKLSEVGLTPFTAARGGMFSWATIEGCELSAREIADRARQKGIWLAPGD 441 Query: 428 IFHVDTEQTQPWSRFNVG 445 FH+ Q RFNVG Sbjct: 442 FFHLSAPH-QTSFRFNVG 458 >gi|300312133|ref|YP_003776225.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 256 bits (653), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 163/447 (36%), Positives = 243/447 (54%), Gaps = 22/447 (4%) Query: 12 LVQLIRRQIAEGV----YAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGLAEPRRGA 67 LV I + EGV A G +LPS+R +A +++T+ +++L A G R GA Sbjct: 32 LVDRIFTAVLEGVRTRRLAHGDKLPSLRVVAESAQVSRDTVARAYEKLVAHGHVYTRPGA 91 Query: 68 GFFVCVAPPRPKEDEEGNLSRAMDVVWLMRE--QLKHDPGQL---RLGDGFPPMEWLHDV 122 GFFV V +++ N L+ Q DP L LG G P EW++ Sbjct: 92 GFFVRV------DNQVANAQTQTAPAPLLTNWRQRLLDPRTLLHRGLGSGDLPAEWINVP 145 Query: 123 RLDKFHQKVVRTGIGALFGYGSRFGYLPLRQHLVHKLAQHGIEAGTGQIVLTHGANQALD 182 +L + V R+ I GYG GYLPLRQ L KLA G++ Q++ T GA A++ Sbjct: 146 QLAAALRAVSRSNINWAAGYGDAQGYLPLRQQLQRKLADIGVDVTPPQVLTTLGATDAIN 205 Query: 183 IVIRHFIR-PGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPDGPDVAVLERELQKPGKP 241 +++ ++R PG VLVD+PG ++L +L SG I+G+ R DGPD+ L+ ++ G P Sbjct: 206 LIVMSYLRQPGMTVLVDDPGSFLLMERLLASGMHIVGVARDADGPDITALQNACEQHG-P 264 Query: 242 PLFFTQSLAHNPTASDTSPHKAHRILQLADRYQAIIVENDAFADFKPAT----APRIATL 297 +F QSL H+P + SP +A +IL+LA+R+ IVE+D + D PA A R+A+L Sbjct: 265 TFYFCQSLLHSPGYTGLSPQRAFQILRLAERFNFTIVEDDTYGDLAPAHRSPHATRLASL 324 Query: 298 DQLRRTVYVGSFSKSVSAALRVGFIACHRDLASDLADIKMLVHMSSSEYCERTLDVILSD 357 DQL+ +YVGSFSK++ A +R GFIA + L K++ S+ ER + +LS+ Sbjct: 325 DQLQHVLYVGSFSKTLGAGMRAGFIAGNPQQIEWLLLYKLVSRASNGSLAERAIYRLLSE 384 Query: 358 GHFSRHTARLRERLAQATEQARAQLEGLGAELFCAPQQAMYLWARFPWFEDAKRLAQRLM 417 G + RH +LR RL QA + A LE LG L P +YLW + P +A +A+R++ Sbjct: 385 GGYRRHCEQLRTRLDQARPRLMAALEALGIVLPHRPDAGLYLWGKLPGDLNAMEVAERML 444 Query: 418 QRQIVLAPGSIFHVDTEQTQPWSRFNV 444 + APG +F + + + RFNV Sbjct: 445 ANGYLTAPGPVFS-SGQAARSYMRFNV 470 >gi|300311016|ref|YP_003775108.1| MocR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 480 Score = 137 bits (344), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 119/457 (26%), Positives = 209/457 (45%), Gaps = 47/457 (10%) Query: 4 ASTTLTSTLVQLIRRQIAEGVYAPGSRLPSIREMAARHGCAKNTIVNVFDELTALGLAEP 63 A T L + I +GV PG R+ S+R + +H + T+V ++ L + G+ E Sbjct: 2 AVTPLYKQFADQLAASIRDGVLRPGERIASVRIASQQHKVSVTTVVRAYELLESRGIIES 61 Query: 64 RRGAGFFV---CVAPPRP------KEDEEG-NLSRAMDVVWLMREQLK--HDPGQLRLGD 111 + +G+FV APP+P +ED S +DV L+ LK G + LG Sbjct: 62 QPQSGYFVREQPEAPPKPALRTTSREDHPAWQESTEVDVSRLVLATLKTIQQDGTVPLGS 121 Query: 112 GFPPMEWLHDVRLDKFHQKVVRTGIGALFGYGSRFGYLP-----LRQHLVHKLAQHGIEA 166 +P RL ++ ++ R S F LP L + + + ++G++ Sbjct: 122 PYPNPALFPSHRLAQYAGRIARHA-----SQSSVFDDLPPGHPELLRQIARRYLENGLKV 176 Query: 167 GTGQIVLTHGANQALDIVIRHFIRPGDRVLVDEPGYYMLYGKLKLSGARIIGIPRLPD-G 225 +IV+T GA +A+++ ++ RPGD + V+ P +Y + ++ G R + IP P G Sbjct: 177 DPEEIVITVGATEAINLCLQAVARPGDTIAVETPTFYAMLHAIERLGMRALEIPTDPQRG 236 Query: 226 PDVAVLERELQKPGKPPLFFTQSLA--------HNPTASDTSPHKAHRILQLADRYQAII 277 DV LE ++ TQ++A NP + R+++LA+++Q + Sbjct: 237 IDVGALEERMK---------TQAIAACMVMPNFQNPLGFQMPDSEKERLVRLAEQHQMPV 287 Query: 278 VENDAFAD--FKPATAPRIATLDQLRRTVYVGSFSKSVSAALRVGFIACHRDLASDLADI 335 +EN +++ + A + D+ ++ SFSKS+++A R+G+ R A + + Sbjct: 288 IENAVYSELYYGNAHPSSLKNYDRDGLVLHCSSFSKSLTSAYRIGWAMPGRYRA-QVEKL 346 Query: 336 KMLVHMSSSEYCERTLDVILSDGHFSRHTARLRERLAQATEQAR---AQLEGLGAELFCA 392 K L +++ +R + L+ G + RH R+R AQ + R + G ++ Sbjct: 347 KFLNTLATPTAPQRAIAEYLTQGGYERHLRRVRRAFAQQRDMMRHFVLRFFPAGTQV-SE 405 Query: 393 PQQAMYLWARFPWFEDAKRLAQRLMQRQIVLAPGSIF 429 PQ LW P DA L +R + I +APG IF Sbjct: 406 PQGGYVLWVELPQGVDAMELYRRSLALGITVAPGRIF 442 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,558,216 Number of extensions: 70137 Number of successful extensions: 242 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 11 Length of query: 469 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 380 Effective length of database: 1,198,455 Effective search space: 455412900 Effective search space used: 455412900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)