BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0220.p (536 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 1066 0.0 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 546 e-156 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 513 e-146 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 507 e-145 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 444 e-125 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 1066 bits (2758), Expect = 0.0, Method: Compositional matrix adjust. Identities = 534/536 (99%), Positives = 535/536 (99%) Query: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 Query: 181 EIDAIAAKSRTLILVLEGLILLLGVVFARYLTLGITKPLHRAVDVSRRVAEGDLSSEIAV 240 EIDAIAAKSRTLILVLEGLILLLG+VFARYLTLGITKPLHRAVDVSR VAEGDLSSEIAV Sbjct: 181 EIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAV 240 Query: 241 TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLA 300 TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLA Sbjct: 241 TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLA 300 Query: 301 ETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVD 360 ETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVD Sbjct: 301 ETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELIND 420 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELIND Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELIND 420 Query: 421 SVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQV 480 SVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQV Sbjct: 421 SVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQV 480 Query: 481 TQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLVRREIDVTPSSPVLPQ 536 TQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLVRREIDVTPSSPVLPQ Sbjct: 481 TQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLVRREIDVTPSSPVLPQ 536 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 546 bits (1406), Expect = e-156, Method: Compositional matrix adjust. Identities = 329/540 (60%), Positives = 405/540 (75%), Gaps = 6/540 (1%) Query: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 M NM IGRRL LGFA+IL + ++ + +WRL +A AT EMM EPL ERL++DWY Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 + +RRT AIAKSSDP+L +FA + AA+S+ S E QK +EAL+ + +EKA+F K+G Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 E+RK Y+ RD I+KAKA G TEEA +IL F Y D +++L+ QR +D A Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 Query: 181 EIDAIAAKSRTLILVLEGLILLLGVVFARYLTLGITKPLHRAVDVSRRVAEGDLSSEI-- 238 I + +SR L+L L L ++LG +FAR L LGIT+PL +AV+V+ VA GDL+ I Sbjct: 181 HIQTVYRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE 240 Query: 239 AVTSR-DETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQAS 297 + +R DETG+L+ +L M +L IV VR GTD I+TAS EIA GNLDLS+RTE+QA Sbjct: 241 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG 300 Query: 298 SLAETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRK 357 +L ETASAMEEL STV QNADNARQA+QLA SAS+VA+QGG VVSQV+ TMG+IN SSRK Sbjct: 301 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK 360 Query: 358 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKEL 417 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIK L Sbjct: 361 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL 420 Query: 418 INDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQM 477 I+DSV KV GS LV +AG+TM EVV+SVR+V DIV+EISAAS+EQ+ GI Q+NLAIT M Sbjct: 421 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM 480 Query: 478 DQVTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLV---RREIDVTPSSPVL 534 DQ TQQNAALVEEAAAAA +MQ QA RL ++VS F+L ++ + R +D+TP P L Sbjct: 481 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL 540 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 513 bits (1321), Expect = e-146, Method: Compositional matrix adjust. Identities = 292/511 (57%), Positives = 361/511 (70%) Query: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVA 65 N+ IG RL +GF +L ++ +G+WRL + AT +M LK ER W+ + Sbjct: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 Query: 66 GIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGELRKL 125 RT A+ KS D A YF ++ A S + QK+VEA ++ EEK LF ++G+LR Sbjct: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAA 122 Query: 126 YLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAREIDAI 185 Y ++R I K AG+ A + D V Y D + + DFQ++ +D TA I+ Sbjct: 123 YRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRD 182 Query: 186 AAKSRTLILVLEGLILLLGVVFARYLTLGITKPLHRAVDVSRRVAEGDLSSEIAVTSRDE 245 R+++L L + ++LG A LT IT+PL++AV ++ VA GDL+S I +SRDE Sbjct: 183 YRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDE 242 Query: 246 TGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAETASA 305 TG+LL +LK MN +L IV VR GT+TI+TAS EIA GNLDLS+RTE QA SL ETASA Sbjct: 243 TGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETASA 302 Query: 306 MEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVDIISVI 365 ME+L STV+QNADNARQA++LA SAS VA QGG VV QVV TMGAI+ASS+KI DII VI Sbjct: 303 MEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVI 362 Query: 366 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELINDSVGKV 425 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIK+LI+DSV KV Sbjct: 363 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKV 422 Query: 426 DDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQVTQQNA 485 DDGSRLV +AG+TMQEVV SV RVT +V EI+ AS EQ+ GIEQ+NLAIT +D+ TQQNA Sbjct: 423 DDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNA 482 Query: 486 ALVEEAAAAAASMQTQAGRLMQMVSVFRLSE 516 ALVE+AAAAA SMQ QA L VSVFRL + Sbjct: 483 ALVEQAAAAARSMQDQAASLTDAVSVFRLED 513 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 507 bits (1306), Expect = e-145, Method: Compositional matrix adjust. Identities = 286/512 (55%), Positives = 373/512 (72%) Query: 3 IIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTN 62 +I ++ IG RL GFA++L FS+++A +G+WRL VA T++MM PLKTERL++ W T Sbjct: 1 MIKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTL 60 Query: 63 LVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGEL 122 L+ I+RT + KS DP L + A E AASSK S + K VE LL++ EEK +F I Sbjct: 61 LLIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 123 RKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAREI 182 RK +L+ RD+I AK G+ + + + +V A + QD MR L+DF+R +D + +I Sbjct: 121 RKQFLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQI 180 Query: 183 DAIAAKSRTLILVLEGLILLLGVVFARYLTLGITKPLHRAVDVSRRVAEGDLSSEIAVTS 242 A+ S+ I++LE +ILL G+ FA LT IT+P+H A+ +S+RVA GDL+S Sbjct: 181 QQDASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSRAQAMG 240 Query: 243 RDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAET 302 RDE GQL+ SL+ M++ L G+VS VR G D+I+ AS EIA GNLDLS+RTE+QA +L +T Sbjct: 241 RDELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGALEQT 300 Query: 303 ASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVDII 362 A+++EEL S+VR N+DNA QA++L ++A++VA QGG V+ Q++ TM IN SSRKIVDII Sbjct: 301 AASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRKIVDII 360 Query: 363 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELINDSV 422 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA+AAKEIK+LI+DSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLIDDSV 420 Query: 423 GKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQVTQ 482 GKV GS LVEQAG TMQEVV SV+ +V EIS AS +Q GI Q+N A+ QMD VTQ Sbjct: 421 GKVSAGSTLVEQAGKTMQEVVGSVQGAHTLVGEISTASRQQAEGIHQVNQAVGQMDGVTQ 480 Query: 483 QNAALVEEAAAAAASMQTQAGRLMQMVSVFRL 514 QNAALVE+AAAAA S++ QA +L + V+ F L Sbjct: 481 QNAALVEQAAAAAKSLEDQAAQLKRAVAFFSL 512 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 444 bits (1141), Expect = e-125, Method: Compositional matrix adjust. Identities = 282/517 (54%), Positives = 359/517 (69%), Gaps = 4/517 (0%) Query: 4 IGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNL 63 + ++ I RL + FA+++ +I G+G+ L +++++ ++ + + L + N+ Sbjct: 3 LKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNV 62 Query: 64 VAGIR--RTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGE 121 R R + ++ + A + AA+S + E ++E +LST + LFQ I E Sbjct: 63 NGAARNLRNALLGRNLEEA--TRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQE 120 Query: 122 LRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTARE 181 R Y + RD++ + EEA+ +L +P Y + + V FQ+ M+ + E Sbjct: 121 ARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTE 180 Query: 182 IDAIAAKSRTLILVLEGLILLLGVVFARYLTLGITKPLHRAVDVSRRVAEGDLSSEIAVT 241 + ++L L + + L V+ A +T IT+PL+ AV V+ VA GDL+ +I T Sbjct: 181 GRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGAT 240 Query: 242 SRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAE 301 S+DETG LL +LK MN SL IVS VR G+DTI+TAS+EIA GNLDLSSRTE+QA +L E Sbjct: 241 SKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEE 300 Query: 302 TASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVDI 361 TASAMEEL STV+QNADNARQA+ LA +AS VA QGG VV QVV TMG IN +SRKIVDI Sbjct: 301 TASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDI 360 Query: 362 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELINDS 421 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIK LI+DS Sbjct: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDS 420 Query: 422 VGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQVT 481 V +VD+GSRLVEQAG TM EVV+SVRRVTD+VAEISAAS EQ+ GIEQIN AITQMDQVT Sbjct: 421 VSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVT 480 Query: 482 QQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSERE 518 QQNAALVEEAAAAA S+Q Q+ RL VSVF+LS + Sbjct: 481 QQNAALVEEAAAAAQSLQEQSTRLSATVSVFKLSSHD 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.327 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,222,682 Number of extensions: 40651 Number of successful extensions: 551 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 71 Length of query: 536 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 446 Effective length of database: 1,193,720 Effective search space: 532399120 Effective search space used: 532399120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)