BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0209.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis pro... 1097 0.0 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 136 5e-33 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 135 9e-33 gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I p... 133 3e-32 gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 132 1e-31 >gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 1097 bits (2836), Expect = 0.0, Method: Compositional matrix adjust. Identities = 541/541 (100%), Positives = 541/541 (100%) Query: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW Sbjct: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 Query: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET 120 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET Sbjct: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET 120 Query: 121 RQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRIN 180 RQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRIN Sbjct: 121 RQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRIN 180 Query: 181 DAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRRVALDRN 240 DAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRRVALDRN Sbjct: 181 DAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRRVALDRN 240 Query: 241 DEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 DEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG Sbjct: 241 DEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 Query: 301 ATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQNVMDAAGSVSAKLA 360 ATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQNVMDAAGSVSAKLA Sbjct: 301 ATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQNVMDAAGSVSAKLA 360 Query: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV 420 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV Sbjct: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV 420 Query: 421 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQLSQIIAQVQALAPRFQI 480 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQLSQIIAQVQALAPRFQI Sbjct: 421 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQLSQIIAQVQALAPRFQI 480 Query: 481 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELHVVSNELRNGVSRFKVQ 540 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELHVVSNELRNGVSRFKVQ Sbjct: 481 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELHVVSNELRNGVSRFKVQ 540 Query: 541 D 541 D Sbjct: 541 D 541 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 136 bits (342), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 145/554 (26%), Positives = 256/554 (46%), Gaps = 58/554 (10%) Query: 3 NWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAWYE 62 N I +R+ FALIL ++AGI L R+++ A A R+ L ++ A WY Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGI---GLWRLDHVAQATREMMAEPLKTERLM-ADWYT 61 Query: 63 NLL--MSQQIAQIDTNEAERQADLAL-----LRRNGERLDRLQEDYDKTIFSDSDRKLFD 115 NL+ + + IA +++ A + +GE Q+ + + + ++ LF Sbjct: 62 NLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGE----YQKQVEALLSTPEEKALFQ 117 Query: 116 QFKETRQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQR-MVDTNKSIADD 174 + E R+ Y + E+ K + +Q+ YQ T R +VD + DD Sbjct: 118 KIGELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDD 177 Query: 175 AASRINDAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRR 234 A I+ ++ +L LL +V +L I +PL + + + + GD S Sbjct: 178 TAREIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSE 237 Query: 235 VALDRNDEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAAT-------ARQQQA 287 +A+ DE G L M L +VS + + ++T+ +EIAA QQ + Sbjct: 238 IAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQAS 297 Query: 288 TAAETAATTTEIGATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQN 347 + AETA+ E+ +T R+ +R + N S V+EQ G G + Sbjct: 298 SLAETASAMEELISTVRQNADNARQASQLANSASAVAEQ-----GGG---------VVSQ 343 Query: 348 VMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVV 407 V+D G++ N + I +++ I +A QTN+L+LNAA+EA +AGE GRGF+VV Sbjct: 344 VVDTMGAI-------NASSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV 396 Query: 408 ATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQLSQI 467 A+E+R LA ++A A +I+ ++ + V G + +Q MQ+V ++ +++ I Sbjct: 397 ASEVRTLAQRSASAAKEIKELINDSVGKVDDG----SRLVEQAGNTMQEVVSSVRRVTDI 452 Query: 468 IAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELHVVS 527 +A++ A + Q + EQIN A+ Q+ + QQ +++++ A + + Sbjct: 453 VAEISAASTE----------QTHGIEQINLAITQMDQVTQQNAALVEEAAAAAASMQTQA 502 Query: 528 NELRNGVSRFKVQD 541 L VS F++ + Sbjct: 503 GRLMQMVSVFRLSE 516 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 135 bits (340), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 141/559 (25%), Positives = 257/559 (45%), Gaps = 68/559 (12%) Query: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 +K+ I R+ FA+++ +M L+ G+ +L I + + +D Sbjct: 3 LKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDR-------------- 48 Query: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGER----LDRLQEDYDKT---------IFS 107 YE + ++ +I + + N A R ALL RN E LDR + KT I S Sbjct: 49 YEKIALAVEI-RDNVNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILS 107 Query: 108 DS-DRKLFDQFKETRQKY---RAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQR 163 + R+LF +E R Y R + E++K + A ++ +F Q+ P + +Q+ + Sbjct: 108 TARGRELFQSIQEARSAYNTPRDKLRELIKQQKKEEA-VELLFT-QVIPAQDKYFQVLDQ 165 Query: 164 MVDTNKSIADDAASRINDAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGIL 223 V KS+ + + + D SA ML A+ ++ + + R+I PL + + + Sbjct: 166 FVAFQKSLMEQSVTEGRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVA 225 Query: 224 DYMRSGDFSRRVALDRNDEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATAR 283 + +GD + ++ DE G L + M D L +VS+ ++ S + T+ EIA Sbjct: 226 SAVAAGDLTVQIGATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNL 285 Query: 284 QQQATAAETAATTTEIGATSREIHSTSR---DLVRTMNEVSVVSEQTSALAGNGQAGLTR 340 + E A E + E+ ST + D R N ++ + + + G+ Sbjct: 286 DLSSRTEEQAGALEETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGS------- 338 Query: 341 MEDTMQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEH 400 G V + +N+ + I +++ I +A QTN+L+LNAA+EA +AGE Sbjct: 339 ----------VVGQVVQTMGEINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQ 388 Query: 401 GRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTA 460 GRGF+VVA+E+R LA ++A A +I+ ++ D +V G + V+ A Sbjct: 389 GRGFAVVASEVRTLAQRSASAAKEIKALI--------------DDSVSRVDNGSRLVEQA 434 Query: 461 GSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAI 520 G+ +++++A V+ + ++ Q++ EQIN A+ Q+ + QQ +++++ A Sbjct: 435 GTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVTQQNAALVEEAAAAA 494 Query: 521 EELHVVSNELRNGVSRFKV 539 + L S L VS FK+ Sbjct: 495 QSLQEQSTRLSATVSVFKL 513 >gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 133 bits (335), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 132/524 (25%), Positives = 254/524 (48%), Gaps = 59/524 (11%) Query: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 KN I +++ +F +L+LMA++ S L +++N ++ + D P + + Sbjct: 4 FKNLKIARKLQLAFLAVLVLMAILGIFSISQLAKVKNASSEISTDWMPA--QKKLGDLKL 61 Query: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNG---ERLDRLQEDYDKTIFSDSDRKLF--- 114 Y L + +Q+ + + +A L+ R+ LD+ +DY+ ++ + +R+L+ Sbjct: 62 YFVRLRGTE-SQLAIYDGDVEAMNGLVSRSQAILTGLDKTMKDYEASLDNVPERELYTKV 120 Query: 115 -DQFKETRQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIAD 173 Q E +++ +++ L D Q + ++ ++ PQ + +++ N+ + Sbjct: 121 AGQIGEFLAEHKKIVAAFLARDNPQGRDLFLGYSNKLYPQILGDLE---KLIKINQDGGN 177 Query: 174 DAASRINDAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSR 233 A + +D + ++ +L L AAL + ++ R I PLQ + I + GD Sbjct: 178 AATADADDTFSKSRVGVLVLLAAALAIGMGLALYVARIIARPLQDAVDIARRVAEGDLGA 237 Query: 234 RVALDRNDEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIA------ATARQQQA 287 ++ + DE G L D M + L +V Q + + +AT+ TEIA + +QQA Sbjct: 238 QIQVQSKDETGQLMQALDTMNEGLRKVVGQVRSGTDSIATASTEIATGNLDLSRRTEQQA 297 Query: 288 TAAETAATTTEIGATSREIHSTSR---DLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDT 344 TA E A+ E E+ ST + D R N++++ + S +A G + ++ +T Sbjct: 298 TALEETASALE------ELTSTVKQNTDNARQANQLAMAA---SGVAARGGGVVGQVVET 348 Query: 345 MQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGF 404 M+ + NE + I ++++ I +A QTN+L+LNAA+EA +AGE GRGF Sbjct: 349 MEAI--------------NEASNRIVEIISVIDGIAFQTNILALNAAVEAARAGEQGRGF 394 Query: 405 SVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQL 464 +VVA+E+R LA ++A A +I+ ++ + S V AG V+ AGS + Sbjct: 395 AVVASEVRSLAQRSASAAKEIKQLIDDSVSKVGAGT--------------SLVRQAGSTM 440 Query: 465 SQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQ 508 S+++ V+ + ++ Q+ ++IN+A+ Q+ E QQ Sbjct: 441 SEVVESVKRVTDVVSEISAASTEQSVGIDEINRAVTQMDEVTQQ 484 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 132 bits (331), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 137/534 (25%), Positives = 253/534 (47%), Gaps = 76/534 (14%) Query: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATAL------RQDSTPGLYYST 54 MKN I R+ FA +L+L+ +A + R+ + L + ++ + ++ Sbjct: 1 MKNLKIGHRLGLGFAAVLILLVAVAAFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 Query: 55 MINAAWYENLLMSQQIAQIDTNEAERQADLALLRRNGERLD----RLQEDYDKTIFSDSD 110 + A NL + Q + A+++ DL L+ + + +D L S + Sbjct: 61 GLRAIAARNLTLVQ-------SAADQKNDLDLVGSSQQDIDAGMAELGRLMQHPASSPQE 113 Query: 111 RKLFDQFKETRQKYRAMISEVLKMDFSQ-----RAPIDK----MFNEQIRPQWRQGYQLT 161 R++ ++ ++ +Y + ++K+ SQ RA + K + N I Q Q +L Sbjct: 114 REMLEKLRQLEAQYLPVAVNIVKLATSQQTEAARASLVKDCMPILNRVI-AQVEQFNKLL 172 Query: 162 QRMVDTNKSIADDAASRINDAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMG 221 ++ ++ A T A++ ML++ +AAL + + L R+I+ PL + + Sbjct: 173 RKN-------SEANVEEAEAAYTLAKWTMLAASLAALTLGGMVAWLLTRSISRPLAQAVS 225 Query: 222 ILDYMRSGDFSRRVALDRNDEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEI--- 278 + ++SGD R+ + DE G L S M D L +V + ++ + +A + EI Sbjct: 226 VAQSVQSGDLGSRIEVTSKDELGQLMSALKGMNDSLVNIVGEVRQGTDTIAVASDEIKQG 285 Query: 279 ----AATARQQQATAAETAATTTEIGATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNG 334 + QQ + ETA+ ++ AT ++ +R+ R +++ S +A G Sbjct: 286 NMDLSNRTEQQAGSLQETASAMEQLTATVKQNADNAREANR-------LADNASQVAARG 338 Query: 335 QAGLTRMEDTMQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEA 394 A + R+ DTM S+SA + I +++ I +A QTN+L+LNAA+EA Sbjct: 339 GAAVGRVVDTMN-------SISA-------SSHKIVDIISVIDGIAFQTNILALNAAVEA 384 Query: 395 EKAGEHGRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGM 454 +AGE GRGF+VVA+E+R LA ++AVA +IE ++ E +VA V G + S+ Sbjct: 385 ARAGEQGRGFAVVASEVRTLAQRSAVAAKEIEALINE---SVAKVVTGEKQVSE------ 435 Query: 455 QDVQTAGSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQ 508 AGS + +++ ++++ ++ Q + EQIN+A+ Q+ EA QQ Sbjct: 436 -----AGSTMHEVVESIKSVTHIMHDISAASAEQTSGLEQINRAVAQIDEATQQ 484 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.126 0.332 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,202,393 Number of extensions: 40923 Number of successful extensions: 541 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 70 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)