BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0207.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 855 0.0 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 91 2e-19 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 84 3e-17 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 80 3e-16 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 58 2e-09 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 433/433 (100%), Positives = 433/433 (100%) Query: 1 MKSLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQAL 60 MKSLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQAL Sbjct: 1 MKSLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQAL 60 Query: 61 IELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA 120 IELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA Sbjct: 61 IELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA 120 Query: 121 GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQGQWRLHERIRSQ 180 GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQGQWRLHERIRSQ Sbjct: 121 GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQGQWRLHERIRSQ 180 Query: 181 VRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAE 240 VRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAE Sbjct: 181 VRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAE 240 Query: 241 GGILLGPTMESAGIALAFGFRKRVAAAVPPLPPAWASLVQAAPPAPTPVRPDAAHRAPAR 300 GGILLGPTMESAGIALAFGFRKRVAAAVPPLPPAWASLVQAAPPAPTPVRPDAAHRAPAR Sbjct: 241 GGILLGPTMESAGIALAFGFRKRVAAAVPPLPPAWASLVQAAPPAPTPVRPDAAHRAPAR 300 Query: 301 RIVASAALGAVGTATAARPASPATALPAGLLERARALADQGRFAQAGELCEQAVQQQGPS 360 RIVASAALGAVGTATAARPASPATALPAGLLERARALADQGRFAQAGELCEQAVQQQGPS Sbjct: 301 RIVASAALGAVGTATAARPASPATALPAGLLERARALADQGRFAQAGELCEQAVQQQGPS 360 Query: 361 AEAFYLQGLIHDAGGQQELAQRCYRKALYLEPEHQAALLQLAALLQAQGDASGAQRMRQR 420 AEAFYLQGLIHDAGGQQELAQRCYRKALYLEPEHQAALLQLAALLQAQGDASGAQRMRQR Sbjct: 361 AEAFYLQGLIHDAGGQQELAQRCYRKALYLEPEHQAALLQLAALLQAQGDASGAQRMRQR 420 Query: 421 AERMAKPGEGAHG 433 AERMAKPGEGAHG Sbjct: 421 AERMAKPGEGAHG 433 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 84/254 (33%), Positives = 127/254 (50%), Gaps = 16/254 (6%) Query: 11 LKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAA--ELQALIELAVVPE 68 K +GL + +L++ + R+ L + Y +L+ S A E Q I+L E Sbjct: 25 FKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQERQRAIDLITTNE 84 Query: 69 TWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDAGLTPGQFV 128 T+FFR+ V +LA QP +RI S +TGEE Y+LAM LLD + Sbjct: 85 TYFFREPGHFDFVVGQWLPQLAQQPQ--LRIWSAASATGEEAYTLAM-LLDHHRPGASWS 141 Query: 129 IDAYDIRTRSLEIAAAGLYGRNSFRGQEL--AFRDRHFDLEQGQWRLH----ERIRSQVR 182 + A DI TR L A LY RGQ + A+ R+ + G++ H +R++V+ Sbjct: 142 VFASDISTRVLRFARRALY--PMVRGQNIPRAYLRRYCLMGVGEYEGHFLVQADLRAKVQ 199 Query: 183 FAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAEG- 241 FA NL D Q YD IF RNV+IYF+ + + QV++ + + ++P + VG +E Sbjct: 200 FAQRNLTRLDEHDDGQ-YDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVVGHSESL 258 Query: 242 -GILLGPTMESAGI 254 G+ LG M+S I Sbjct: 259 HGMDLGLDMQSPSI 272 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 64/203 (31%), Positives = 98/203 (48%), Gaps = 8/203 (3%) Query: 46 YLSLLLSSA--AELQALIELAVVPETWFFRDREAI--LAVARLASDKLAAQPAAVIRILS 101 Y SLL A ELQ L L V ET+F R+ L + L + A IRI Sbjct: 53 YFSLLRFEADGKELQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWC 112 Query: 102 LPCSTGEEPYSLAMALLDA--GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAF 159 +PCS+GEE Y+LAM L++ G+ I + DI T L A G Y S + + Sbjct: 113 IPCSSGEEAYTLAMYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADW 172 Query: 160 RDRHFDLEQG-QWRLHERIRSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQR 218 ++F L QG ++++ + +R V F NL + + +D IFCRN+LIYF+ ++ Sbjct: 173 MSKYF-LRQGDEYQICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRK 231 Query: 219 QVVQLLASVMKPDALIFVGPAEG 241 + + + + I +G +E Sbjct: 232 EAAETFYEALNLNGFICLGHSES 254 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 67/218 (30%), Positives = 104/218 (47%), Gaps = 19/218 (8%) Query: 46 YLSLL--LSSAAELQALIELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLP 103 YL +L + E +A T FFR+ +A K +PA I Sbjct: 65 YLDMLERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFL--KTLKEPAT---IWCSA 119 Query: 104 CSTGEEPYSLAMALLDA--GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELA-FR 160 STGEEPY++AM +A LTP ++ A DI T L + G+Y R ++++ R Sbjct: 120 ASTGEEPYTIAMTACEAYGSLTPPVQIV-ATDIDTNVLATGSNGVYPME--RIEKMSPER 176 Query: 161 DRHFDL-----EQGQWRLHERIRSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERD 215 + F L +G R+ +R + F NLLA + Q +D IFCRNV+IYF++ Sbjct: 177 VKKFFLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQ-FDVIFCRNVMIYFDKP 235 Query: 216 VQRQVVQLLASVMKPDALIFVGPAEGGILLGPTMESAG 253 Q +++ +MKP AL+F G +E + + ++ G Sbjct: 236 TQSKILSRFVPLMKPHALLFAGHSENFLYVSDALKLKG 273 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 69/242 (28%), Positives = 97/242 (40%), Gaps = 45/242 (18%) Query: 23 GASLIERVVRERMAVLEMEDDALYLSLL----LSSAAELQALIELAVVPETWFFRDREAI 78 GASL + R+A + D LS L L AA L+E V + FRD Sbjct: 38 GASL-----KRRLATAMIRFDCQTLSQLQDRVLHDAAVFPLLMEYLTVQVSEMFRDPSYF 92 Query: 79 LAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDAGLTPGQFVIDAYDIRTRS 138 LA+ L P+ ++I CS+GEE YSLA+ L + GL +I A DI + Sbjct: 93 LALREKVVPLLRTYPS--LKIWVAGCSSGEEVYSLAILLREEGLLERS-IIYATDINQSA 149 Query: 139 LEIAAAGLYGRNSFRGQELAFRDRH------------FDLEQGQWRLHERIRSQVRFAPG 186 L+ A AG+Y G F + H + G + +R V F+ Sbjct: 150 LKKAEAGVYDLERIAG----FTNNHQKSGARTSLSDYYTAAYGAVVFDKSLRKNVVFSD- 204 Query: 187 NLLAPDFLRHSQPYDFIF-------CRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPA 239 HS D +F CRNVLIYF R +Q + + L + + +G Sbjct: 205 ---------HSLATDSVFAEMHLVSCRNVLIYFNRQLQERALGLFRESLVRKGFLGLGSK 255 Query: 240 EG 241 E Sbjct: 256 ES 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,059,284 Number of extensions: 38708 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 5 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)