BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0205.p (775 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 1542 0.0 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 195 1e-50 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 188 2e-48 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 186 7e-48 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 168 2e-42 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 1542 bits (3993), Expect = 0.0, Method: Compositional matrix adjust. Identities = 771/775 (99%), Positives = 772/775 (99%) Query: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNASLLALEQQPTAADHLEACMRAGHSLKGAARIV 60 MSTPDLSQMSMLDLFRFEAESQIAQLNASLLALEQQPTAADHLEACMRAGHSLKGAARIV Sbjct: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNASLLALEQQPTAADHLEACMRAGHSLKGAARIV 60 Query: 61 GLEPAVKVAHVLEDCFVLAQQGRLLLQKKHIDVLLRGADLLARIAAPPDGDEAWADHAGS 120 GLEPAVKVAHVLEDCFVLAQQGRLLLQKKHIDVLLRGADLLARIAAPPDGDEAWADHAGS Sbjct: 61 GLEPAVKVAHVLEDCFVLAQQGRLLLQKKHIDVLLRGADLLARIAAPPDGDEAWADHAGS 120 Query: 121 AEVQAFLVALPSVMSGGDAAPWPAATSAATSAPAPAAAEPVVAAAAQLPEPELAEPAPVP 180 AEVQAFLVALPSVMSGGDAAPWPAATSAATSAPAPAAAEPVVAAAAQLPEPELAEPAPVP Sbjct: 121 AEVQAFLVALPSVMSGGDAAPWPAATSAATSAPAPAAAEPVVAAAAQLPEPELAEPAPVP 180 Query: 181 AAPVAGAHLTNASGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAM 240 AAPVAGAHL NASGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAM Sbjct: 181 AAPVAGAHLANASGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAM 240 Query: 241 QALDLLQQKLATTQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYD 300 QALDLLQQKLATTQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYD Sbjct: 241 QALDLLQQKLATTQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYD 300 Query: 301 EALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLR 360 EALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLR Sbjct: 301 EALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLR 360 Query: 361 NAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRRAVVARGL 420 NAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVR AVVARGL Sbjct: 361 NAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGL 420 Query: 421 SNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAG 480 SNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAG Sbjct: 421 SNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAG 480 Query: 481 QGSRFLMQLPLTLSVIRSLLVDISGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGR 540 QGSRFLMQLPLTLSVIRSLLVDI+GEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGR Sbjct: 481 QGSRFLMQLPLTLSVIRSLLVDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGR 540 Query: 541 QVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKI 600 QVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKI Sbjct: 541 QVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKI 600 Query: 601 ADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDD 660 ADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDD Sbjct: 601 ADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDD 660 Query: 661 SLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADRFDLVITDIDMPRMDGIELVSLIRG 720 SLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRAD FDLVITDIDMPRMDGIELVSLIRG Sbjct: 661 SLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADSFDLVITDIDMPRMDGIELVSLIRG 720 Query: 721 AANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDESLLQAVTDLIGEAT 775 AANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDESLLQAVTDLIGEAT Sbjct: 721 AANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDESLLQAVTDLIGEAT 775 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 195 bits (495), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 126/434 (29%), Positives = 228/434 (52%), Gaps = 44/434 (10%) Query: 193 SGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAMQALDLLQQKLAT 252 SG ++V+V +DRL+ L GE +V S+ P+ LA Sbjct: 446 SGNKSVKVEQVKIDRLMNLIGEMVV-SKNALPY------------------------LAQ 480 Query: 253 TQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYDEALACRMRPFAD 312 D+F +R L ++ + Q + ++ +++ + D + RM P + Sbjct: 481 RAEDQFG------VRELARE----IKAQYAVIN-------RIAEEMQDAIMQVRMMPVSV 523 Query: 313 GTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQ 372 R+VRD+ LGK V+L +VG T+ D++I+E L P+ H++RN++DHG+E Q Sbjct: 524 VFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQ 583 Query: 373 RRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRRAVVARGLSNQETAERLSEA 432 RRA+GKP G + + A H ++I + DDG GID ++R +GL ++ ER+S+ Sbjct: 584 RRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQ 643 Query: 433 ELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLT 492 + + + FS + V+++SGRGVG+DVV + +++V G++ + + +G+G+R + LPL+ Sbjct: 644 DAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLS 703 Query: 493 LSVIRSLLVDISGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGRQVGLVSAHQVL- 551 ++V + ++++ G+ + P+ +V T+R+P +L G +GL +Q+L Sbjct: 704 MAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILG 763 Query: 552 -QKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKIADVLAGALME 610 Q + DG V V+V+ + GL +D F ++ +PL LG ++ ALM Sbjct: 764 IAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMG 823 Query: 611 DGTPLLILDVADML 624 DG+ L++L+ +M Sbjct: 824 DGSVLMVLNTREMF 837 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 188 bits (477), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 109/338 (32%), Positives = 185/338 (54%), Gaps = 4/338 (1%) Query: 295 SQRLYDEALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAP 354 ++ L + ++ RM P + RMVRD+ + LGK V G T++D+ ++E++ P Sbjct: 357 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDP 416 Query: 355 IGHLLRNAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRRA 414 + HL+RN++DHGIE RRA+GK E G + L A H G ++IEV DDG G++ E++ Sbjct: 417 LTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAK 476 Query: 415 VVARGLSNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIR 474 +G+ + LS+AE+ + + P FS + VT++SGRGVG+DVV + + G + Sbjct: 477 AKQQGMP---VTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVD 533 Query: 475 ITTQAGQGSRFLMQLPLTLSVIRSLLVDISGEPYAFPLAYVNRTLRLPAEALQTLEGY-Q 533 I + G G+ + LPLTL+++ + + I E Y PL YV +L+ A+ ++ + G Q Sbjct: 534 IRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQ 593 Query: 534 HFSHGGRQVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPL 593 G + L+ +Q+ + VV++ L VD +G++ +VV+ L Sbjct: 594 VLRVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNL 653 Query: 594 DPRLGKIADVLAGALMEDGTPLLILDVADMLRTVEKLT 631 + K+A + ++ DG LI+DVA +LR+ +L Sbjct: 654 ESNYRKVAGISGATILGDGGVALIVDVAALLRSSRQLN 691 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 186 bits (472), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 119/354 (33%), Positives = 192/354 (54%), Gaps = 11/354 (3%) Query: 293 NLSQRLYDEALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLE 352 L +++ D AL RM + R+VRD+ LGK + L + G+ T++D+ ++E+L Sbjct: 379 TLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLA 438 Query: 353 APIGHLLRNAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVR 412 P+ H++RNA+DHGIE +A+R AAGKP + ++L A H +G ++IEV DDG G+D E + Sbjct: 439 DPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAIL 498 Query: 413 RAVVARGLSNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGT 472 VA+GL++ + LS+AE+ F+ P FS VTE+SGRGVG+DVV ++ + G Sbjct: 499 AKAVAQGLASPDA--ELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGE 556 Query: 473 IRITTQAGQGSRFLMQLPLTLSVIRSLLVDISGEPYAFPLAYVNRTLRLPAEALQTLEGY 532 + I T G+G+ ++LPLTL++I V + + PL V + +P + G Sbjct: 557 VSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQ----PGS 612 Query: 533 QHFSHGGRQVGLVSAHQVLQ-KGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQ 591 + + V ++ + R R+ S+ +V G GL VD LGE V++ Sbjct: 613 HIVNVRDEPLPFVPLRELFDLPARERGRN-SLVIVQYGPLR--AGLLVDGLLGECQAVIK 669 Query: 592 PLDPRLGKIADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAA 645 PL GK+ + ++ DG LILD+A ++ ++ G Q + G A Sbjct: 670 PLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQ-QEQGSAQAYAERGVA 722 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 168 bits (425), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 110/349 (31%), Positives = 182/349 (52%), Gaps = 5/349 (1%) Query: 273 NQQLLGEQLSALDAFDRRSVNLSQRLYDEALACRMRPFADGTAGLARMVRDVGNTLGKSV 332 +Q+ L E +S+ R+ ++ L E + RM F + +VR V GK + Sbjct: 379 HQRRLQEGVSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPL 438 Query: 333 RLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQRRAAGKPEQGVIRLEARHSA 392 LD+ QIDR ILE+L P+ HLLRNA HGIES+ QR A GKP G + ++A H Sbjct: 439 ALDLSNCRLQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEG 498 Query: 393 GMLLIEVADDGAGIDIEQVRRAVVARGLSNQETAERLSEAELLEFLLLPSFSLRDTVTEI 452 +I V+DDG G+D+E +R+ + G + A S+A L + + P S V+ + Sbjct: 499 NEAVIRVSDDGRGLDLEAIRQKALELGAAGLAEA---SQARLADLIFQPGLSTSPQVSAL 555 Query: 453 SGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLTLSVIRSLLVDISGEPYAFPL 512 +GRG+G+DVV + + G +R+ +Q GQG +F + LPL+L+V L+ + +A P Sbjct: 556 AGRGIGMDVVRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPS 615 Query: 513 AYVNRTLRLPAEALQTLEGYQHFSHGGRQVGLVSAHQVLQKGQWRSRDG--SVCVVVIGE 570 V+ ++L + + H G ++ L H +L + + +G S V++I Sbjct: 616 MLVDSIVQLRGPQARQALAERSLEHQGSRLALHPWHCLLDEAPEPAGEGSDSAYVLLIKR 675 Query: 571 QEHTYGLAVDAFLGERMLVVQPLDPRLGKIADVLAGALMEDGTPLLILD 619 + + D G R +VV+P+ P+L +A V+ ++ DG +LI++ Sbjct: 676 DDGLMAVLADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIIN 724 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,140,732 Number of extensions: 88417 Number of successful extensions: 557 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 538 Number of HSP's successfully gapped: 39 Length of query: 775 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 682 Effective length of database: 1,179,515 Effective search space: 804429230 Effective search space used: 804429230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)