BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0204.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 677 0.0 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 154 7e-39 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 142 4e-35 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 132 4e-32 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 77 2e-15 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 677 bits (1746), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/342 (99%), Positives = 341/342 (99%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 +KIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 121 EKIDNLGRLSPEMVSPPVRGGRAETLAPAQPKRSDSLPPLVAVGASAGGPAALATLLKAI 180 EKIDNLGRLSPEMVSPPVRGGRAETLAPAQP R+DSLPPLVAVGASAGGPAALATLLKAI Sbjct: 121 EKIDNLGRLSPEMVSPPVRGGRAETLAPAQPARADSLPPLVAVGASAGGPAALATLLKAI 180 Query: 181 PQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVK 240 PQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVK Sbjct: 181 PQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVK 240 Query: 241 EGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAH 300 EGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAH Sbjct: 241 EGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAH 300 Query: 301 TMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQAIIV 342 TMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQAIIV Sbjct: 301 TMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQAIIV 342 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 154 bits (390), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 107/349 (30%), Positives = 180/349 (51%), Gaps = 14/349 (4%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 +K+ IV+D+ ++ V+R +I E++ +A D A ++ PDV+ +D+ MP++D Sbjct: 3 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 62 Query: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 G + ++M P P+++V+S + ++ A+ GA+D P + +G ++ A +I Sbjct: 63 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 122 Query: 121 -EKIDNLGRLSPEMVSPPVR----GGRAETLAPAQPKRSDSLPPLVAVGASAGGPAALAT 175 +KI + GG +E L + + S L+ +GAS GG A+ Sbjct: 123 ADKIRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSS-EKLIIIGASTGGTEAIKE 181 Query: 176 LLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNR 235 L +P G ++I QH+ F AQ L + V + + G +A + Sbjct: 182 FLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 Query: 236 HL-VVKEGGRLGYV----EGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAG 290 HL +V+ G Y+ +G P + +RPSVDV FQS G++LTGMG+DGA G Sbjct: 241 HLKLVRSGA--NYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQG 298 Query: 291 LKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQA 339 + +R++GA+ AQD+ SC V+GMP+ A+A+ AA EVL + M R+ A Sbjct: 299 MLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLA 347 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 105/348 (30%), Positives = 174/348 (50%), Gaps = 11/348 (3%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 +K+ I++D+ +M L +V ++G + I + R+G +AV++ PDVV +D+ MP++D Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFD-ICLGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPA-LIGAAGKRDAAAL 119 G A +M P P+++V+S + A +EAM GA+D P+ I + L Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 120 IEKID--NLGRLSPEMVSPPVRGGRAETLAPAQPKRSDSLPP---LVAVGASAGGPAALA 174 + K+ R+ +P + R AP P LV +G S GGP+ L Sbjct: 121 LAKVRVAAKARIRSRAPAPELARAREPLAAPVAEAARRGRPASDGLVLIGVSTGGPSTLE 180 Query: 175 TLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASN 234 +L A+P F +++ QH+ AAF A+ + + AL V Q G V +A Sbjct: 181 EILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGG 240 Query: 235 RH-LVVKEGGRLGYVEG--APNDVYRPSVDVFFQSIVRHWPGK-VAGLLLTGMGRDGAAG 290 LV K G L + +P ++ PSV++ +S ++ + + G++LTGMG DGAA Sbjct: 241 ADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDGAAA 300 Query: 291 LKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQ 338 + G T+A+ + SC V+GMPK + A VLP + +A +L+ Sbjct: 301 FAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLR 348 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 132 bits (332), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 112/364 (30%), Positives = 179/364 (49%), Gaps = 46/364 (12%) Query: 14 EVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVDGAEATRRIMEATP 73 +++++V+A++ +I A D A++ Q PDV+++D+ MP++DG R+IM P Sbjct: 20 QLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATRP 79 Query: 74 CPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALIEKIDNL------- 126 P+++ ++ + EA+ GA+ P L G RD L + ++ L Sbjct: 80 TPVIMCSAHVGPDNALAAEALAAGAVSVIARPQL----GVRDF--LQQAVEELASAIRAA 133 Query: 127 -----GRLSPEMVS----PPVRGGRAETLAPAQ----------------PKRSDSLPP-- 159 G+ +P+ + PV+ G E L A P D L P Sbjct: 134 MQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPAT 193 Query: 160 --LVAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLA 217 +VA+GAS GGP AL +L + Q G +V+VQH+ F A+ L + S +V A Sbjct: 194 ARIVAIGASTGGPQALERVLGKLSQRCPG-IVVVQHMPQRFTRSFAERLHRISGAEVKEA 252 Query: 218 QEGQAPQRGAVLLAASNRHLVVKEGGRLGYVE---GAPNDVYRPSVDVFFQSIVRHWPGK 274 + G VL+A RHLVV+ G YVE G ++PSVDV F+S+ + Sbjct: 253 EHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSN 312 Query: 275 VAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMA 334 G+++TGMG DGA G++ L D G T AQD+ S V+GMPK A+ +V+ + ++ Sbjct: 313 AVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHIS 372 Query: 335 ARLQ 338 A ++ Sbjct: 373 AVIE 376 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 57/183 (31%), Positives = 95/183 (51%), Gaps = 3/183 (1%) Query: 161 VAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEG 220 + +GASAGG AL+ +L A+ +G A A ++V H+ +AQ + V AQ+ Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 221 QAPQRGAVLLAASNRHLVV---KEGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAG 277 + Q G + A + HL+V ++G L + RP++DV F+S + ++ G Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 Query: 278 LLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARL 337 +LLTG DGAAG++A+ +G T+ Q + V MP A+A+ VL + +A L Sbjct: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIADLL 196 Query: 338 QAI 340 + + Sbjct: 197 RTL 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,060,497 Number of extensions: 45392 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 5 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)