BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0200.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 483 e-138 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 228 4e-61 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 73 2e-14 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 67 2e-12 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 483 bits (1243), Expect = e-138, Method: Compositional matrix adjust. Identities = 230/230 (100%), Positives = 230/230 (100%) Query: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG Sbjct: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 Query: 61 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY Sbjct: 61 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 Query: 121 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD 180 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD Sbjct: 121 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD 180 Query: 181 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR 230 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR Sbjct: 181 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR 230 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 228 bits (581), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 113/212 (53%), Positives = 144/212 (67%), Gaps = 7/212 (3%) Query: 2 IDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGP 61 I +YS TPNG KV IMLEE GLPY AH + DQ +PEFL ++PNNKIPAI+DP+GP Sbjct: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGP 81 Query: 62 DGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLYA 121 G+P+ LFESGAIL+YLA KTG+FL +Y+V+QWVMFQMGG+GPM GQ F +A Sbjct: 82 GGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 Query: 122 PEQIE--YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRS-----HKN 174 ++ E +RY NE++RL VMDK+L Q +L G+ Y+IADIA FPW R+ Sbjct: 142 GKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 Query: 175 QGIDLDDFPNVKRWYDAISARPAVQRGVTVLA 206 + DFP+V R +A ARPAV RG+ + A Sbjct: 202 DLVGFQDFPHVARALEAFVARPAVVRGLNIPA 233 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 72.8 bits (177), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 53/190 (27%), Positives = 85/190 (44%), Gaps = 15/190 (7%) Query: 11 NGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGPDGEPISLFE 70 + H+ + GLP +D+ AG P FL ++P ++P + DGE + + + Sbjct: 11 HAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVL-----EDGE-VVIAD 64 Query: 71 SGAILIYLAGKTGR--FLGETTREKYEVLQWVMFQMGGL--GPMLGQAHHFRLYAPEQIE 126 S AIL+YLA K GR +L E + V +W+ G L GP RL Sbjct: 65 SNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLVTVFGAA 119 Query: 127 YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLDDFPNVK 186 + A L +++ L + +LA +H TIAD+A + + +DL +P V+ Sbjct: 120 FNAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 Query: 187 RWYDAISARP 196 + I A P Sbjct: 180 GFLQRIEALP 189 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 65/232 (28%), Positives = 92/232 (39%), Gaps = 49/232 (21%) Query: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTP-EFLKISPNNKIPAIVDPD 59 MI V+ +V +LEE GL Y P E LK+ P K P I D Sbjct: 1 MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITD-- 58 Query: 60 GPDGEPISLFESGAILIYLAGKTGRFLG----ETTREKYEVLQWVMFQMGGLGP------ 109 GE +++ ESG I+ YL + G G T E+ W+ F G P Sbjct: 59 ---GE-LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQL 114 Query: 110 ------------------MLGQAHHFRLYAPEQIEYAVNRYTNEARRLYQVMDKQLGQHA 151 ML + R+ AP+Q R + M+ +LGQ Sbjct: 115 IFAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQ------------ERQLRFMEDELGQQG 162 Query: 152 YLAGEHYTIADI-ACFPWTRSHKNQGIDLDDFPNVKRWYDAISARPAVQRGV 202 + AG ++ ADI FP G+D +PN+ ++ +AI ARPA QR + Sbjct: 163 WFAGAQFSGADIQMSFPVEALRARGGLD-GRYPNLLKYLEAIHARPAYQRAL 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 816,376 Number of extensions: 36208 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 4 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)