BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0199.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 797 0.0 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 115 7e-27 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 114 1e-26 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 91 1e-19 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 87 2e-18 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 797 bits (2058), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/385 (100%), Positives = 385/385 (100%) Query: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQ 60 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQ Sbjct: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQ 60 Query: 61 YPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE 120 YPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE Sbjct: 61 YPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE 120 Query: 121 PVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVM 180 PVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVM Sbjct: 121 PVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVM 180 Query: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV Sbjct: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 Query: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDL 300 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDL Sbjct: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDL 300 Query: 301 FRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPRE 360 FRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPRE Sbjct: 301 FRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPRE 360 Query: 361 SGIVRFCFAKKDETLQLALERLAKL 385 SGIVRFCFAKKDETLQLALERLAKL Sbjct: 361 SGIVRFCFAKKDETLQLALERLAKL 385 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 115 bits (288), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 105/367 (28%), Positives = 173/367 (47%), Gaps = 35/367 (9%) Query: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYGHRYDPVS 90 +++G G PDF PA+V A T+AM EG QY G+P LR AI++ +YG P Sbjct: 36 IHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEIAP-E 94 Query: 91 EITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDC---YVPAIE----LAGGVPVFVQME 143 I VTAGA+ +L + V EV++ +P Y C +V A E L P + + Sbjct: 95 RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPEH-RFQ 153 Query: 144 VGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDE 203 + AQ W TR +++ +P NPTG+ + ++ A+ VR + DE Sbjct: 154 LSAQMVREHWGSA-------TRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVDE 206 Query: 204 VYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQ 263 +Y+ + Y+G P +L+ L + + +SF K +++TGW++G++ P AL + K+ Q Sbjct: 207 IYQGLSYEGAPFSALS----LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQ 262 Query: 264 FNVFTVNTPVQYGLAAYM--KDPAPYLDLPAFYQHKRDLFRTGLANTRFE--LLPSQGTY 319 + ++ Q+ A + A Y + A ++ +RD L + F ++P Y Sbjct: 263 NLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFY 322 Query: 320 FQCVKYGAISALPE--AEFCKWLTSEIGVAAIPVSAF--YNTPRESGIVRFCFAKKDETL 375 Y SAL + + + +E GV +P F + R +R +A E L Sbjct: 323 V----YADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARR---YIRLSYATSMENL 375 Query: 376 QLALERL 382 Q A+ RL Sbjct: 376 QEAVARL 382 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 93/382 (24%), Positives = 165/382 (43%), Gaps = 14/382 (3%) Query: 7 VTKLPKVGTTIFTVMSALASEKGA--VNLGQGFPDFHCDPALVAAVTQAMQE-GLNQYPP 63 + +LP I + A +G +++ G PD P +V + + Q + Y Sbjct: 44 IERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSS 103 Query: 64 MAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVY 123 G+P LR AIA Y +DP SE VT G+ +G+ +L + GD V+V P Y Sbjct: 104 SKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSY 163 Query: 124 DCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVT---PKTRMIMVNTPHNPTGSVM 180 ++ +AG V+M G + D+++ AV PK +M+++ P NPT + Sbjct: 164 PIHIYGAVIAGANIRSVRMSPGVDFF----DELERAVRESYPKPKMMILGFPSNPTAQCV 219 Query: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 + + R IL++ D Y + +DG S+ + P E + + K+Y++ Sbjct: 220 ELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNM 279 Query: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQY-GLAAYMKDPAPYLDLPAFYQHKRD 299 GW++G++ A L A ++ ++ + TPVQ +AA D + ++ A Y+ +R+ Sbjct: 280 AGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRN 339 Query: 300 LFRTGLANTRFEL-LPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTP 358 + GL + + +P Y EF + L + V P F Sbjct: 340 VLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYG 399 Query: 359 RESGIVRFCFAKKDETLQLALE 380 E VRF + + ++ A+ Sbjct: 400 DE--YVRFALIENESRIRQAIR 419 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 55/163 (33%), Positives = 93/163 (57%), Gaps = 8/163 (4%) Query: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV-FVQMEVGAQ 147 + +I + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV +V E Q Sbjct: 95 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE--QQ 152 Query: 148 GYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEH 207 G+ +K +TP T+ I+V P+NPTG++ + + ++ R ++IL+DE+Y+ Sbjct: 153 GWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDK 212 Query: 208 MVYDGQPHESLARHPELAERS-FIN-SSFGKTYHVTGWKVGYV 248 ++YDG H SLA LA+ FI + K Y G++ G++ Sbjct: 213 VLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWM 252 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 84/330 (25%), Positives = 139/330 (42%), Gaps = 11/330 (3%) Query: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYG-HRYDPV 89 ++LG G P H PA + GL YP G LR AIA+ ++K Y R DP Sbjct: 49 ISLGIGEPK-HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPA 107 Query: 90 SEITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGA 146 +EI G+ + + + +V+ PG V+ P Y Y + LAG P FV + Sbjct: 108 TEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSD-PK 166 Query: 147 QGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYE 206 + ++ + +V V + +++ V +P NPTG+V+ D L + +I SDE Y Sbjct: 167 RNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYS 226 Query: 207 HMVYDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEFRKVH 262 + ++ P + L R + SS K +V G + G+VA AA+ +F Sbjct: 227 EIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYR 286 Query: 263 QFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDLFRTGLANTRFELLPSQGTYFQC 322 ++ ++ +Q +D A Y K T L E+ ++ Sbjct: 287 TYHGAAMSPVIQAASVLAWQDETHVQGNIAKYVTKFSQV-TPLLQEVLEVALPDAAFYLW 345 Query: 323 VKYGAISALPEAEFCKWLTSEIGVAAIPVS 352 K + + + EF + L +E V +P S Sbjct: 346 AKVDKLVNISDTEFAQRLYAEYNVTVLPGS 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,249,712 Number of extensions: 54376 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 159 Number of HSP's successfully gapped: 7 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)