BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0192.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer h... 977 0.0 gi|300313659|ref|YP_003777751.1| amidase family protein [Herbasp... 150 2e-37 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 121 1e-28 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 99 6e-22 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 97 3e-21 >gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer hydrolase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 977 bits (2526), Expect = 0.0, Method: Compositional matrix adjust. Identities = 480/482 (99%), Positives = 481/482 (99%) Query: 1 MHTVSSSSLVRPQDGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVAEKLYHLGEQ 60 MHTVSSSSLVRPQDGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVAEKLYHLGEQ Sbjct: 1 MHTVSSSSLVRPQDGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVAEKLYHLGEQ 60 Query: 61 SVAQGLPDGPFRGVPMLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIF 120 SVAQGLPDGPFRGVP+LTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIF Sbjct: 61 SVAQGLPDGPFRGVPLLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIF 120 Query: 121 GTTTSPEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSL 180 GTTTSPEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSL Sbjct: 121 GTTTSPEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSL 180 Query: 181 RVPASACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAP 240 RVPASACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAP Sbjct: 181 RVPASACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAP 240 Query: 241 YAAPHYRQSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQRTAALCQQLGHHVEET 300 YAAPHYRQSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQRTAALCQQLGHHVEET Sbjct: 241 YAAPHYRQSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQRTAALCQQLGHHVEET 300 Query: 301 CLPVDGLEFYDTVFTIIGSQTRNLMNLLVRMAGQPLDEQALEARHRVILRDKGQLSGADY 360 CLPVDGLEFYDTVFTIIGSQTRNLMNLL RMAGQPLDEQALEARHRVILRDKGQLSGADY Sbjct: 301 CLPVDGLEFYDTVFTIIGSQTRNLMNLLARMAGQPLDEQALEARHRVILRDKGQLSGADY 360 Query: 361 AAAVDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGFSPF 420 AAAVDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGFSPF Sbjct: 361 AAAVDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGFSPF 420 Query: 421 TALCNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQAAPWAHRRPPVC 480 TALCNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQAAPWAHRRPPVC Sbjct: 421 TALCNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQAAPWAHRRPPVC 480 Query: 481 AT 482 AT Sbjct: 481 AT 482 >gi|300313659|ref|YP_003777751.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 506 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 143/466 (30%), Positives = 208/466 (44%), Gaps = 23/466 (4%) Query: 13 QDGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVAEKLYH-------LGEQSVAQG 65 + ++L Q IG +LSP E L I R+E +NP +NAV + EQ+V G Sbjct: 8 KSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAAEQAVIDG 67 Query: 66 LPDGPFRGVPMLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTS 125 P G G+P+ KDL G + GS L + N+ D LV RL+ AG + G T Sbjct: 68 KPLGLLHGLPIGIKDL-EETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAGKTNV 126 Query: 126 PEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPAS 185 PE G + + ++GAT NP++ + GGSSG +AA +A +LP+ G+D GGSLR+PA+ Sbjct: 127 PEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLRIPAA 186 Query: 186 ACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAPYAAPH 245 CGV G +PS GL+P GW +S M V D+ L G P Sbjct: 187 KCGVVGFRPSPGLVP-SERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDPLGYAV 245 Query: 246 YRQSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQ-RTAALCQQLGHHVEETCLPV 304 F + Q LRIG + + + AA+ + + C PV Sbjct: 246 SDSEFASLPQVDLSQ-LRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYV-----KLCEPV 299 Query: 305 D-GLEFYDTVFTIIGSQTRNLMNLLVRMAGQPLDEQALEARHRVILRDKGQLSGADYAAA 363 D + F +I ++ + A D +L R +S D A A Sbjct: 300 DVDMGEAHRAFDVIRAEA----FVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWA 355 Query: 364 VDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGFSPFTAL 423 RR +L +RYDLIL+PT P L ++E VA + ++ + T + Sbjct: 356 QSEQTRIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVA--LENYYRWLALTYV 413 Query: 424 CNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQA 469 + PA+S+P + +G+P G F D +LA AA LEQA Sbjct: 414 VTLATNPAISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQA 459 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 121 bits (304), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 139/480 (28%), Positives = 210/480 (43%), Gaps = 30/480 (6%) Query: 14 DGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVA-----EKLY---HLGEQSVAQG 65 D L L+ I E+S E + +E +E++NP +NA+ E+L +Q +AQG Sbjct: 8 DALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRADQLLAQG 67 Query: 66 LPDGPFRGVPMLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTS 125 G G+P KDL + G S GS L DA V R++ AG G T Sbjct: 68 RYLGWMHGMPQAPKDLAS-CKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIFIGKTNV 126 Query: 126 PEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPAS 185 EFG + + LFG TRN + GGSSG A A +A R+LP+A G+D GSLR PA+ Sbjct: 127 SEFGLGSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGSLRNPAA 186 Query: 186 ACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAPYAAPH 245 V+G +P++G +P G +ST M +V D A + AG D P ++ Sbjct: 187 FNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQCPLSSD- 245 Query: 246 YRQSFLAAATGAAPQ------GLRIGLVTHLPPW-PTHPDCSEAVQRTAALCQQLGHHVE 298 TG A Q G RIG + + + P + + + +G VE Sbjct: 246 ------TDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFADIGCTVE 299 Query: 299 ETCLPVDGLE-FYDTVFTIIGSQTRNLMNLLVRMAGQPLDEQALEARHRVILRDKGQLSG 357 + C P +E ++T ++ Q NL V A P L+ + + L+ Sbjct: 300 Q-CRPDYPMEKLWET--WLVHRQWLVGGNLSVYFA-DPARRGLLKPEAQWEVESGQYLTA 355 Query: 358 ADYAAAVDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGF 417 AD A + R + +L +++D +L P+ P + T + T+H + Sbjct: 356 ADVFRASMARSDWYRAIHKLFEQFDYLLLPSAQMFPFDVA--TKWPRNVAGREMDTYHRW 413 Query: 418 SPFTALCNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQAAPWAHRRP 477 +G PAMSVP+ + GLP+G DD +L +A LE PW+ + P Sbjct: 414 MEVVIGPTLAGLPAMSVPVGFGANGLPMGMQIIGPSRDDRGVLQLAYALEGLRPWSAQAP 473 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 99.4 bits (246), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 137/471 (29%), Positives = 202/471 (42%), Gaps = 71/471 (15%) Query: 37 IERLEQVNPALNAVA-------EKLYHLGEQSVAQGLPDGPFRGVPMLTKDLFTPLAGAR 89 I +Q P+LNA+ E+ L + G P GP G+P+L KD + + Sbjct: 79 IRAYDQQGPSLNAIVTLNPKALEEADQLDRERRQSG-PRGPLHGIPILVKDNYDTV-DMP 136 Query: 90 MSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTSPEFGTSYTTESRLFGATRNPWSLD 149 + G+L L DA V RL++AG I G TT E TT S L G TRNP+ Sbjct: 137 TTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHELAAGVTTVSSLTGFTRNPYDPR 196 Query: 150 HSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPASACGVFGLKPSRGL------MPMGP 203 + GGSSG A VAA G+D GS+R+PA+ +FGL+ +RGL MP+ Sbjct: 197 RAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLFGLRTTRGLASRSGVMPLS- 255 Query: 204 LAGEGWAGMSTSHV---MSLSVRDSAAVLDQLAGA---DLGAPYAAPHYRQSFLAAATGA 257 ST V ++ SV D A +LD G+ D A H +S+ Sbjct: 256 ---------STQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHIPKSYRDGLRAD 306 Query: 258 APQGLRIGLVTHL-PPWPTHPDCSEAVQRTAALCQQLGHHVEETCLP-VDGL-------- 307 + QG RIG++ L P + S A+ + + G V + +P +DGL Sbjct: 307 SLQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGAIVTDVTIPELDGLLSGSSIIP 366 Query: 308 -EF-YDT---VFTIIGSQTRNLMNLLVRMAGQPLDEQALEARHRVIL---RDKGQLSGAD 359 EF YD + + G+ +L +L R L E L R+ V L +DK +L Sbjct: 367 YEFKYDLGAYLQSHPGAPVGSLGEILARGMEHELLEAGLRLRNSVDLQNPKDKEELEKVM 426 Query: 360 YAAAVDYLHAFGRRMAELMQR--YDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGF 417 + A M ++MQ+ D ++ PT+ Q A LI G Sbjct: 427 LKRS-----ALKSLMTDVMQKNHLDTLVYPTI---------------QRKAALIGEPQGG 466 Query: 418 SPFTALCNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQ 468 + L +G PA+++P+ + + GLP+ A + LL +A EQ Sbjct: 467 AMNCQLSATTGLPALALPVGFTEDGLPVSLELLAPEFAEPALLGLAYGWEQ 517 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 97.1 bits (240), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 127/440 (28%), Positives = 184/440 (41%), Gaps = 47/440 (10%) Query: 69 GPFRGVPMLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTSPEF 128 G GVP+ KD+F G R + GS +L + V P DA +V + AG G EF Sbjct: 64 GQLTGVPIAHKDIFVT-RGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCDEF 122 Query: 129 GTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPASACG 188 E+ FG T NPW GGSSG +AA VAAR+ P A D GGS+R PAS CG Sbjct: 123 AMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCG 182 Query: 189 VFGLKPSRG-LMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAPYAAPHYR 247 V G+KP+ G + G +A + + ++ + D +L+ + G D + + Sbjct: 183 VTGIKPTYGSVSRYGMIAFA--SSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 Query: 248 QSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQRTAALCQQLGHHVEETCLPVDGL 307 + F + + QGLRIG+ D AV+ + ++LG + E LP L Sbjct: 241 EDFTRSLNDSL-QGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPKTEL 299 Query: 308 EFYDTVFTII--GSQTRNLMNLL-VRMAGQP-----LDEQALEAR--------HRVILRD 351 V+ +I + NL VR + LD+ ++R R IL Sbjct: 300 SI--PVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVG 357 Query: 352 KGQLSGADYAAAVDYLHA----------FGRRMAELMQRYDLILTPTMAQPPARIGALTV 401 LS Y A YL A F +A ++ D+I+ P P L Sbjct: 358 SYVLSHGYYDAY--YLQAQKIRRLIAQDFQNALAGANRQCDVIMGPV---SPTVAWDLGD 412 Query: 402 REEQSVAELIHTFHGFSPFTALCNASGQPAMSVPLHW----NDQGLPIGSHFCAGFGDDT 457 + VA + FT + +G P MS+P + + P+G + D+ Sbjct: 413 KTNDPVANYLADI-----FTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEA 467 Query: 458 LLLAVAAQLEQAAPWAHRRP 477 LL VA Q +QA W R P Sbjct: 468 RLLNVAHQFQQATDWHLREP 487 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,073 Number of extensions: 70324 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 256 Number of HSP's successfully gapped: 12 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)