BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0184.p (607 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 1206 0.0 gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 327 1e-90 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 73 7e-14 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 1206 bits (3121), Expect = 0.0, Method: Compositional matrix adjust. Identities = 584/588 (99%), Positives = 585/588 (99%) Query: 20 MSDPKFWTALQSQFQSQFAQFKPPAQAFPGFAAMGAPPAGSQVDPTAFLKQLADNGATID 79 MSDPKFWTALQSQFQSQFA FKPPAQAFPGFAAMGAPPAGSQVDPTAFLKQLADNGATID Sbjct: 1 MSDPKFWTALQSQFQSQFAPFKPPAQAFPGFAAMGAPPAGSQVDPTAFLKQLADNGATID 60 Query: 80 PAELVRLQQDYTQQFGALWQSMLAAKTPAITDRRLSAPAWQEHPYFAFQAAAYQLNSRFL 139 PAELVRLQQDYTQQFGALWQSMLAAKTPAITDRRLSAPAWQEHPYFAFQAAAYQLNSRFL Sbjct: 61 PAELVRLQQDYTQQFGALWQSMLAAKTPAITDRRLSAPAWQEHPYFAFQAAAYQLNSRFL 120 Query: 140 LALADAVQGSEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGESLTKGIAQLL 199 LALADAVQGSEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGESLTKGIAQLL Sbjct: 121 LALADAVQGSEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGESLTKGIAQLL 180 Query: 200 ADLQKGHVSQTDETAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCI 259 ADLQKGHVSQTDETAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCI Sbjct: 181 ADLQKGHVSQTDETAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCI 240 Query: 260 NKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDAVVRSIHIVQEI 319 NKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDAVVRSIHIVQEI Sbjct: 241 NKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDAVVRSIHIVQEI 300 Query: 320 SKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQL 379 SKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQL Sbjct: 301 SKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQL 360 Query: 380 ALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNL 439 ALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNL Sbjct: 361 ALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNL 420 Query: 440 PGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDHIVPWKSAYASL 499 PGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDHIVPWKSAYASL Sbjct: 421 PGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDHIVPWKSAYASL 480 Query: 500 GLINTAKRSNNRFVLGASGHIAGVINPASTNKRNYWVNDKAKAGSDADAWLAGATEHPGS 559 GLINTAKRSNNRFVLGASGHIAGVINPAS NKRNYW+NDKAKAGS ADAWLAGATEHPGS Sbjct: 481 GLINTAKRSNNRFVLGASGHIAGVINPASKNKRNYWINDKAKAGSGADAWLAGATEHPGS 540 Query: 560 WWGQWAAFLAKHGGRKVAAPKAAGSTRYKHIEPAPGRYVKVKADQLAA 607 WWGQWAAFLAKHGGRKVAAPKAAGSTRYKHIEPAPGRYVKVKADQLAA Sbjct: 541 WWGQWAAFLAKHGGRKVAAPKAAGSTRYKHIEPAPGRYVKVKADQLAA 588 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 327 bits (839), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 184/495 (37%), Positives = 272/495 (54%), Gaps = 12/495 (2%) Query: 109 ITDRRLSAPAWQEHPYFAFQAAAYQLNSRFLLALADAVQGSEKVRHKL-RFAVQQMIDAM 167 + DRR S P WQ P F ++ L ++ A V G EK L FA +Q +D Sbjct: 100 VADRRFSNPDWQRWP-FNLWHQSFLLAEQWWAAATRDVWGVEKHHADLVAFAARQCLDVF 158 Query: 168 SPANFLATNPVAQQKILETRGESLTKGIAQLLADLQKGHVSQTDETA--FEVGRDVGASE 225 SP+N L TNP + +E RG +L G+ + D+ + + TA F GRDV S Sbjct: 159 SPSNQLLTNPEVLRITMEQRGANLLMGMQNCMNDMIGLLLEKPASTARTFMPGRDVAVSP 218 Query: 226 GSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCINKFYILDLQPHNSLVRYTVEQGHTVF 285 G V+ N + +LI+Y P T+TV PLL++P I K+YILDL P NSL+RY VE+GHTVF Sbjct: 219 GKVVLRNRIMELIRYAPATETVCPEPLLLIPAWIMKYYILDLSPANSLIRYLVEKGHTVF 278 Query: 286 LISWRNADAAVETATWDQYVDDAVVRSIHIVQEISKQPQINALGFCVGGTILSCALALLA 345 +SW+N A + D Y+D ++ V +++ +G+C+GGT+LS A A +A Sbjct: 279 CVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLGGTLLSIAAAAMA 338 Query: 346 ARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQLALREQMIGQRGLMAGRDFASAFSSLR 405 G+ +ASLTLL DF++ G + ++IDE Q++L E + Q G ++ A+AF LR Sbjct: 339 RDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLSQSQMAAAFQMLR 398 Query: 406 PNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTV 465 DLLW+ V YL G DL+ WNAD+T +P M YLR ++L++ L E GR V Sbjct: 399 SYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFLDDDLSE-GRYEV 457 Query: 466 GGEKIDLRKINAPAFIYASRDDHIVPWKSAYASLGLINTAKRSNNRFVLGASGHIAGVIN 525 G+ + L +N P F+ + DH+ PW+S Y +++ + FVL + GH AG+++ Sbjct: 458 DGKPVLLADVNLPVFLVGTVRDHVAPWRSVYK----LHSLCPAEITFVLASGGHNAGIVS 513 Query: 526 PASTNKRNYWVNDKAKAGS--DADAWLAGATEHPGSWWGQWAAFLAKHGGRKVAAPKAAG 583 P + + ++ +A S AD W+AG + GSWW WA +LA+ G V A G Sbjct: 514 PPGHPRSQFQISTRAAGASYRSADEWMAGTSVTTGSWWPAWADWLAQRSGTPVPA-AGMG 572 Query: 584 STRYKHIEPAPGRYV 598 + + APG Y+ Sbjct: 573 YDKAHSLADAPGNYI 587 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 72.8 bits (177), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 74/285 (25%), Positives = 125/285 (43%), Gaps = 30/285 (10%) Query: 250 VPLLIVPPCINKFYIL-DLQPHNSLVRYTVEQG-HTVFLISWRNADAAVETATWDQYVDD 307 VP LIV P ++ D SLV + G V LI W +A A ++ D Y+ D Sbjct: 86 VPTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADMKDLEIDNYLAD 145 Query: 308 AVVRSIHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDF-SD 366 V ++ E+ ++ +G C GG I A++AAR + VA L L + +D + Sbjct: 146 LCV----VIDELGG--RVRLVGLCQGGWI----SAMIAARFPEKVARLVLAGSPIDAGAG 195 Query: 367 TGAIDVYIDEMQLALREQMI-GQRGLMAGRDFASAFSSLRPN--------DLLWNYVESN 417 G + + + L E ++ GLM G + ++ P+ +LL + + Sbjct: 196 EGPLKQMVQRLPLRFYEDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDPA 255 Query: 418 YLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINA 477 YL + A W + +LPG + + H++ +N L G+ G+++DLR+I Sbjct: 256 YLAKRETFA----AWYETTVDLPGRWYLQAIGHIFKDNLLAR-GKYVALGQQLDLRQITC 310 Query: 478 PAFIYASRDDHIVPWKSAYASLGLINT-AKRSNNRFVLGASGHIA 521 P ++ A D I + + L+ T A + + V G GHI Sbjct: 311 PLYLLAGERDDITTPEQVLNAAALVGTPAAQITQKIVPG--GHIG 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,837,804 Number of extensions: 73254 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 3 Length of query: 607 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 516 Effective length of database: 1,188,985 Effective search space: 613516260 Effective search space used: 613516260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)