BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0183.p (246 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 504 e-144 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 189 1e-49 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 166 1e-42 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 141 4e-35 gi|300310680|ref|YP_003774772.1| 3-oxoacyl-[acyl-carrier-protein... 138 5e-34 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 504 bits (1298), Expect = e-144, Method: Compositional matrix adjust. Identities = 246/246 (100%), Positives = 246/246 (100%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN Sbjct: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 Query: 241 GGLYMG 246 GGLYMG Sbjct: 241 GGLYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 189 bits (481), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 102/249 (40%), Positives = 153/249 (61%), Gaps = 6/249 (2%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 + ++A VTG GIG AI T L + G V+ G ++ T A G Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVI---GTATSESGAAAITEYLAALGPEAGRG 62 Query: 61 ---NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLF 117 NV++ E++ A E+V+ + G + +LVNNAGIT+D +M ++WD+VI TNL ++ Sbjct: 63 AVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVG 122 Query: 118 NVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVT 177 +++ V+ GM + GRIINI+SV G+ G GQ NY+ AKAG+ G + ALA+E+ ++ +T Sbjct: 123 RLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNIT 182 Query: 178 VNTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDF 237 VN V+PG+I TDM RA+ + I+ IP+ RLG EEIA VA++AS + Y TG+ Sbjct: 183 VNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTL 242 Query: 238 SLNGGLYMG 246 ++NGG+YMG Sbjct: 243 NVNGGMYMG 251 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 166 bits (420), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 94/238 (39%), Positives = 134/238 (56%), Gaps = 4/238 (1%) Query: 7 YVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVSNWE 66 +VTG GIG AI RL ++GY +V C D+ A + G + + +V+N E Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 67 STKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEG 126 + G +V NAGITRD F M +DWD+V+ TNL+ +NV ++ Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 Query: 127 MCER-GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 M R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 Query: 186 IGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGL 243 I TDM +++I+ IP +R G+PEE+A+ VA++ S + Y T S+NGGL Sbjct: 190 IDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 141 bits (356), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 83/244 (34%), Positives = 130/244 (53%), Gaps = 2/244 (0%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVS 63 R+A VTGG GIG +I +L G+TV+ N K + G + ++ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLV-ADINLDAASKTADALVAAGHRAAPLQMDLG 67 Query: 64 NWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQV 123 + ES AAF + + G DVLVNNAG+ R F ++W ++ N+ + + Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHA 127 Query: 124 IEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSP 183 M ++ +GRI+NISS++G + G+T Y T+KA + G T +A E+A G+TVN+V+P Sbjct: 128 ARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 Query: 184 GYIGTDMVRAIRPE-VLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGG 242 G + T M +A+ E E +P++R G +EIAS V ++A+ E Y TG ++GG Sbjct: 188 GPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 243 LYMG 246 G Sbjct: 248 FVAG 251 >gi|300310680|ref|YP_003774772.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 138 bits (347), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 89/247 (36%), Positives = 129/247 (52%), Gaps = 3/247 (1%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 M K+IA VTG G+G +I ++ GY V A + + A++ G Sbjct: 1 MDKKIALVTGAARGLGESIARKMHAAGYKV-ALADIDLEQVSAVAASLDASGATAMPLAL 59 Query: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 +V AA + + G VLVNNAG +R +++ ++WD V N+ F Sbjct: 60 DVRRKPDFIAACDAIELRWGGAAVLVNNAGRSRVAALMEITPEEWDEVTAINMKGTFLAC 119 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQ-TNYSTAKAGIHGFTMALAQEVATKGVTVN 179 + + +RG+GRIINI+S+ GQ G +Y+ AK + T A+E+A GVTVN Sbjct: 120 QVFGKRFADRGYGRIINIASLAGQNGGTATGAHYAAAKGAVLTLTKVFARELAGAGVTVN 179 Query: 180 TVSPGYIGTDMVRAIRP-EVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFS 238 +SPG + +VR I P + L ++ IPVK LGEP+ IA V +AS E G ATG+ Sbjct: 180 AISPGPLDLPIVREIVPADKLAATIATIPVKALGEPDFIADAVLLLASPEAGSATGACLD 239 Query: 239 LNGGLYM 245 +NGGLYM Sbjct: 240 INGGLYM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 806,982 Number of extensions: 32325 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 47 Length of query: 246 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 163 Effective length of database: 1,226,865 Effective search space: 199978995 Effective search space used: 199978995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)