BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0178.p (392 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 802 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 126 3e-30 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 107 2e-24 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 802 bits (2071), Expect = 0.0, Method: Compositional matrix adjust. Identities = 389/392 (99%), Positives = 392/392 (100%) Query: 1 VSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 +SDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY Sbjct: 1 MSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 Query: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD Sbjct: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 Query: 121 LGQWLERDFQITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAK 180 LGQWLERDFQITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAK Sbjct: 121 LGQWLERDFQITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAK 180 Query: 181 GVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAH 240 GVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAH Sbjct: 181 GVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAH 240 Query: 241 MRLGFGVGMDDVYMVLRSLSSMRLRFDAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGH 300 MRLGFGVGMDDVYMVLRSLSSMRLRF+AHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGH Sbjct: 241 MRLGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGH 300 Query: 301 AHWKRDFSGAGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMKGMRR 360 AHWKRDFSGAGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMKGMRR Sbjct: 301 AHWKRDFSGAGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMKGMRR 360 Query: 361 AWVREGQLVRFNIGLESPEDLIADIEQALARL 392 AW+REGQLVRFNIGLESPEDLIADIEQALARL Sbjct: 361 AWMREGQLVRFNIGLESPEDLIADIEQALARL 392 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 126 bits (317), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 118/420 (28%), Positives = 179/420 (42%), Gaps = 45/420 (10%) Query: 12 QTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTSFVL 71 +T VH Y A V I+ F D + G Y PT VL Sbjct: 10 ETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTQDVL 69 Query: 72 EARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDFQI 131 E RLA +EGG SG AA+ ++GD+++ +YG + +L + + Sbjct: 70 EKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQYGV 129 Query: 132 TARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDNTWS 191 RF DP LI T+ ++ E+ G+ + DI A+ AH GV + +DNT + Sbjct: 130 QTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGVPLIVDNTVA 189 Query: 192 AGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------------------- 225 + FEHG DIV+ ++TKY G L GA+ Sbjct: 190 TPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKRLNEPDVSYH 249 Query: 226 -IVRDQALNEKLELAHMR------LGFGVGMDDVYMVLRSLSSMRLRFDAHDAAARKVAA 278 +V +AL E + R +G + + + +L+ + ++ LR D A VA Sbjct: 250 GVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRITANTLAVAQ 309 Query: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSG-AGGLFSVIFDPRYVEAQTDRFVDSLRLF 337 +LK+ P++ V + ED P HA + F G A G+ + F RF D+L+LF Sbjct: 310 YLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLT--FGVANGREGGARFQDALQLF 367 Query: 338 KIGYSWGGAHSLC-----VPYRM---KGMRRAWVREGQLVRFNIGLESPEDLIADIEQAL 389 + G A SL +R + +A V E VR +IG+E +DL+AD+EQAL Sbjct: 368 TRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTE-DTVRLSIGIEHIDDLLADLEQAL 426 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 106/425 (24%), Positives = 177/425 (41%), Gaps = 50/425 (11%) Query: 1 VSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 ++D K T T+++HS + + IH + T + D + ++ GY Y Sbjct: 1 MNDKKTYGFT--TTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRY 58 Query: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 G G PT LE ++ ++E GK +G+AAI + L+ GD V+ ++G + Sbjct: 59 GRQGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNS 118 Query: 121 LGQWLERDFQ-ITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHA 179 L W+ Q D + + I TRLV+ E + +V D+ + Sbjct: 119 L--WMTVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRE 176 Query: 180 KGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQ--------- 230 +G+L +DNT ++ F+ G +V+ ++TK G + L GA+ + Sbjct: 177 RGILYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHI 236 Query: 231 ALNEKLE------LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFDAHDAAARKVAA 278 A N K +A +R G +G + + + ++ LR + A +A Sbjct: 237 AENYKKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQ 296 Query: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSGAGGLFSV-------IFDPRYVEAQTDRFV 331 L+ + AV +P E P HA K F G L S FD ++ Sbjct: 297 MLQADERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFD----------YL 346 Query: 332 DSLRLFKIGYSWGGAHSLCVP------YRMKGMRRAWVREGQ-LVRFNIGLESPEDLIAD 384 + LRL + G +L +P Y M RRA + + L+R ++GLE +DL+AD Sbjct: 347 NRLRLAIPTSNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVAD 406 Query: 385 IEQAL 389 QAL Sbjct: 407 FRQAL 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,325,095 Number of extensions: 57195 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 3 Length of query: 392 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 305 Effective length of database: 1,207,925 Effective search space: 368417125 Effective search space used: 368417125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)