BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0167.p (903 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 1845 0.0 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 706 0.0 gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 100 7e-22 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 1845 bits (4778), Expect = 0.0, Method: Compositional matrix adjust. Identities = 902/903 (99%), Positives = 903/903 (100%) Query: 1 MSRNTLNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVT 60 MSRNTLNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVT Sbjct: 1 MSRNTLNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVT 60 Query: 61 EEHVKQLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEP 120 EEHVKQLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEP Sbjct: 61 EEHVKQLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEP 120 Query: 121 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGI Sbjct: 121 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 Query: 181 VHQVNLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY 240 VHQVNLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY Sbjct: 181 VHQVNLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY 240 Query: 241 FLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATI 300 FLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATI Sbjct: 241 FLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATI 300 Query: 301 ANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQE 360 ANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQE Sbjct: 301 ANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQE 360 Query: 361 VSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADG 420 VSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADG Sbjct: 361 VSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADG 420 Query: 421 VDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY 480 VDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY Sbjct: 421 VDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY 480 Query: 481 LEAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEAR 540 LEAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEAR Sbjct: 481 LEAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEAR 540 Query: 541 IHPNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLK 600 IHPNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLK Sbjct: 541 IHPNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLK 600 Query: 601 FAMNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWPTSTYIAEPPFFENFSEEPKAAA 660 FAMNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWP+STYIAEPPFFENFSEEPKAAA Sbjct: 601 FAMNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWPSSTYIAEPPFFENFSEEPKAAA 660 Query: 661 AGITGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIM 720 AGITGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIM Sbjct: 661 AGITGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIM 720 Query: 721 MRGTFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGE 780 MRGTFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGE Sbjct: 721 MRGTFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGE 780 Query: 781 EYGTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQSLGITGD 840 EYGTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQSLGITGD Sbjct: 781 EYGTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQSLGITGD 840 Query: 841 ETFDLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILPFVLRQL 900 ETFDLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILPFVLRQL Sbjct: 841 ETFDLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILPFVLRQL 900 Query: 901 LAA 903 LAA Sbjct: 901 LAA 903 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 706 bits (1821), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/900 (41%), Positives = 548/900 (60%), Gaps = 41/900 (4%) Query: 6 LNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVTEEHVK 65 +NT ++ G++ F + A++ LP + R++ E+++R CD +T+ ++ Sbjct: 1 MNTQYRKQLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDS-LR 59 Query: 66 QLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEPLVPVD 125 QL + R + P+ ARVV D G L DLA +R+ + +G +P + P+VPV Sbjct: 60 QLI----ERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQ 115 Query: 126 LVVDHSVQIDHF-REKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGIVHQV 184 L+VDHS+ ++ + +A N +E RN +R+ F+ W AF+ V+PPG GI+HQ+ Sbjct: 116 LIVDHSLAVECGGDDPQAFVKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQI 175 Query: 185 NLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTP 244 NLE ++ +H K GV +PDTLVGTDSHT ++ +GV+ GVGG+EAE MLG+ + P Sbjct: 176 NLEKMSPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLP 235 Query: 245 DVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANMA 304 D++GV LTG + G+TATD+VL++TE LR+ KVVG ++EF+G+G SL+L DRATI+NMA Sbjct: 236 DIIGVELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMA 295 Query: 305 PEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQEVSLD 364 PEYGAT F +D TI+Y K TGR ++ E Y K L+ A +++Y + + D Sbjct: 296 PEYGATAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLA-KVEYERVLRFD 354 Query: 365 LGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADGVDLH 424 L TV +LAGP P R+ + + A+ + +N G + Sbjct: 355 LSTVVRTLAGPSNPHKRLPVSEL-------------------AAQGIAGKVENEPG-KMP 394 Query: 425 NGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAA 484 +G V+IAAITSCTNTSNP ++AAGLLA+ A GL P +K+SLAPGS+ V YLE A Sbjct: 395 DGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLARKPWVKSSLAPGSKAVELYLEEA 454 Query: 485 GLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIHPN 544 GL LEKLGFG+ A+ CTTC G +G L P + + I+ D+ + AVLSGNRNF+ RIHP Sbjct: 455 GLTADLEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPY 514 Query: 545 IRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLKFAMN 604 + FLASPPLVVAYAIAG + D+ + +G G++I L DIWP+ +EI+ ++ A+ Sbjct: 515 AKQAFLASPPLVVAYAIAGTIRFDIEQDVLGVTAEGREIRLKDIWPSDEEIDAVVTAAVK 574 Query: 605 AKTFKENYADVKGAPGKLWEAIKGVAKGEVYNW-PTSTYIAEPPFFENFSEEPKAAAAGI 663 + F+ Y + A + + +Y+W P STYI PP++E A + Sbjct: 575 PQQFRNVYTPMFAA-----RVDRSQSVSPLYDWRPQSTYIRRPPYWEG----ALAGERTL 625 Query: 664 TGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIMMRG 723 +G R L V GD+ITTDH+SP+ +I S AG++L G+ + DFNSY + RG+H R Sbjct: 626 SGMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRA 685 Query: 724 TFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGEEYG 783 TFAN ++ N M+ K DGS +G + +P G+ M +++ Y+ P ++ G +YG Sbjct: 686 TFANPKLFNEMV-KKADGSVQQGSLARLEPEGKVMRMWETIETYMERKQPLIIIAGADYG 744 Query: 784 TGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQSLGITGDETF 843 GSSRDWAAKG +L GV+A++A FERIHR+NL+GMGVLPL+F ++ + I G ET+ Sbjct: 745 QGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFKAGENRNTYAIDGTETY 804 Query: 844 DLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILPFVLRQLLAA 903 D+ G+ +P+ + TLVIHRKNGE EV + R+DT EV Y+ GG+L + L A Sbjct: 805 DVI---GQRRPRADLTLVIHRKNGERVEVPVTCRLDTAEEVSIYEAGGVLQRFAQDFLEA 861 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 100 bits (248), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 105/372 (28%), Positives = 152/372 (40%), Gaps = 35/372 (9%) Query: 171 FGVVPPGFGIVHQVNLEYLARGVHNKTGVYYPD-TLVGTDSHTTMINGIGVVGWGVGGIE 229 FG+ GIVH + E G P T+V DSHT+ + G+G E Sbjct: 98 FGMNDKRQGIVHVIGPE---------QGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSE 148 Query: 230 AEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGT 289 E + Q + + V + GAL GVTA D+VL + + G +EF G Sbjct: 149 VEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTI 208 Query: 290 ESLSLTDRATIANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGV 349 SLS+ R T+ NMA E GA G DD T+EY KG + + A + L+ Sbjct: 209 RSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYLKGRPFSPSGPHWERAVSYWRSLHSD 268 Query: 350 PKAGQIDYTQEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAE 409 P A + V+L+ + P + P+ + + + + D PT +G +KA Sbjct: 269 PGA---KFDMVVTLNAAEIKPQVTWGTSPEMVVAV-DARVPDPDKEKDPTKRDGM-EKAL 323 Query: 410 DLGASYKNADGVDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTS 469 A N D+ V I SCTN+ + AA ++ K + +K+A Sbjct: 324 AYMALKPNTPIEDIRIDKVFIG---SCTNSRIEDLREAAKVVRGKFRASNVKLA-----M 375 Query: 470 LAPGSRVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAA 528 + PGS +V + E GL GF GC+ C+ NA L P A Sbjct: 376 VVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGERCAS-------- 427 Query: 529 AVLSGNRNFEAR 540 + NRNFE R Sbjct: 428 ---TSNRNFEGR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,681,524 Number of extensions: 112096 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 3 Length of query: 903 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 809 Effective length of database: 1,174,780 Effective search space: 950397020 Effective search space used: 950397020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 135 (56.6 bits)