BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0145.p (305 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 510 e-146 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 162 2e-41 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 69 5e-13 gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 61 1e-10 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 510 bits (1314), Expect = e-146, Method: Compositional matrix adjust. Identities = 301/305 (98%), Positives = 301/305 (98%), Gaps = 3/305 (0%) Query: 1 VERTSSTGKVVDAATAPAAPAPSGRGYYTVKKGDTLYRIALEAGQNWRDIVAWNNLTNPN 60 VERTSSTGKVVDAATAPAAPAPSGRGYYTVKKGDTLYRIALEAGQNWRDIVAWNNLTNPN Sbjct: 31 VERTSSTGKVVDAATAPAAPAPSGRGYYTVKKGDTLYRIALEAGQNWRDIVAWNNLTNPN 90 Query: 61 DIKVDQTLRVAPPDRAPGTAQTAAIAASPAAGLEVKPLGAPGTSAPAAPVPVGSNKTGPR 120 DIKVDQTLRVAPPDRAPGTAQTAAIAASPAAGLEVKPLGAPGTSAPAAPVPVGSNKTGPR Sbjct: 91 DIKVDQTLRVAPPDRAPGTAQTAAIAASPAAGLEVKPLGAPGTSAPAAPVPVGSNKTGPR 150 Query: 121 GDKRPYSESALAELEKPDAPSAPAASGTSGTSVASAAPSAPAKAEQPHAADKPAAAASQA 180 GDKRPYSESALAELEKPDAPSAPAASGTS VASAAPSAPAKAEQPHAADKPAAAASQA Sbjct: 151 GDKRPYSESALAELEKPDAPSAPAASGTS---VASAAPSAPAKAEQPHAADKPAAAASQA 207 Query: 181 ATDDEGVSWMWPAEGKVVGTFEGGKKGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLV 240 A DDEGVSWMWPAEGKVVGTFEGGKKGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLV Sbjct: 208 AADDEGVSWMWPAEGKVVGTFEGGKKGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLV 267 Query: 241 IIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKSDSDTVKLHFEIRQQGKPVDPSK 300 IIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKSDSDTVKLHFEIRQQGKPVDPSK Sbjct: 268 IIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKSDSDTVKLHFEIRQQGKPVDPSK 327 Query: 301 YLPPR 305 YLPPR Sbjct: 328 YLPPR 332 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 162 bits (411), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 85/151 (56%), Positives = 104/151 (68%), Gaps = 9/151 (5%) Query: 157 APSAPAK--AEQPHAADKPAAAASQAATDDEGVSWMWPAEGKVVGTFEGGKKGVDIAGKA 214 APS P A +P D PA A ++ V W WP GK + + KKG+DI G Sbjct: 111 APSRPTAKPAAKPQDDDAPAGAEAK-------VDWSWPTAGKSAPSADSYKKGIDIIGSE 163 Query: 215 GQAVLAAGAGKVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAE 274 GQ+V+AA AGKV YAG GIRGYGN+VIIKHT LLS YAHN+TILVKEGQ+VT+GQ+IA Sbjct: 164 GQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVKEGQTVTRGQQIAT 223 Query: 275 MGKSDSDTVKLHFEIRQQGKPVDPSKYLPPR 305 MG SD++ VKL+FEIR+ GKP++ LP R Sbjct: 224 MGNSDTNKVKLYFEIRRNGKPINVMAVLPAR 254 Score = 56.2 bits (134), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 24/48 (50%), Positives = 33/48 (68%) Query: 26 GYYTVKKGDTLYRIALEAGQNWRDIVAWNNLTNPNDIKVDQTLRVAPP 73 GYYTVK+GDTLY I + Q+ ++ WN L NP+D++V Q + V PP Sbjct: 32 GYYTVKRGDTLYSIGRDFDQSPANLRTWNRLRNPDDLEVGQRILVRPP 79 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 39/121 (32%), Positives = 66/121 (54%), Gaps = 8/121 (6%) Query: 192 PAEGKVVGTFEGGK------KGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHT 245 P +G ++ F + +G+ I G V A AG+V++A +RG+GNL+I+ H Sbjct: 351 PVKGDLMAKFGSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFA-DWLRGFGNLIIVDHG 409 Query: 246 SNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKS-DSDTVKLHFEIRQQGKPVDPSKYLPP 304 + ++ Y +NQ++L + G V G IA G S ++ L+FE+R QG+ DP ++ Sbjct: 410 NQYMTIYGNNQSVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTS 469 Query: 305 R 305 R Sbjct: 470 R 470 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 27/68 (39%), Positives = 43/68 (63%), Gaps = 1/68 (1%) Query: 236 YGNLVIIKHTSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKSDSDT-VKLHFEIRQQGK 294 +GN++ I H +N+++ YAH + VK G V +GQ +A++G + T LHFE+R +G Sbjct: 238 FGNMLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGI 297 Query: 295 PVDPSKYL 302 DP K+L Sbjct: 298 AQDPRKFL 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.308 0.126 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,732 Number of extensions: 30898 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 5 Length of query: 305 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 220 Effective length of database: 1,217,395 Effective search space: 267826900 Effective search space used: 267826900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 130 (54.7 bits)