BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0131.p
         (374 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312251|ref|YP_003776343.1| GTP-binding protein [Herbaspiri...   748   0.0  
gi|300313619|ref|YP_003777711.1| tRNA modification (thiophene/fu...    56   4e-09

>gi|300312251|ref|YP_003776343.1| GTP-binding protein
           [Herbaspirillum seropedicae SmR1]
          Length = 374

 Score =  748 bits (1931), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 374/374 (100%), Positives = 374/374 (100%)

Query: 1   MRAALVGLDFGKNDFAASLDELYLLAKSAGAEPVITITGRRASPDAALFIGTGKAQEVAD 60
           MRAALVGLDFGKNDFAASLDELYLLAKSAGAEPVITITGRRASPDAALFIGTGKAQEVAD
Sbjct: 1   MRAALVGLDFGKNDFAASLDELYLLAKSAGAEPVITITGRRASPDAALFIGTGKAQEVAD 60

Query: 61  AVADLQLELVIFNHALSPAQQRNLERLLKVRVLDRTSLILDIFAQRAKSHEGKVQVELAQ 120
           AVADLQLELVIFNHALSPAQQRNLERLLKVRVLDRTSLILDIFAQRAKSHEGKVQVELAQ
Sbjct: 61  AVADLQLELVIFNHALSPAQQRNLERLLKVRVLDRTSLILDIFAQRAKSHEGKVQVELAQ 120

Query: 121 LQHLSTRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLGERVKALRAVLAKLRRQHATQ 180
           LQHLSTRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLGERVKALRAVLAKLRRQHATQ
Sbjct: 121 LQHLSTRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLGERVKALRAVLAKLRRQHATQ 180

Query: 181 RRARGRNETFSVSLVGYTNAGKSTIFNALAKAGVYAANQLFATLDTTSRRVYLGEVGHVV 240
           RRARGRNETFSVSLVGYTNAGKSTIFNALAKAGVYAANQLFATLDTTSRRVYLGEVGHVV
Sbjct: 181 RRARGRNETFSVSLVGYTNAGKSTIFNALAKAGVYAANQLFATLDTTSRRVYLGEVGHVV 240

Query: 241 ISDTVGFIRELPHQLVEAFRATLEETIHADLLLHVVDAASPVRMEQIEEVNLVLKEIGAD 300
           ISDTVGFIRELPHQLVEAFRATLEETIHADLLLHVVDAASPVRMEQIEEVNLVLKEIGAD
Sbjct: 241 ISDTVGFIRELPHQLVEAFRATLEETIHADLLLHVVDAASPVRMEQIEEVNLVLKEIGAD 300

Query: 301 HVPQILVWNKIDAAGLEPTVEYDEYGKIQRVFVSAKSGAGLDLLREAIAASLKAALEARG 360
           HVPQILVWNKIDAAGLEPTVEYDEYGKIQRVFVSAKSGAGLDLLREAIAASLKAALEARG
Sbjct: 301 HVPQILVWNKIDAAGLEPTVEYDEYGKIQRVFVSAKSGAGLDLLREAIAASLKAALEARG 360

Query: 361 RSRSEPVDSVDMHA 374
           RSRSEPVDSVDMHA
Sbjct: 361 RSRSEPVDSVDMHA 374


>gi|300313619|ref|YP_003777711.1| tRNA modification (thiophene/furan
           oxidation) GTPase [Herbaspirillum seropedicae SmR1]
          Length = 479

 Score = 56.2 bits (134), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 52/160 (32%), Positives = 78/160 (48%), Gaps = 17/160 (10%)

Query: 194 LVGYTNAGKSTIFNALAKAGVYAANQLFATL-DTTSRRVYLGEVGHVVISDTVGFIREL- 251
           L G  N GKS++ N LA + V     +  T  D  ++ + + E   + I DT G IRE  
Sbjct: 239 LAGKPNVGKSSLLNVLAGSDVAIVTPIAGTTRDKVTQTIQI-EGMPLNIIDTAG-IREAG 296

Query: 252 -----PHQLVE-AFRATLEETIHADLLLHVVDAASPVRMEQIEEVNLVLKEIGADHVPQI 305
                P ++       T  E   AD++LH++DA    R   +E+  +  +    D VP I
Sbjct: 297 DDGSGPDEVERIGIERTWAEVAKADVILHMLDAD---RGPTLEDEKITSR--FPDGVPII 351

Query: 306 LVWNKIDAAGLEPTVEYDEYGKIQRVFVSAKSGAGLDLLR 345
            +WNKID +G +  V  D+      V++SA  G G+DLLR
Sbjct: 352 RIWNKIDRSGHKAAV--DKMPDATHVYLSASEGQGVDLLR 389


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.134    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,063,011
Number of extensions: 43558
Number of successful extensions: 257
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 256
Number of HSP's successfully gapped: 2
Length of query: 374
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 287
Effective length of database: 1,207,925
Effective search space: 346674475
Effective search space used: 346674475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)