BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0125.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 760 0.0 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 334 7e-93 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 133 2e-32 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 113 3e-26 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 62 9e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 760 bits (1962), Expect = 0.0, Method: Compositional matrix adjust. Identities = 395/395 (100%), Positives = 395/395 (100%) Query: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA Sbjct: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 Query: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV Sbjct: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 Query: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR Sbjct: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 Query: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI Sbjct: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 Query: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGM 300 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGM Sbjct: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGM 300 Query: 301 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA 360 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA Sbjct: 301 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA 360 Query: 361 ALLPLAGIVVALVSWKMDAGRATAQHGSSSDTAAC 395 ALLPLAGIVVALVSWKMDAGRATAQHGSSSDTAAC Sbjct: 361 ALLPLAGIVVALVSWKMDAGRATAQHGSSSDTAAC 395 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 334 bits (857), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 188/374 (50%), Positives = 247/374 (66%) Query: 5 LWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGR 64 L+AL ++AF IGTTEFVIMGLLP+VA +LGV+ +AG+LV+GYALGV GAP+L T + Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 65 LPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEK 124 PRK LV LM +F++GN+ CA+AP+Y LM+ RVV +F H AFFG+GSVVA LV +K Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 125 QASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG 184 +A A+ALMFTGLTVAN+LGVP GT G + GWR+TF AVT IGV+A ++P+ Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 185 EETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLF 244 + + +E R ++ +V L LL + L +F+ TYIA IL D++GF S +LLLF Sbjct: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLF 244 Query: 245 GLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAFA 304 G G+ +G L GGKLAD KLM + G + +A VL LF +T K + + G AFA Sbjct: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAWGAFAFA 304 Query: 305 TVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVAALLP 364 VP Q+RVL+ A GAPNLAS+ N AFNLGNA GAW+GG+ I G L +P + A Sbjct: 305 VVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIGAFFA 364 Query: 365 LAGIVVALVSWKMD 378 L G+ S +D Sbjct: 365 LLGLATVAFSASLD 378 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 133 bits (335), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 116/367 (31%), Positives = 188/367 (51%), Gaps = 25/367 (6%) Query: 8 LTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLPR 67 L + FAIGT F+I LLP +AA+ G+S+ LV + L + + +P+ T + + R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQAS 127 + +L+A M F NL A + +T L++ R++ + A G + S +A ++V ++ Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEET 187 A+A++ G+T + LG+P G +G ++GWRATF AV+ +G +ALA + VPR G Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 188 DFGKEVR--VLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFG 245 G R V+ +PQV L +T+L G +AA+ YIAP L+ + F S +S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 LGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAA------------II 293 +G GG L D+ GS ++ L+L L+ AA ++ Sbjct: 269 AAAAVGVSVGGTLNDRY------GSARVVRVSLLLLMLSFWTLAAATALPPPLALGPVLL 322 Query: 294 GVGLMGMAAFATVPPLQMRVL-----EKATGAPNLASSANIAAFNLGNAAGAWLGGVTID 348 V + G + +A P R++ +A A +L +S F+LG+AAGA + G Sbjct: 323 AVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNTSTLYLGFSLGSAAGAGILGSGAI 382 Query: 349 HGPGLHA 355 G GL A Sbjct: 383 WGIGLFA 389 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 105/343 (30%), Positives = 177/343 (51%) Query: 7 ALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLP 66 +L + F++ T EF+ LL +A++L VS +AG VT A+ V AP + T + Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQA 126 RK +++ L ++ +V N+ AP+ L++ RV+ A G F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEE 186 A++L+ TG++VA V P G ++G +GWR+ F A V+ ++A+A + +PR + Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 187 TDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFGL 246 +L R V + LL +L G FA FTYI P++ ++ + +S +LL +G+ Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLLGYGV 280 Query: 247 GLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAFATV 306 G GN AGG +A++ ++ L+A + + + V A + + L G A A Sbjct: 281 GGFFGNFAGGFIAERSERYAIVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFP 340 Query: 307 PPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDH 349 Q ++ A A +AAF + A+GA GGV +DH Sbjct: 341 VGFQTWIVRAAPDHAEGAGGLLVAAFQVAIASGAIGGGVLVDH 383 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 62.0 bits (149), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 62/261 (23%), Positives = 108/261 (41%), Gaps = 13/261 (4%) Query: 29 VAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLPRKTLLVALMVVFVVGNLACAIA 88 + ++ ++ GL+V+ YA L R RK LL+ FV+G + C +A Sbjct: 48 IMPSMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLA 107 Query: 89 PDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQASAIALMFTGLTVANVLGVPFGT 148 + +L++ R+V G + +AT L + + ++ T + VLG+P G Sbjct: 108 QSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGL 167 Query: 149 WLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQ--------GGEETDFGKEVRVLMRPQ 200 +L + W F A+ +G LA V++ R Q E + + L P+ Sbjct: 168 YLSNRWNWHVPFLAMAALG---LAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPR 224 Query: 201 VMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFGLGLVIGNLAGGKLAD 260 + T L G F + + L G + + I L+ GL + G+ AD Sbjct: 225 YLTAFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAAD 284 Query: 261 K--KLMATLLGSLVLLAAVLV 279 K KL L G+++ + VL+ Sbjct: 285 KLGKLRVFLFGAVLSIIMVLI 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 992,692 Number of extensions: 35869 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 306 Number of HSP's successfully gapped: 21 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)