BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0124.p (293 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312244|ref|YP_003776336.1| LysR family transcription regul... 592 e-171 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 189 2e-49 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 174 1e-44 gi|300311929|ref|YP_003776021.1| LysR family transcription regul... 170 1e-43 gi|300312334|ref|YP_003776426.1| transcription regulator protein... 164 9e-42 >gi|300312244|ref|YP_003776336.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 293 Score = 592 bits (1527), Expect = e-171, Method: Compositional matrix adjust. Identities = 293/293 (100%), Positives = 293/293 (100%) Query: 1 MSLQSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELT 60 MSLQSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELT Sbjct: 1 MSLQSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELT 60 Query: 61 EEGRAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYP 120 EEGRAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYP Sbjct: 61 EEGRAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYP 120 Query: 121 DIELELTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPE 180 DIELELTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPE Sbjct: 121 DIELELTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPE 180 Query: 181 SVEALEQHQLIGFSQPDQLNHWPLRHSAGDRYQTRPAIRASSGETIRHLALQGQGIACLS 240 SVEALEQHQLIGFSQPDQLNHWPLRHSAGDRYQTRPAIRASSGETIRHLALQGQGIACLS Sbjct: 181 SVEALEQHQLIGFSQPDQLNHWPLRHSAGDRYQTRPAIRASSGETIRHLALQGQGIACLS 240 Query: 241 NFMSDADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQEKL 293 NFMSDADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQEKL Sbjct: 241 NFMSDADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQEKL 293 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 189 bits (481), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 106/290 (36%), Positives = 162/290 (55%), Gaps = 2/290 (0%) Query: 6 EELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEEGRA 65 + ++TFVAVVE+G+ AA ++ Q+ VSR +S LE LG LL RTTRR+ LT+EGR Sbjct: 5 QAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTDEGRV 64 Query: 66 FLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDIELE 125 + + + +L ++ + + L + + G+LR+ A F + + P G+F +P I L Sbjct: 65 YFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPLITLN 124 Query: 126 LTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPESVEAL 185 +T +DR IDL+E D+AIRI L DS + AR L+ R+ A+P +LARHG P + L Sbjct: 125 ITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGTPAEL 184 Query: 186 EQHQLIGFSQPDQLNHWPLRHSAGDRYQ--TRPAIRASSGETIRHLALQGQGIACLSNFM 243 + I +S + W RH GD Q R + ++G T R +A+ GQGIA +F+ Sbjct: 185 RDYPFISYSYRAGGDDWSFRHVHGDTCQVRVRSTVYVNNGATCRAMAIAGQGIAIQPDFI 244 Query: 244 SDADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQEKL 293 +A I AG+LV L +G + + +Y T L ++ DFL E+ Sbjct: 245 VNAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLIEQF 294 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 174 bits (440), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 108/289 (37%), Positives = 158/289 (54%), Gaps = 8/289 (2%) Query: 7 ELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEEGRAF 66 E+ F+AV + G+ AAE L + + VSR + LE +LG+ L+ RTTRR+ LTE G + Sbjct: 6 EIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTEAGALY 65 Query: 67 LEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDIELEL 126 LE+ + I+AA+E A D + + KP G LR++A F + + P F +P+I L++ Sbjct: 66 LERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPEISLDI 125 Query: 127 TTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPESVEALE 186 T +DR +D+++ D+AIRI L DS+L R L+S L+V ASPAYL HG P L Sbjct: 126 TLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAHPHELA 185 Query: 187 QHQLIGFSQPDQLNHWPLRHSAGD---RYQTRPAIRASSGETIRHLALQGQGIACLSNFM 243 QHQ I +S + W G R +R + ++G++ AL G GI FM Sbjct: 186 QHQTISYSYNQSKDEWQFNGPEGPVSVRVSSR--LHVNNGDSCVQAALGGIGITRQPTFM 243 Query: 244 SDADIAAGRLVRLLPQADSGYRQ-KISAVYYYSTQLSRRISCFLDFLQE 291 D + +G+LV +L D G + I AVY L ++ LDFL E Sbjct: 244 IDQYLRSGQLVSIL--GDYGAPELGIYAVYPSRAHLPAKMRAMLDFLTE 290 >gi|300311929|ref|YP_003776021.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 304 Score = 170 bits (431), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 105/298 (35%), Positives = 168/298 (56%), Gaps = 10/298 (3%) Query: 3 LQSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEE 62 +Q + F E G+ +AAAE L T + VSR++SRLE +LG L RTTR++ LT E Sbjct: 7 VQLGSIELFCKAAELGSFTAAAEALGLTPASVSRSISRLESRLGVRLFARTTRQIRLTRE 66 Query: 63 GRAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDI 122 G + +Q + L + A+ + + + P G LR+ P+ + ++P + +F+ AYP I Sbjct: 67 GELYRDQCQQALEQIADAERILTGQQKVPSGLLRISVGTPYAHYRLLPLLPRFQQAYPQI 126 Query: 123 ELELTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPESV 182 E+EL+ +R +D VE D+AIR+G DS L A L A L + A+PAYLAR G+P S+ Sbjct: 127 EVELSIANRVVDFVEEGYDLAIRLGVPPDSRLIAHTLEEATLGLFAAPAYLARRGQPRSI 186 Query: 183 EALEQHQLIGFSQPD--QLNHWPLRHSAGD----RYQTRPAIRASSGETIRHLALQGQGI 236 LEQH I F P + W + + G +++R I + + A+ G G+ Sbjct: 187 AELEQHDCIQFILPSSGRAMPWIFKDAQGRDMDFHFKSRQRIHDDVLGCV-NWAIAGGGL 245 Query: 237 ACLSNFMSDADIAAGRLVRLLPQADSGYR-QKISAVYYYSTQLSRRISCFLDFLQEKL 293 + +F++DA + G LV +L QA GYR ++ S +Y ++ LS R+ F++FL++ + Sbjct: 246 FQIYHFIADAAVRRGELVEVLQQA--GYRTRRFSILYPHNRHLSARVRAFVEFLRQAV 301 >gi|300312334|ref|YP_003776426.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 164 bits (414), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 106/288 (36%), Positives = 154/288 (53%), Gaps = 2/288 (0%) Query: 4 QSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEEG 63 Q +++ TF V G+LSAAA + + R L LE++LG LL RTTR++ LT EG Sbjct: 3 QFKQISTFAEVAARGSLSAAARAEGIAPAMIGRRLDALEERLGVKLLQRTTRKIVLTNEG 62 Query: 64 RAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDIE 123 AFLE + ILA +E A+ ++ R+ + G+L + A A F V P + F A + D+ Sbjct: 63 AAFLEDCQRILADLEDAESAVSERSARASGQLLISAPAGFGRQHVAPLLPSFLAEHRDVT 122 Query: 124 LELTTNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPESVE 183 L L NDR D++ D+AIRI L DS+L + L+ +VA+PAYL RHG P+S + Sbjct: 123 LTLNLNDRIADVIGEGIDVAIRIATLTDSNLVSVKLAENHRVIVAAPAYLRRHGTPQSPD 182 Query: 184 ALEQHQLIGFSQPDQLNHWPLRHSAG-DRYQTRPAIRASSGETIRHLALQGQGIACLSNF 242 L +H + S W LR S Y+ + + GE + + AL G+G+A S + Sbjct: 183 ELMRHNCLAMSSEGSQRGWTLRQSGKVSVYKVGGNMVCNDGEVLHNWALAGRGLAWRSMW 242 Query: 243 MSDADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQ 290 A I AG LV +L + + I AV+ L RI F+DFL+ Sbjct: 243 EVGAAIEAGELVTVLDKF-AAPAPSIYAVFAQRRHLPLRIRAFVDFLR 289 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 777,462 Number of extensions: 29623 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 76 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 76 Length of query: 293 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 209 Effective length of database: 1,222,130 Effective search space: 255425170 Effective search space used: 255425170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)