BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0123.p (1938 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312243|ref|YP_003776335.1| exported extracellular alpha-he... 3950 0.0 >gi|300312243|ref|YP_003776335.1| exported extracellular alpha-helical protein [Herbaspirillum seropedicae SmR1] Length = 1938 Score = 3950 bits (10245), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1928/1938 (99%), Positives = 1933/1938 (99%) Query: 1 MHAAALAFALGATMSLLPVKPAQAASATVQSFSPQGEVQRIRQVRARFSESMVKFGDPKA 60 MHAAALAFALGATMSLLPVKPAQAASATVQSFSPQGEVQRIRQVRARFSESMVKFGDPKA Sbjct: 1 MHAAALAFALGATMSLLPVKPAQAASATVQSFSPQGEVQRIRQVRARFSESMVKFGDPKA 60 Query: 61 ASPFDADCAVAGTARWADDKNWVYDFERDLPPGTRCSFTLRAEAKSVAGNSVGGKTRFQF 120 ASPFDADCAVAGTARWADD+NWVYDFERDLPPGTRCSFTLRAEAKSVAGNSVGGKTRFQF Sbjct: 61 ASPFDADCAVAGTARWADDRNWVYDFERDLPPGTRCSFTLRAEAKSVAGNSVGGKTRFQF 120 Query: 121 STGGPAVSRVQPYSGAQIAEDQAFILFQNGPATEPSVRQHLYCEAEGINERIPAKAVGEA 180 STGGPAVSRVQPYSGAQIAEDQAFILFQNGPATEPSVRQHLYCEAEGINERIPAKAVGEA Sbjct: 121 STGGPAVSRVQPYSGAQIAEDQAFILFQNGPATEPSVRQHLYCEAEGINERIPAKAVGEA 180 Query: 181 DRKALLQQFAKGVDPASVTIVQCQQRLPNEAHVKLVWDRGIAAANAPAVVTSQPQVFNFQ 240 DRKALLQQFAKGVDPASVTIVQCQQRLPNEAHVKLVWDRGIAAANAPAVVTSQPQVFNFQ Sbjct: 181 DRKALLQQFAKGVDPASVTIVQCQQRLPNEAHVKLVWDRGIAAANAPAVVTSQPQVFNFQ 240 Query: 241 VRSEFAASLSCQRENANAACSPILPVTLSFSAPVPRKLAEQVVMNATTGKIKPTLEGDKD 300 VRSEFAASLSCQRENANAACSPILPVTLSFSAPVPRKLAEQVVMNATTGKIKPTLEGDKD Sbjct: 241 VRSEFAASLSCQRENANAACSPILPVTLSFSAPVPRKLAEQVVMNATTGKIKPTLEGDKD 300 Query: 301 EAVRELRFKPPFPEKSDFTISLPSGFKDEDGRTLANAGAFPLKSALADFPPLAKFPAAPF 360 EAVRELRFKPPFPEKSDFTISLPSGFKDEDGRTLANAGAFPLKSALADFPPLAKFPAAPF Sbjct: 301 EAVRELRFKPPFPEKSDFTISLPSGFKDEDGRTLANAGAFPLKSALADFPPLAKFPAAPF 360 Query: 361 GIIELNADPTLPVTLRRVEAGLQVRGKDGRAMPGQVADLKVNGDKAVIAWLTRLNKYHEK 420 GIIELNADPTLPVTLRRVEAGLQVRGKDGRAMPGQVADLKVNGDKAVIAWLTRLNKYHEK Sbjct: 361 GIIELNADPTLPVTLRRVEAGLQVRGKDGRAMPGQVADLKVNGDKAVIAWLTRLNKYHEK 420 Query: 421 WGDPKKVDTRSVSLLAKESGAKKLDLPAQKDAKDGEWPFEVIGIPLKDPGFYVVELESQK 480 WGDPKKVDTRSVSLLAKE GAKKLDLPAQKDAK+GEWPFEVIGIPLKDPGFYVVELESQK Sbjct: 421 WGDPKKVDTRSVSLLAKEPGAKKLDLPAQKDAKEGEWPFEVIGIPLKDPGFYVVELESQK 480 Query: 481 LGASLLGKPQPMYVRTSALVTNLSVHVKLGRANGAVWVTRLDDAKPVADAEIRVSNCDGA 540 LGASLLGKPQPMYVRTSALVTNLSVHVKLGRANGAVWVTRLDDAKPVADAEIRVSNCDGA Sbjct: 481 LGASLLGKPQPMYVRTSALVTNLSVHVKLGRANGAVWVTRLDDAKPVADAEIRVSNCDGA 540 Query: 541 QIWQGKTNASGVAMMPALEDACANRGYSPDNADKINGLFVSARKTDEKGRQDMAFALTSW 600 QIWQGKTNASGVAMMPALEDACANRGYSPDNADKINGLFVSARKTDEKGRQDMAFALTSW Sbjct: 541 QIWQGKTNASGVAMMPALEDACANRGYSPDNADKINGLFVSARKTDEKGRQDMAFALTSW 600 Query: 601 NEGIESWRFNLPTDMDKSATVRATTVFDRTLFRAGETVSMKHVIRTETLQGFGLMRKEDL 660 NEGIESWRFNLPTDMDKSATVRATTVFDRTLFRAGETVSMKHVIRTETLQGFGLMRKEDL Sbjct: 601 NEGIESWRFNLPTDMDKSATVRATTVFDRTLFRAGETVSMKHVIRTETLQGFGLMRKEDL 660 Query: 661 PTRVRISHLGSGQQYQFPLTWRGNKSAETTFVLPKQAKLGSYEVVLDQGKVDANASAEPP 720 PTRVRISHLGSGQQYQFPLTWRGNKSAETTFVLPKQAKLGSYEVVLDQGKVDANASAEP Sbjct: 661 PTRVRISHLGSGQQYQFPLTWRGNKSAETTFVLPKQAKLGSYEVVLDQGKVDANASAEPT 720 Query: 721 EQSDDGDYEGGYGRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYING 780 EQSDDGDYEGGYGRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYING Sbjct: 721 EQSDDGDYEGGYGRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYING 780 Query: 781 GAAANQAVQVSSLLRSRSLNIAGYDGFSFDAPRSDEDSNADDQKIVADKLAITLDKNGAG 840 GAAANQAVQVSSLLRSRSLNIAGYDGFSFDAPRSD+DSNADDQKIVADKLAITLDKNGAG Sbjct: 781 GAAANQAVQVSSLLRSRSLNIAGYDGFSFDAPRSDDDSNADDQKIVADKLAITLDKNGAG 840 Query: 841 RATISKLPALQRPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLT 900 RATISKLPALQRPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLT Sbjct: 841 RATISKLPALQRPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLT 900 Query: 901 LTAVTLNTAGQPQAGVPVEIRGTARKTNSHRKRMVGGFYAYENDETKQDLGKLCSGKSDQ 960 LTAVTLNTAGQPQAGVPVEIRG ARKTNSHRKRMVGGFYAYENDE+KQDLGKLCSGKSDQ Sbjct: 901 LTAVTLNTAGQPQAGVPVEIRGIARKTNSHRKRMVGGFYAYENDESKQDLGKLCSGKSDQ 960 Query: 961 HGMFTCDTELSEAGNVELIASAKDGNGNAAAARSSVWVTGRGEVWFDGENQDRIDILPEK 1020 HGMFTCDTELSEAGNVELIASAKDGNGNAAAARSSVWVTGRGEVWFDGENQDRIDILPEK Sbjct: 961 HGMFTCDTELSEAGNVELIASAKDGNGNAAAARSSVWVTGRGEVWFDGENQDRIDILPEK 1020 Query: 1021 KNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQDPTISVPMKAEYGPNVYV 1080 KNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQDPTISVPMKAEYGPNVYV Sbjct: 1021 KNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQDPTISVPMKAEYGPNVYV 1080 Query: 1081 SVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIVDLSKPAWKFGIAEVNVGD 1140 SVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIVDLSKPAWKFGIAEVNVGD Sbjct: 1081 SVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIVDLSKPAWKFGIAEVNVGD 1140 Query: 1141 AGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFALAAVDEALLELQPNGSWD 1200 AGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFALAAVDEALLELQPNGSWD Sbjct: 1141 AGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFALAAVDEALLELQPNGSWD 1200 Query: 1201 VLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGGGGRAPTRELFDTLLVWKPNVTL 1260 VLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGGGGRAPTRELFDTLLVWKPNVTL Sbjct: 1201 VLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGGGGRAPTRELFDTLLVWKPNVTL 1260 Query: 1261 DAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTGSISIRSTQDVQVISGLPPLVREGDS 1320 DAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTGSISIRSTQDVQVISGLPPLVREGDS Sbjct: 1261 DAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTGSISIRSTQDVQVISGLPPLVREGDS 1320 Query: 1321 FNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLPEREVKIAAGQSAEVMWPVQTPEGIN 1380 FNGAVTVRNTTTHDMTIKVAARAQGGTA STLTLPEREVKIAAGQSAEVMWPVQTPEGIN Sbjct: 1321 FNGAVTVRNTTTHDMTIKVAARAQGGTAASTLTLPEREVKIAAGQSAEVMWPVQTPEGIN 1380 Query: 1381 QLAWEISAQEQGGQKARDAMKFTQRVVPAVPVTVQQATLFQLDKNAALDVARPADSLPGR 1440 QLAWEISAQEQGGQKARDAMKFTQRVVPAVPVTVQQATLFQLDKNAALDVARPADSLPGR Sbjct: 1381 QLAWEISAQEQGGQKARDAMKFTQRVVPAVPVTVQQATLFQLDKNAALDVARPADSLPGR 1440 Query: 1441 GGLAISLKPSLAGGSDALTRYFVNYPFACLEQKTSKAIGLRDEAAWQKIVADLPTYLDGD 1500 GGLAISLKPSLAGGSDALTRYFVNYPFACLEQKTSKAIGLRDEAAWQKIVADLPTYLDGD Sbjct: 1441 GGLAISLKPSLAGGSDALTRYFVNYPFACLEQKTSKAIGLRDEAAWQKIVADLPTYLDGD 1500 Query: 1501 GLAYYFPPSDSGARRGSDTLTAYLLAATNEAGYAIPQASRDKMLDGLAAFVEGKVMRDFW 1560 GLAYYFPPSDSGARRGSDTLTAYLLAATNEAGYAIPQASRDKMLDGLAAFVEGKVMRDFW Sbjct: 1501 GLAYYFPPSDSGARRGSDTLTAYLLAATNEAGYAIPQASRDKMLDGLAAFVEGKVMRDFW 1560 Query: 1561 SPQKDLDVRKLAALEALSRYNRVQPAMLGSLQINPNVWPTSGLLDWIAILQRVPAIPDRQ 1620 SPQKDLDVRKLAALEALSRYNRVQPAMLGSLQINPNVWPTSGLLDWI ILQRVPAIPDRQ Sbjct: 1561 SPQKDLDVRKLAALEALSRYNRVQPAMLGSLQINPNVWPTSGLLDWIGILQRVPAIPDRQ 1620 Query: 1621 KKLDEADQILRARLNFQGTRMGFSNEEGDYWWWLMSSGDANANRLILLELNNPAWREDMP 1680 KKLDEADQILRARLNFQGTRMGFSNEEGDYWWWLMSSGDANANRLILLELNNPAWREDMP Sbjct: 1621 KKLDEADQILRARLNFQGTRMGFSNEEGDYWWWLMSSGDANANRLILLELNNPAWREDMP 1680 Query: 1681 RLIAGAIGRQQRGHWSTTTANVWGSLALEKFARKFESEPVTGATRATLEQNGASAATQSL 1740 RLIAGAIGRQQRGHWSTTTANVWGSLALEKFARKFESEPVTGATRATLEQNGASAATQSL Sbjct: 1681 RLIAGAIGRQQRGHWSTTTANVWGSLALEKFARKFESEPVTGATRATLEQNGASAATQSL 1740 Query: 1741 AWSGAPNGGKLQLPWPEGNAQGGSVRLAQEGNGKPWVTLQSLAAVPLKQPFAAGYRITRK 1800 AWSGAPNGGKLQLPWPEGNAQGGSVRLAQEGNGKPWVTLQSLAAVPLKQPFAAGYRITRK Sbjct: 1741 AWSGAPNGGKLQLPWPEGNAQGGSVRLAQEGNGKPWVTLQSLAAVPLKQPFAAGYRITRK 1800 Query: 1801 VEAIEQKQAGVYTRGDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLGRDSAIAQGK 1860 VEAIEQKQAGVYTRGDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLGRDSAIAQGK Sbjct: 1801 VEAIEQKQAGVYTRGDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLGRDSAIAQGK 1860 Query: 1861 QDAPRERGMGWLAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFNLPPTRVEAMYA 1920 QDAPRERGMGWLAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFN+PPTRVEAMYA Sbjct: 1861 QDAPRERGMGWLAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFNMPPTRVEAMYA 1920 Query: 1921 PEMFGEVPNGKLVVKAAQ 1938 PEMFGEVPNGKLVVKAAQ Sbjct: 1921 PEMFGEVPNGKLVVKAAQ 1938 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 6,123,292 Number of extensions: 265139 Number of successful extensions: 755 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 755 Number of HSP's successfully gapped: 1 Length of query: 1938 Length of database: 1,619,870 Length adjustment: 100 Effective length of query: 1838 Effective length of database: 1,146,370 Effective search space: 2107028060 Effective search space used: 2107028060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 138 (57.8 bits)