BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0115.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 763 0.0 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 119 4e-28 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 103 2e-23 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 99 5e-22 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 92 9e-20 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 763 bits (1970), Expect = 0.0, Method: Compositional matrix adjust. Identities = 384/385 (99%), Positives = 385/385 (100%) Query: 1 MQFNTMLKTIPALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINA 60 MQFNTMLKTIPALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINA Sbjct: 1 MQFNTMLKTIPALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINA 60 Query: 61 KGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKV 120 KGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKV Sbjct: 61 KGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKV 120 Query: 121 VVFGTSNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSG 180 VVFGTSNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSG Sbjct: 121 VVFGTSNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSG 180 Query: 181 YDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTR 240 YDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTR Sbjct: 181 YDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTR 240 Query: 241 ALGMKQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAKFGADP 300 ALGMKQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAKFGADP Sbjct: 241 ALGMKQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAKFGADP 300 Query: 301 DQFAAQAYDAMYILADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRAAPAVAG 360 DQFAAQAYDAMYI+ADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRAAPAVAG Sbjct: 301 DQFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRAAPAVAG 360 Query: 361 KQVGFDADQEAIVNIAKGGKFVLLK 385 KQVGFDADQEAIVNIAKGGKFVLLK Sbjct: 361 KQVGFDADQEAIVNIAKGGKFVLLK 385 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 106/360 (29%), Positives = 164/360 (45%), Gaps = 18/360 (5%) Query: 1 MQFNTMLKTIPALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINA 60 M + T AL+ A SQAA IK+GV+ TG +A GV++++G LA EINA Sbjct: 1 MTYKTQAFVAAALMSLALTSQAA--DPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINA 58 Query: 61 KGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKV 120 GG+ G ++ L+ D++ K E + + ++LI ++KV+A G + A A+ AK+ Sbjct: 59 AGGLLGRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKI 118 Query: 121 VVFGTSNTADGITAM-----GPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDA 175 V T IT + FR S + + AV KVA++ + Sbjct: 119 PVINNVATGTVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAIL-ADSTN 177 Query: 176 FTKSGYDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANI 235 + + G + + L +K+ E Y DVD AQL K K G A++ + E A I Sbjct: 178 YGQLGREDLEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQI 237 Query: 236 ILQTRALGMKQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAK 295 L P IG L+ + + G+ M + + TP KAFI AY+ Sbjct: 238 ANGMEKLNWHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFIQDGNTPKRKAFIEAYQKA 297 Query: 296 FGAD--PDQF-AAQAYDAMYILADALKNVKLSGNLAKDRDALRAALPAV--KIDGATGKF 350 + D P AAQ YD++ +LA A+K + D D +RAAL + K++G + Sbjct: 298 YKVDRMPSAVSAAQGYDSILLLAAAIKQAGTT-----DGDKVRAALENLNTKVEGVVTTY 352 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 93/303 (30%), Positives = 145/303 (47%), Gaps = 11/303 (3%) Query: 25 AADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKLALVIEDEQGKKEEAI 84 A +K+ +A +TG A+YG +K G A E+INA GG NG+K V+ D+ + ++A+ Sbjct: 26 AETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAV 85 Query: 85 NVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGITAMGP--FTFRN 142 V K++ Q + V G S S A I VV+ S TA +T P F FR Sbjct: 86 AVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRT 144 Query: 143 SVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKATLEHQKIPVTTTEAYA 202 + P + ++ KKVAV++ + ++ + KA L+ K+PV E Sbjct: 145 IGRDDQQGPAAARYVIEKLKPKKVAVLH-DKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 203 KGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGNGLNSPKLFEI 262 GD D+ A +TK+K+ D + E I+ Q R G+K F+G G+ + + I Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAI 263 Query: 263 AKDAGDNTLMGSP--WSAENQTPANKAFITAYKAKFGADPD-QFAAQAYDAMYILADALK 319 A A + L+ P ++A+ PAN A + A DP+ F AY + I+ADA+ Sbjct: 264 AGPASEGMLVTLPADFAAD---PANAA-LVKAFAAAKRDPNGPFQMPAYSGVKIIADAIA 319 Query: 320 NVK 322 K Sbjct: 320 GAK 322 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 99.0 bits (245), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 96/334 (28%), Positives = 150/334 (44%), Gaps = 17/334 (5%) Query: 28 IKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKG-GVNGDKLA--LVIEDEQGKKEEAI 84 +K+G + LTGP A G + G +A E +NA+ V G K+ ++ ED+Q + + Sbjct: 30 VKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITVGGKKIKFDVIAEDDQADPKSGV 89 Query: 85 NVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGITAMGPFT-FRNS 143 V +KL Q V A+ GP S A + N A +VV T + IT G T FR + Sbjct: 90 AVAQKLADQG-VKAIVGPYNSGVTIPASRVYNDAGIVV-ATVASNPKITQQGFATLFRVA 147 Query: 144 VMEADVLPVTVKAAVKHFGIKKVAVIYGNDD--AFTKSGYDVFKATLEHQKIPVTTTEAY 201 ++ + A K K+VAVI DD A+ + F + I V +T+ Sbjct: 148 ASDSQLGGKMALYAAKELKFKRVAVI---DDRTAYGQGLAQEFIKVAKANGIDVVSTDFT 204 Query: 202 AKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGNGLNSPKLFE 261 DF A LT IK PDA+ A + I Q + LG+ P +GG+G+ SP++ Sbjct: 205 NDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLGVDVPLMGGDGICSPEMGR 264 Query: 262 IAKDA-GDNTLMGSPWSAENQTPANKAFITAYKAKFGADPDQFAAQAYDAMYILADALKN 320 + DA G++ + ++ K F Y+ K+ + +A YD M ++A A+K Sbjct: 265 LGGDAIGESVYCTQGGTMLDKAKEGKVFSDEYQKKYNRPAETYAVSFYDGMMLIAQAMKQ 324 Query: 321 VKLSGNLAKDRDALRAALPAVKIDGATGKFAFRA 354 + D AL + G G++ F A Sbjct: 325 AN-----SVDPKQFGPALAKISYKGVAGQYDFDA 353 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 91.7 bits (226), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 107/360 (29%), Positives = 162/360 (45%), Gaps = 42/360 (11%) Query: 12 ALLIGATLSQAALAADI-KLGVAEALTGPAAKYGVAIKNGFTLASEEI-NAKGGVNGDKL 69 A+ +GA+L A AAD+ K+G L+GPA+ GV + GF LA + + N GGV + Sbjct: 14 AMAVGASLP--AQAADVVKVGFLTTLSGPASAPGVDEREGFMLALKTLGNKLGGVPAE-- 69 Query: 70 ALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTA 129 +++ D+Q + A +LI ++KV + G SN A P A A+K VV + Sbjct: 70 -VIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAAFASKTVVVSANAGP 128 Query: 130 DGITAMG--PFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKAT 187 + G P+ F S + D + G ++VA+I N A K FK Sbjct: 129 AQLAGAGCNPYFFSAS-YQNDAAHEAAGKLMADKGYQRVAIIAPNYPA-GKDASAGFKRF 186 Query: 188 LEHQKIPVTTTEAYAK-GDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQ 246 L +K P E + K G +DF ++ +++A N DA+ N + Q A GM Sbjct: 187 L--KKDP--AVELFPKLGQLDFATEIAQLRAANIDALYFFLPGGMGVNFVKQYAAAGM-- 240 Query: 247 PFIGGNGLNSPKLFEI--AKDAGDNTLMGS----------PWSAENQTPANKAFITAYKA 294 PK+ + A DA L WS++ N+AF+ AY Sbjct: 241 ---------DPKIVRVMPAYDADQQMLSNGDLIAGVANTGQWSSDLPNEQNRAFVAAYLK 291 Query: 295 KFGADPDQFAAQAYDAMYILADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRA 354 ++G P FA+QAYDA ++ A+K V G ++R+AL AA+ G F F A Sbjct: 292 EYGRQPSMFASQAYDAAMLIDSAVKAV---GGKVENREALIAAIKTANFKSVRGGFRFAA 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,106,106 Number of extensions: 43829 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 18 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)