BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0112.p
         (312 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312231|ref|YP_003776323.1| dienelactone hydrolase [Herbasp...   152   4e-38
gi|300312410|ref|YP_003776502.1| dienelactone hydrolase [Herbasp...    92   5e-20

>gi|300312231|ref|YP_003776323.1| dienelactone hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 257

 Score =  152 bits (383), Expect = 4e-38,   Method: Compositional matrix adjust.
 Identities = 95/98 (96%), Positives = 95/98 (96%)

Query: 1   MRIWGTPLATFCAAAFMLAASALTHAAPNSAPPAPLEARRVALTSVDGNGGKATSLYGVW 60
           MRIWGTPLATFCAAAFMLAASALTHAAPNSAPPAPLEARRVALTSVDGNGGKATSLYGVW
Sbjct: 29  MRIWGTPLATFCAAAFMLAASALTHAAPNSAPPAPLEARRVALTSVDGNGGKATSLYGVW 88

Query: 61  IKARKSLNSKGPYPTVIALHGCGGLYSIVKDGRGEFTP 98
           IKARKSLNSKGPYPTVIALHGCGGLYSIVKDGRG   P
Sbjct: 89  IKARKSLNSKGPYPTVIALHGCGGLYSIVKDGRGGIHP 126


>gi|300312410|ref|YP_003776502.1| dienelactone hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 345

 Score = 92.0 bits (227), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 71/223 (31%), Positives = 97/223 (43%), Gaps = 27/223 (12%)

Query: 71  GPYPTVIALHGCGGLYSIVKDG--------------RGEFTPRHLAMARVLTDAGYNVLF 116
           GP+P V+ LHG  G YS   +G                  + RH A A    + GY  L 
Sbjct: 95  GPHPAVVMLHGRAGPYSSRINGDCTRIGPALPSPCDASTLSQRHQAWAAYWIERGYLALL 154

Query: 117 PDSFTPRG-RRSTCQDTVAQREISAMN----RRFDVQAAMRWVTTQPDVDVKRIALLGWS 171
            DS+ PRG      + T    E + +N    R  D + A+ W+  + DV+  RI L GWS
Sbjct: 155 VDSYGPRGVAHGFGRHTHGAPERAEVNELTVRPLDAEGALAWLAPRSDVNPARILLQGWS 214

Query: 172 HGASTVLASLNLAETDVAVRKIQPRAAVAFYPDCRPYAKPSEPFKPAAPLLILMGENDDW 231
           +GAST L  +    + +         A+ FYP C P A  S   +   P+ +L+G  D  
Sbjct: 215 NGASTALNVMQRQASRLGGTAPSFAGAMVFYPGCGPQALLSSSPELDRPVQVLLGAADQE 274

Query: 232 TPPQAC-----EAIEQKMQGSDTELALRLYPDTYHDFDAPGLP 269
             PQ C     +AI  +   S     + LYP   HDFD PG P
Sbjct: 275 VSPQRCLRVLRQAIPMRRNSSPV---VTLYPGATHDFDDPGRP 314


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.134    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 996,868
Number of extensions: 41968
Number of successful extensions: 125
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 121
Number of HSP's successfully gapped: 2
Length of query: 312
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 227
Effective length of database: 1,217,395
Effective search space: 276348665
Effective search space used: 276348665
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)