BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0105.p (562 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 1095 0.0 gi|300313617|ref|YP_003777709.1| translation elongation factor [... 175 1e-44 gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 156 5e-39 gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 96 1e-20 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 82 2e-16 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 1095 bits (2831), Expect = 0.0, Method: Compositional matrix adjust. Identities = 529/530 (99%), Positives = 530/530 (100%) Query: 33 LIAREVKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEK 92 +IAREVKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEK Sbjct: 1 MIAREVKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEK 60 Query: 93 QRGISVASSVMQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTI 152 QRGISVASSVMQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTI Sbjct: 61 QRGISVASSVMQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTI 120 Query: 153 KLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGVYH 212 KLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGVYH Sbjct: 121 KLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGVYH 180 Query: 213 LLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIEQLKMEVELVHGASHPFNLDD 272 LLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIEQLKMEVELVHGASHPFNLDD Sbjct: 181 LLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIEQLKMEVELVHGASHPFNLDD 240 Query: 273 FLAGVQTPVFFGSAINNFGVREILDALLDWAPGPGGRDATVREVKPTEEPFSGFVFKIQA 332 FLAGVQTPVFFGSAINNFGVREILDALLDWAPGPGGRDATVREVKPTEEPFSGFVFKIQA Sbjct: 241 FLAGVQTPVFFGSAINNFGVREILDALLDWAPGPGGRDATVREVKPTEEPFSGFVFKIQA 300 Query: 333 NMDPAHRDRIAFLRVCSGRFERGMKLKHLRLNRDIKVSSVVTFMASSREQVEEAYAGDII 392 NMDPAHRDRIAFLRVCSGRFERGMKLKHLRLNRDIKVSSVVTFMASSREQVEEAYAGDII Sbjct: 301 NMDPAHRDRIAFLRVCSGRFERGMKLKHLRLNRDIKVSSVVTFMASSREQVEEAYAGDII 360 Query: 393 GLPNHGNMQIGDSFSEGEMLQFTGIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEGAV 452 GLPNHGNMQIGDSFSEGEMLQFTGIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEGAV Sbjct: 361 GLPNHGNMQIGDSFSEGEMLQFTGIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEGAV 420 Query: 453 QVFKPVTGSDLILGAVGVLQFEVVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADF 512 QVFKPVTGSDLILGAVGVLQFEVVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADF Sbjct: 421 QVFKPVTGSDLILGAVGVLQFEVVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADF 480 Query: 513 EKSSAGANLAIDAAGNMAYLASSGVNLRLTQERWPEITFHATREHAAKLD 562 EKSSAGANLAIDAAGNMAYLASSGVNLRLTQERWPEITFHATREHAAKLD Sbjct: 481 EKSSAGANLAIDAAGNMAYLASSGVNLRLTQERWPEITFHATREHAAKLD 530 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 175 bits (443), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 139/524 (26%), Positives = 239/524 (45%), Gaps = 64/524 (12%) Query: 38 VKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGIS 97 ++ R GI +H DAGKTT TE++L ++G G V AT DWME E++RGI+ Sbjct: 7 IENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEV----HNGAATMDWMEQEQERGIT 62 Query: 98 VASSV-------MQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQ 150 + S+ M Y +H +N++DTPGH DF+ + R + +D A+MV D+ GV+ Q Sbjct: 63 ITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQ 122 Query: 151 TIKLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGV 210 + + P I F+NK+DR D + ++E LK + P+ P+G +F GV Sbjct: 123 SETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFSGV 182 Query: 211 YHLLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIE---------------QLK 255 L++ + +++ +E + G + + I PLE+E + Sbjct: 183 IDLVKMKAIIW---DEASQGVLFKYEDI---------PLELEDTARHWRDLMVEQAAEAN 230 Query: 256 MEVELVHGASHPFNLDDFLAGVQ--------TPVFFGSAINNFGVREILDALLDWAPGP- 306 E+ + + +P DD G++ P+ GSA N GV+ +LDA++D+ P P Sbjct: 231 EELLEKYLSGNPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPV 290 Query: 307 --------GGRDATVREVKPTEEPFSGFVFKIQANMDPAHRDRIAFLRVCSGRFERGMKL 358 G D+ + EEPFS FKI DP ++ F RV SG G + Sbjct: 291 DVPAIAGHGEDDSEIERHPSDEEPFSALAFKIMT--DP-FVGQLTFFRVYSGIVNSGDTV 347 Query: 359 KHLRLNRDIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFSEGE---MLQFT 415 + + ++ ++ A+ R++++E +AGDI ++ GD+ S+ E +L+ Sbjct: 348 YNPVKGKKERLGRILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERM 407 Query: 416 GIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEG-AVQVFKPVTGSDLILGAVGVLQFE 474 P P + + +++ L +L +E + +V I+ +G L E Sbjct: 408 IFP--EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLE 465 Query: 475 VVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADFEKSSAG 518 ++ R+ E+ V+A ++ I + F K S G Sbjct: 466 ILVDRMKREFNVEATVGKPQVAYREAIRKAVEDVEGKFVKQSGG 509 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 156 bits (394), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 130/518 (25%), Positives = 230/518 (44%), Gaps = 52/518 (10%) Query: 38 VKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGIS 97 ++R R GI +H DAGKTT TE++L ++G G V AT DWME E++RGI+ Sbjct: 7 IERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEV----HDGAATMDWMEQEQERGIT 62 Query: 98 VASSV-------MQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQ 150 + S+ M + H +N++DTPGH DF+ + R + +D A MV A GV+ Q Sbjct: 63 ITSAATTCYWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQ 122 Query: 151 TIKLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGV 210 + + P + F+NK+DR + ++ +++ + LK P+ PIG +NF GV Sbjct: 123 SETVWRQANKYKVPRLAFVNKMDRTGANFFKVYEQMRARLKANPIPMQVPIGAEENFEGV 182 Query: 211 YHLL------------------RDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIE 252 L+ RD KA ++ N+ + L + +E Sbjct: 183 IDLVKMRAIYWDDASQGMKFDYRDIPEHLKADAQKWRENLVETAAEASEDLMNKY---LE 239 Query: 253 QLKMEVELVHGASHPFNLDDFLAGVQTPVFFGSAINNFGVREILDALLDWAPGP------ 306 + + + GA ++G P+ G+A N GV+ +LD ++++ P P Sbjct: 240 EGDLTEAEIKGAIR----QRTISGEIVPMMCGTAFKNKGVQAMLDGVVEYLPSPVDIPPV 295 Query: 307 ----GGRDATVREVKPTEEPFSGFVFKIQANMDPAHRDRIAFLRVCSGRFERGMKLKHLR 362 + VRE K +E FS FKI DP ++ F+R SG G + + Sbjct: 296 PGLNEDDEPVVREAK-DDEKFSALAFKIAT--DP-FVGQLCFIRCYSGTLNSGDTVLNSV 351 Query: 363 LNRDIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFSEGE-MLQFTGIPYFA 421 ++ ++ +V A+ RE+++E AGDI + + GD+ + + M+ + + Sbjct: 352 KSKKERIGRIVQMHANQREEIKEMMAGDIAAVVGLKDTTTGDTLCDDKAMVVLERMVFPE 411 Query: 422 PDFFRVARIRNPLKIKQLHKGLQQL-GEEGAVQVFKPVTGSDLILGAVGVLQFEVVASRL 480 P + + +++ L +L E+ + +V I+ +G L +++ R+ Sbjct: 412 PVISQAVEPKTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRM 471 Query: 481 LNEYGVDAVFEGTSISSARWISCDDKKKLADFEKSSAG 518 E+ V+A ++ I ++ F K S G Sbjct: 472 KREFNVEATVGKPQVAYRETIRKTCEESEGKFVKQSGG 509 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 95.5 bits (236), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 57/171 (33%), Positives = 92/171 (53%), Gaps = 15/171 (8%) Query: 42 RTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGISVASS 101 R F II+H D GK+TL +++ IQL G + R+ D M+IE++RGI++ + Sbjct: 5 RNFSIIAHIDHGKSTLADRI------IQLCGGLSNREMEAQVL-DSMDIERERGITIKAQ 57 Query: 102 VMQFEYRD-----HVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLN 156 Y+ + +NL+DTPGH DFS + R L+A + AL+V+DA++GVE QT+ Sbjct: 58 TAALSYKAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCY 117 Query: 157 VCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCA---PVTWPIGMG 204 ++ +NK+D + D E+E V+ I + P + G+G Sbjct: 118 TALDLGVEVVPVLNKIDLPSADPDNAKSEIEDVIGIDASDATPCSAKTGLG 168 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 55/162 (33%), Positives = 81/162 (50%), Gaps = 13/162 (8%) Query: 49 HPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGISVASSVMQFEYR 108 H D GKTTL ++LL SG + V R D +IEK+RGI++ S EY+ Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDNR------VMDSNDIEKERGITILSKNCAVEYK 83 Query: 109 DHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLNVCRMRNTPIITF 168 +N++DTPGH DF + RVL+ VDS L+++DA +G QT + I Sbjct: 84 GTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVV 143 Query: 169 MNKLDRE-------TRDSLELLDELESVLKIQCAPVTWPIGM 203 +NK+DR + EL D+L + + PV + G+ Sbjct: 144 LNKIDRPGARAEWAINATFELFDKLGATEEQLDFPVIYASGL 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,618,752 Number of extensions: 65218 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 8 Length of query: 562 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 472 Effective length of database: 1,193,720 Effective search space: 563435840 Effective search space used: 563435840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)