BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0101.p (535 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis tra... 1043 0.0 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 649 0.0 gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 486 e-138 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 386 e-108 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 379 e-106 >gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 1043 bits (2697), Expect = 0.0, Method: Compositional matrix adjust. Identities = 531/535 (99%), Positives = 531/535 (99%) Query: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI Sbjct: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 Query: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDADRKLLEADRQAFAD 120 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDD DRKLLEADRQAFAD Sbjct: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDTDRKLLEADRQAFAD 120 Query: 121 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI 180 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI Sbjct: 121 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI 180 Query: 181 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE 240 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE Sbjct: 181 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE 240 Query: 241 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA 300 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA Sbjct: 241 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA 300 Query: 301 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI 360 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI Sbjct: 301 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI 360 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV Sbjct: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV 420 Query: 421 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA 480 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA Sbjct: 421 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA 480 Query: 481 ALVEEAAAASQSLREQAKGLAQAVSVFRLERSAAAVKPVVAAPVQEQPAMLALTA 535 ALVEEAAAASQSLREQAKGLAQAVSVFRLERSAAA KP V APVQEQPAMLALTA Sbjct: 481 ALVEEAAAASQSLREQAKGLAQAVSVFRLERSAAAAKPAVVAPVQEQPAMLALTA 535 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 649 bits (1674), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/536 (66%), Positives = 424/536 (79%), Gaps = 4/536 (0%) Query: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 MTIA+KL+LL +AV+LG+ +++G+YQS +A +A YS+ +T+PS+LAL A +A +GI Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNGATDAIYGI 90 Query: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDADRKLLEADRQAFAD 120 R+ I +Y +P +R + MA H + FD++ R ISDD DR++LEADR+ FA+ Sbjct: 91 RLNIARYV--IEPTAREKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRFFAE 148 Query: 121 YEKQRAVVLRIGAESRNEEALR-AIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEG 179 YEK R ++ +G+ + E+ALR A+E+ + P G +L+A L EH+ Y EQLGRQG E A+ Sbjct: 149 YEKHRMAMVDLGSAGK-EDALRVAMEEKMAPTGQRLMAVLNEHKNYNEQLGRQGAEAADE 207 Query: 180 ILQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKD 239 LQ S Y I + LV +V A SLL+ R I SL A +A+ I GDLSS++ + G+D Sbjct: 208 HLQLSIYLTIAAATLVIVMVSALSLLLVRNIRHSLHAATELADTIGRGDLSSQIVLRGRD 267 Query: 240 EVGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETAS 299 EV QL+ ++ MT SLV I+G V S A IE+A+ EIASGNLDLS+RTEAQAGS+EET S Sbjct: 268 EVTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQAGSLEETVS 327 Query: 300 AMEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISV 359 AM QLT TVKQNADNA QAD LA SAS VA + GKVV EV+ TM+AIN+SSLKIVDIISV Sbjct: 328 AMGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSLKIVDIISV 387 Query: 360 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEK 419 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSV K Sbjct: 388 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVNK 447 Query: 420 VQSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQN 479 VQSGSRLV+QAG+TME+VVTSV+RVS IVRDISVASGEQSDGIAQVNQAIGMMDQTTQQN Sbjct: 448 VQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQN 507 Query: 480 AALVEEAAAASQSLREQAKGLAQAVSVFRLERSAAAVKPVVAAPVQEQPAMLALTA 535 AALVEEAAAASQSLREQAKGLAQAVSVFRLERSA A +P PVQEQPAMLALTA Sbjct: 508 AALVEEAAAASQSLREQAKGLAQAVSVFRLERSAEAPQPESTEPVQEQPAMLALTA 563 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 486 bits (1251), Expect = e-138, Method: Compositional matrix adjust. Identities = 269/513 (52%), Positives = 366/513 (71%), Gaps = 1/513 (0%) Query: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 ++I +L++L AVI+G L+ + + AS++S N VPSLL+L + G+ Sbjct: 3 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 62 Query: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDADRKLLEADRQAFAD 120 R+ W+Y A T+ V R +E + RV A +Y +E ++D D++LL A R ++ + Sbjct: 63 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 122 Query: 121 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI 180 ++K A L++ + EA RA+ P D+L A+ E R Y E++ + G A+ Sbjct: 123 FDKSLAEALKLANAGQWNEA-RALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQET 181 Query: 181 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE 240 + ++ AIL+S V A+V LLI+R I+ SL +A+ +A IA GDL+ ++ + DE Sbjct: 182 MAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDTSSTDE 241 Query: 241 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA 300 +G L+ A+ +M +LV IV VR V + +ASS+IA+GN+DLSARTE QA S+E+TA+A Sbjct: 242 IGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAAA 301 Query: 301 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI 360 E+LT+TVKQNA+NA QAD LAS+AS +A+EGG+VV++VV TM+ I SSSL I DIISVI Sbjct: 302 TEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISVI 361 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSASAA+EIK+LIDDSV KV Sbjct: 362 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSKV 421 Query: 421 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA 480 ++GS+LVM AGNTM+QVV SV+RV+D+V +IS AS EQS GI QVNQAI MDQ+TQ+NA Sbjct: 422 ENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQENA 481 Query: 481 ALVEEAAAASQSLREQAKGLAQAVSVFRLERSA 513 ALVEEA AA+QSL+EQA+ L +AV+VF+L + A Sbjct: 482 ALVEEATAAAQSLQEQARQLERAVNVFKLRQEA 514 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 386 bits (992), Expect = e-108, Method: Compositional matrix adjust. Identities = 262/520 (50%), Positives = 349/520 (67%), Gaps = 19/520 (3%) Query: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 + IAKKL + AVI+ L L I + + V +A++ + N +P++ A + + Sbjct: 7 LKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASLPRM 66 Query: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYARE-DISDDADRKLLEADRQAFA 119 R+ + A P R KA+ + D + A R A E IS+ ++ L E ++FA Sbjct: 67 RISELQMVTAATPAEREDALKAIKSRLD--ILAKQRAAYEAQISEAEEKVLYEKFSKSFA 124 Query: 120 DYEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDK------LIAALAEHRKYKEQLGRQG 173 Y + + RI AE + EEA RA+ GD +I + E K + ++ Sbjct: 125 TYLEIDKELARISAEGKEEEA-RAL-----FKGDSNKTFRSMIENMDEIIKVNDAGSKRS 178 Query: 174 VETAEGILQSSTYQAILVSLLVAAIVGATSL--LISRGISVSLDDAIRVANAIAHGDLSS 231 + AE + +S+T ++ LL A +V A L +++R +S L++A+ VA +A GDL+ Sbjct: 179 DDMAEQVYRSATLW--IIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTM 236 Query: 232 KVKMTGKDEVGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQA 291 ++ T DE G L+H++ M SL IVG VR I +AS EIA+GNLDLS+RTE QA Sbjct: 237 DIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQA 296 Query: 292 GSIEETASAMEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSL 351 GS+EETASAME+LTATVKQNADNA QA+ LA+SAS +A++GG VV +VV TM+ I SS Sbjct: 297 GSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSN 356 Query: 352 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKE 411 +I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAA+EIK+ Sbjct: 357 RIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQ 416 Query: 412 LIDDSVEKVQSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGM 471 LIDDSVEKV +GS+LV +AG TM +VV SV+RV+D+V +IS AS EQS GI ++N+AI Sbjct: 417 LIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQ 476 Query: 472 MDQTTQQNAALVEEAAAASQSLREQAKGLAQAVSVFRLER 511 MD+ TQQNAALVEEAAAA+QSL++QA L+Q VSVF+L+R Sbjct: 477 MDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKLQR 516 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 379 bits (973), Expect = e-106, Method: Compositional matrix adjust. Identities = 248/504 (49%), Positives = 331/504 (65%), Gaps = 47/504 (9%) Query: 62 VGIWKYAA---ATDPVSRTRLEK---------AMAEQHDRVLA-------AFDRYAREDI 102 +G+W+ + ATD ++R L+K A+ E R A AF Y ++ I Sbjct: 27 LGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKENGVRTFAVMKSDDDAFQAYFQKQI 86 Query: 103 ------------------SDDADRKLLEADRQAFADYEKQRAVVLRIGAESRNEEALRAI 144 +D +++L + + A Y R + + + A Sbjct: 87 DAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREMT 146 Query: 145 EQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGILQS-STYQAILVSLLVAAIV--GA 201 + +V D + + +++Q+ Q TA I + + +++L++L IV A Sbjct: 147 DTTLVKMMDDYADGVLRYADFQKQVIDQ---TAAAINRDYRSGRSMLLALGAIEIVLGAA 203 Query: 202 TSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDEVGQLLHAIGEMTASLVNIVGN 261 + L++R I+ L+ A+ +A +A GDL+S++ + +DE G+LL A+ M +L++IVG Sbjct: 204 LAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGR 263 Query: 262 VRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASAMEQLTATVKQNADNAHQADTL 321 VR I +AS EIA+GNLDLSARTEAQAGS+EETASAMEQLT+TVKQNADNA QA+ L Sbjct: 264 VRMGTETINTASREIATGNLDLSARTEAQAGSLEETASAMEQLTSTVKQNADNARQANEL 323 Query: 322 ASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVIDGIAFQTNILALNAAVEAARA 381 A+SAS VA++GG VV +VV TM AI++SS KI DII VIDGIAFQTNILALNAAVEAARA Sbjct: 324 AASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVIDGIAFQTNILALNAAVEAARA 383 Query: 382 GEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKVQSGSRLVMQAGNTMEQVVTSV 441 GEQGRGFAVVASEVRSLAQRSASAA+EIK+LIDDSVEKV GSRLV +AG+TM++VV SV Sbjct: 384 GEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKVDDGSRLVARAGDTMQEVVQSV 443 Query: 442 QRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNAALVEEAAAASQSLREQAKGLA 501 RV+ +VR+I++AS EQSDGI QVN AI +D+TTQQNAALVE+AAAA++S+++QA L Sbjct: 444 ARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNAALVEQAAAAARSMQDQAASLT 503 Query: 502 QAVSVFRLERSAAAVKPVVAAPVQ 525 AVSVFRLE A V+A VQ Sbjct: 504 DAVSVFRLEDDA----QVLAVAVQ 523 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.125 0.316 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,125,019 Number of extensions: 38838 Number of successful extensions: 515 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 69 Length of query: 535 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 445 Effective length of database: 1,193,720 Effective search space: 531205400 Effective search space used: 531205400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)