BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0097.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleoti... 839 0.0 gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit... 183 3e-47 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 56 5e-09 >gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleotide-disulfide oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 839 bits (2168), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/413 (100%), Positives = 413/413 (100%) Query: 1 METQANKSTARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVL 60 METQANKSTARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVL Sbjct: 1 METQANKSTARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVL 60 Query: 61 AGDKSIDDIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGST 120 AGDKSIDDIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGST Sbjct: 61 AGDKSIDDIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGST 120 Query: 121 PFIIPVPGHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVT 180 PFIIPVPGHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVT Sbjct: 121 PFIIPVPGHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVT 180 Query: 181 VVHVTDALMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPA 240 VVHVTDALMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPA Sbjct: 181 VVHVTDALMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPA 240 Query: 241 DLVVMTAGVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPI 300 DLVVMTAGVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPI Sbjct: 241 DLVVMTAGVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPI 300 Query: 301 WDQARVCGAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDDTEDLIVRDPRRGVY 360 WDQARVCGAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDDTEDLIVRDPRRGVY Sbjct: 301 WDQARVCGAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDDTEDLIVRDPRRGVY 360 Query: 361 KRLVLRGTRLVGAVLYGDVKDGPWYFDLIQSGRDISSFRRELPFGQAMCQQAA 413 KRLVLRGTRLVGAVLYGDVKDGPWYFDLIQSGRDISSFRRELPFGQAMCQQAA Sbjct: 361 KRLVLRGTRLVGAVLYGDVKDGPWYFDLIQSGRDISSFRRELPFGQAMCQQAA 413 >gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 857 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 139/397 (35%), Positives = 211/397 (53%), Gaps = 26/397 (6%) Query: 14 LVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSIDDIMLNP 73 ++V+G+GM G + +E L + +T+ EP Y+R+ LS AG K+ +D+ L Sbjct: 3 IIVIGHGMVGHKFLECLAESGAGDLQVTILCEEPRPAYDRVHLSEFFAG-KTAEDLSLVA 61 Query: 74 RAWYDDN------GITLHAGDPVVHIDRRQRTVH-AKSGLTVQYDRLLLATGSTPFIIPV 126 ++D + L+A + ++R+Q V+ A T+ YD+L+LATGS PF+ V Sbjct: 62 PGFFDRGRSAVQFDLKLNAKAQQIDLERKQVLVNVAGVEETMSYDKLVLATGSYPFVPTV 121 Query: 127 PGHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTD 186 G+Q +R I+D++AML+ + VVIGGGLLGLE A L ++ VV Sbjct: 122 AGNQRKDCFVYRTIEDLEAMLECGSRSKIGVVIGGGLLGLECAKALRDLKLETHVVEFAP 181 Query: 187 ALMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIV-GPDRVTAVRFKDGSSIPADLVVM 245 LM Q+D+ +L++ +E+ G+ +T EIV G + F+DGS + D++V Sbjct: 182 RLMAVQVDESGGRVLRQKIEELGVTAHTQKNTVEIVDGKHHTHRMNFEDGSHLETDMIVF 241 Query: 246 TAGVRPNIALAKAAGLHC--ERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPIWDQ 303 +AG+RP LA+ AGL I +D++ T DP VYA+GEC FGLVAP +D Sbjct: 242 SAGIRPRDELARQAGLKVGDRGGIAIDNSCLTSDPAVYAIGECALWNGKIFGLVAPGYDM 301 Query: 304 ARVCGAHLAGAGHRRYVQQATA-------TKLKVTGVDLYSAGDFIGGD-DTEDLIVRDP 355 AR HL HR + A TKLK+ GVD+ S GD G + + D Sbjct: 302 ARTAAKHL--LSHRDEAIEVPAFNGADMSTKLKLMGVDVASIGDAHGKEAGSRSYQFTDE 359 Query: 356 RRGVYKRLVL--RGTRLVGAVLYGDVKDGPWYFDLIQ 390 R+ +YK++V+ G L+GAV+ GD + Y L+Q Sbjct: 360 RKQIYKKIVVSESGKHLLGAVMVGDASE---YGTLLQ 393 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 56.2 bits (134), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 54/186 (29%), Positives = 85/186 (45%), Gaps = 20/186 (10%) Query: 113 LLLATGSTPFIIPVPGHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGL 172 +L+ATG P +PG +L I D + A+ Q AVV+GGG + +E A+ L Sbjct: 136 ILVATGGRPDKPAIPGAELG--ITSDDFFHLKALPQ------RAVVLGGGYIAVELASIL 187 Query: 173 MRQGMDVTVVHVTDALMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEI-------VGPD 225 G +VT+V+ + L+ + +D L + KG+R + NA E I Sbjct: 188 NGLGCEVTLVYRRERLL-RNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQAGEASEA 246 Query: 226 RVTAVRFKDGSSIPADLVVMTAGVRPNIA---LAKAA-GLHCERAIIVDDTLQTYDPRVY 281 V VR +G ++ A V+ G N A L +A L AI V+ ++ P + Sbjct: 247 SVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVKLKANGAIEVNQDFESSVPSIL 306 Query: 282 AVGECV 287 AVG+ + Sbjct: 307 AVGDVI 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,286,319 Number of extensions: 54799 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 3 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)