BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0094.p
         (435 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite t...   862   0.0  
gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter pro...    81   1e-16

>gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite
           transporter transmembrane protein [Herbaspirillum
           seropedicae SmR1]
          Length = 435

 Score =  862 bits (2226), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 434/435 (99%), Positives = 434/435 (99%)

Query: 1   MSKANRIELFSLRSPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINI 60
           MSKANRIELFSLRSPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINI
Sbjct: 1   MSKANRIELFSLRSPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINI 60

Query: 61  AAVAVTILVRLIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVG 120
           AAVAVTILVRLIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVG
Sbjct: 61  AAVAVTILVRLIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVG 120

Query: 121 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWR 180
           ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWR
Sbjct: 121 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWR 180

Query: 181 AALLVPGVLMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVW 240
           AALLVPGVLMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVW
Sbjct: 181 AALLVPGVLMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVW 240

Query: 241 MLFITYGACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDKV 300
           MLFITYGACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDKV
Sbjct: 241 MLFITYGACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDKV 300

Query: 301 ALAGGLNRRATLLFVMMLGEGLGLLWFAHAGSVVAAVIAMLCFGLFTHMACGATYALVPF 360
           ALAGGLNRRATLLFVMMLGEGLGLLWFAHAGSVVAAVIAMLCFGLFTHMACGATYALVPF
Sbjct: 301 ALAGGLNRRATLLFVMMLGEGLGLLWFAHAGSVVAAVIAMLCFGLFTHMACGATYALVPF 360

Query: 361 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGNTQQTLSMLGVLVTISALCAVAIRFTAS 420
           IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGNTQQTLSMLGVLVTISALCAVAIRFTAS
Sbjct: 361 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGNTQQTLSMLGVLVTISALCAVAIRFTAS 420

Query: 421 DKSGEAVLVDNKVAA 435
           DKSGEAVL DNKVAA
Sbjct: 421 DKSGEAVLADNKVAA 435


>gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter
           protein [Herbaspirillum seropedicae SmR1]
          Length = 421

 Score = 81.3 bits (199), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 76/278 (27%), Positives = 117/278 (42%), Gaps = 28/278 (10%)

Query: 25  MAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINIAAVAVTILVRLIIGPMCDRFGPRK 84
           +AF VCF  W     +   IK   HL   +   +    V    L+R+ +G   DR+G R 
Sbjct: 16  LAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRI 75

Query: 85  TYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVGASFVITQYHTSVMFAPNVVGTANA 144
            +  L+L+  +P+  +++A ++  FL   L +G  G SF +   + +  F     G A  
Sbjct: 76  VFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPYVARWFKKERQGLA-M 134

Query: 145 ATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWRAALLVPGV---LMLVMAALYWRFT 201
              G GN+G  V + + P+L+A       S GW      LVP V   LML  A ++W  +
Sbjct: 135 GVFGAGNSGAAVNKFIAPVLIA-------SAGW-----TLVPQVYAGLMLAAALIFWLGS 182

Query: 202 QDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVWMLFITYGACFGVEIFIHNIAA 261
              P             + S  R  +        + RVW     Y   FG  + +     
Sbjct: 183 ATDPSHQ----------VSSDTR--FMDQVRMLRDPRVWRYCQYYSVVFGGYVGLSLWMV 230

Query: 262 IYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDK 299
            YYV  +   +  A + AA F L     RALGG++SD+
Sbjct: 231 KYYVTEYGFDIRHAALLAACFSLPGGVLRALGGWLSDR 268


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.329    0.141    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 997,591
Number of extensions: 33307
Number of successful extensions: 261
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 267
Number of HSP's successfully gapped: 5
Length of query: 435
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 347
Effective length of database: 1,203,190
Effective search space: 417506930
Effective search space used: 417506930
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 132 (55.5 bits)