BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0081.p (379 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 733 0.0 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 262 3e-71 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 258 6e-70 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 214 7e-57 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 151 7e-38 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 733 bits (1892), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/372 (98%), Positives = 371/372 (99%) Query: 8 LCAYAFAHAALAQSPAAASAASSSSVTVYGVIDMSLVYTSKVGPDNGSRASLDSGDAMAS 67 +CAYAFAHAALAQSPAAASAASSSSVTVYGVIDMSLVYTSKVGPDNGSRASLDSGDAMAS Sbjct: 1 MCAYAFAHAALAQSPAAASAASSSSVTVYGVIDMSLVYTSKVGPDNGSRASLDSGDAMAS 60 Query: 68 RIGLRGREELGDGLAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAGRFGTLTVGRQTD 127 RIGLRGREELGDGLAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAGRFGTLTVGRQTD Sbjct: 61 RIGLRGREELGDGLAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAGRFGTLTVGRQTD 120 Query: 128 YLEEIGQQYTSFQIFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGE 187 YLEEIGQQYTSFQIFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGE Sbjct: 121 YLEEIGQQYTSFQIFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGE 180 Query: 188 VAGSTRSGQSLGVGGNFRRGDVGVGAAYFQSKLASDALPARAGDTELKTFTLGASYQAGP 247 VAGSTRSGQSLGVGGNFRRG+VGVGAAYFQSKLASDALPARAGDTELKTFTLGASYQAGP Sbjct: 181 VAGSTRSGQSLGVGGNFRRGNVGVGAAYFQSKLASDALPARAGDTELKTFTLGASYQAGP 240 Query: 248 AKLFAAWSQIRRPTQQPLSSSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADL 307 AKLFAAWSQIRRPTQQPL+SSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADL Sbjct: 241 AKLFAAWSQIRRPTQQPLASSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADL 300 Query: 308 SRAAGGATRGSTTQFNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSS 367 SRAAGGATRGSTTQ NLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSS Sbjct: 301 SRAAGGATRGSTTQVNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSS 360 Query: 368 QNVLRVGLRHKF 379 QNVLRVGLRHKF Sbjct: 361 QNVLRVGLRHKF 372 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 262 bits (670), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 161/382 (42%), Positives = 223/382 (58%), Gaps = 46/382 (12%) Query: 31 SSVTVYGVIDMSLVYTSKVGPDNG---SRASLDSGDAMASRIGLRGREELGDGLAAIFQL 87 SSVT+YG++D +VY SKV G S+ SL+SG SRIG +G E+LG GL+A+F L Sbjct: 22 SSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNL 81 Query: 88 ETGFAGDDGAM---ATPGTLFDRKSVVGLAGRFGTLTVGRQTDYLEEIGQQYTSFQIFGG 144 E GF+ D GA+ T TLF RKSVVGLAG FG++ +GRQTD+ + I YTS Q FGG Sbjct: 82 EAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLGRQTDFADTI-SSYTSVQDFGG 140 Query: 145 GGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGSTRSGQSLGVGGNF 204 A H +LDR+ G R+NNS+ + TA G G+ YG GE AG T +GQ+ GVGG + Sbjct: 141 WTGAAGH--NLDRLEGTRTNNSISYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKY 198 Query: 205 RRGDVGVGAAYFQSKLASDALPARA-----------------GDTELKTFTLGASYQAGP 247 G +G+G Y+QSK S PA A G T K + ASYQ GP Sbjct: 199 DNGPLGLGINYYQSKAGS--TPADASLLAATGTNQFTNGTNNGSTAQKVLNVAASYQFGP 256 Query: 248 AKLFAAWSQIRRP--TQQPLSSSG---LVAITSAT-----RANVLDVGVDYALDSKLHLL 297 A+++ WS++++ TQ L+++G L I++ T +A++ ++G Y+L L LL Sbjct: 257 ARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIGTAYSLSPSLKLL 316 Query: 298 ASVIHDRADLSRAAGGATRGSTTQFNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAA 357 A+V H RA +G + TQ +LG D LSKRTD+YA + +A D+ NPG+ Sbjct: 317 AAVDHTRAQFDGVSG---KAKLTQVSLGTDYWLSKRTDLYAFLAYSKATDLNNPGV---- 369 Query: 358 YSSAPADDSSQNVLRVGLRHKF 379 + + D +Q + VG+RHKF Sbjct: 370 -ADSSGTDGNQGAVAVGIRHKF 390 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 258 bits (659), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 160/380 (42%), Positives = 224/380 (58%), Gaps = 45/380 (11%) Query: 31 SSVTVYGVIDMSLVYTSKVGPDN-------GSRASLDSGDAMASRIGLRGREELGDGLAA 83 SSVT+YG++D + Y+SKVG + GS+ ++SG SRIG +G E+LG GL+A Sbjct: 22 SSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSGVIQGSRIGFKGVEDLGGGLSA 81 Query: 84 IFQLETGFAGDDGAM-----ATPGTLFDRKSVVGLAGRFGTLTVGRQTDYLEEIGQQYTS 138 +F LETGF DDG + T LF RKSVVGL+G FGT+ +GRQTD+ + I YT+ Sbjct: 82 VFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGFGTVLLGRQTDFADTI-SAYTA 140 Query: 139 FQIFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGSTRSGQSL 198 FGG V A +SL+R+ G R+NNS+ + TA G G+ YG GE AG T +GQ+ Sbjct: 141 VADFGG--VVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGNLIYGFGEQAGKTSAGQAY 198 Query: 199 GVGGNFRRGDVGVGAAYFQSKLASDA-----LPAR-----------AGDTELKTFTLGAS 242 G+GG + G +G+G Y+QSK S +PA AG+T LK+ + AS Sbjct: 199 GIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANTALGVAGNTALKSLNVVAS 258 Query: 243 YQAGPAKLFAAWSQIRRPTQQPLSSSGLVA---ITSATRANVLDVGVDYALDSKLHLLAS 299 YQ GPA+++A +S+++ Q L + VA + +A +A+ ++G Y+L L LLA+ Sbjct: 259 YQFGPARVYANYSRVK----QDLKGTPTVATATLANAQKADTYEIGTAYSLSPSLKLLAA 314 Query: 300 VIHDRADLSRAAGGATRGSTTQFNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYS 359 V H RA + G T+G TQ +LG D LSKRTD+YA S +A D+ NPG+ A Sbjct: 315 VDHSRATFN---GVNTKGKLTQISLGADYFLSKRTDLYAFVSNMRASDIQNPGVTGGATG 371 Query: 360 SAPADDSSQNVLRVGLRHKF 379 S D+SQ + G+RHKF Sbjct: 372 S----DASQTAIITGIRHKF 387 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 214 bits (546), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 157/425 (36%), Positives = 215/425 (50%), Gaps = 66/425 (15%) Query: 1 MKKISLSLCAY-AFAHAALAQSPAAASAASSSSVTVYGVIDMSLVYTSKV---GPDNGSR 56 MKK L+L AFA AA AQS SS+T+YG ID SL YT+K+ G + GS Sbjct: 1 MKKSLLALAVLGAFAGAAHAQS---------SSITIYGAIDASLTYTNKIATDGKNTGSL 51 Query: 57 ASLDSGDAMASRIGLRGREELGDGLAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAGR 116 +++SG SR+G++G E+LG GL A+F LE GF D G ++ R++ VGL+G Sbjct: 52 TAVESGLVKGSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGLSGN 111 Query: 117 FGTLTVGRQTDYLEEIGQQYTSFQIFGGGGVRA-AHFNSLDRITGARSNNSLRFDTAEYG 175 FGT+ VGRQ D L +IG ++ GG GV H LDR G R NNS+R+++ + Sbjct: 112 FGTVLVGRQNDPLNDIGGIASA----GGFGVPGTVHSKGLDRTDGQRLNNSIRYNSPNFN 167 Query: 176 GLRGSFYYGAGEVAGSTRSGQSLGVGGNFRRGDVGVGAAYFQSKLA-----SDALPARA- 229 GL S YG GE G G+S+G+G + G + +G YFQSK+ S + A+A Sbjct: 168 GLSFSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAA 227 Query: 230 ----------GDTELKTFTLGASYQAGPAKLFAAWSQIRRP------------------- 260 GDT LKT+TL A+YQ GPAK++ A+S +R P Sbjct: 228 CNGAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMYD 287 Query: 261 ----TQQPLSSSGLVAITSATR--ANVLDVGVDYALDSKLHLLASVIHDRADLSRAAGGA 314 T S + +I A + D+G+ Y L L L+++V H RA GA Sbjct: 288 AKTGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFV----GA 343 Query: 315 TRGSTTQFNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSSQNVLRVG 374 G Q NLG LSKRT YA+ ++ D+ N G+ + P D SQ + G Sbjct: 344 ASGRLLQLNLGAKYDLSKRTSAYALVRNLRSSDMYNVGL---SKDQEPGIDRSQTAISTG 400 Query: 375 LRHKF 379 L H F Sbjct: 401 LIHTF 405 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 151 bits (382), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 124/373 (33%), Positives = 182/373 (48%), Gaps = 52/373 (13%) Query: 24 AASAASSSSVTVYGVIDMSLVYTSKVGPDNGSRASL---DSGDAMASRIGLRGREELGDG 80 A +A + SSV++YG+ID+ L Y S G A L DSG A SRIG +G E+LG G Sbjct: 15 ATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQGSRIGFKGTEDLGGG 74 Query: 81 LAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAG-RFGTLTVGRQTDYLEEIGQQYTSF 139 + A F LETGF DDG++ G LF R+S VGL ++G+L+VGRQ D++ IG QY Sbjct: 75 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG 134 Query: 140 QI-----FGGG-GVRAAHFNSLDR--ITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGS 191 + FG G AAH +L+ G R+NNS+++++A++GG G GE AG+ Sbjct: 135 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN 194 Query: 192 TRSGQSLGVGGNFRRGDVGVGAAYFQSKLASDALPARAGDTELKTFTLGASYQAGPAKLF 251 +G+++ G + G F LA + G + + GA YQ G F Sbjct: 195 NNAGRTVSATGQYDNGP-------FSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPF 247 Query: 252 AAWSQIRRPTQQPLSSSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADLSRAA 311 +Q++ ++ +A ++G YAL L A+ + + + Sbjct: 248 GVVTQVKN-------------TNTSAKATTYELGTVYALTPLWDLAAAYQFQKRN--QGV 292 Query: 312 GGATRGSTTQFNLGVDLLLSKRTDVY--AMASRQQ---ARDVLNPGIISAAYSSAPADDS 366 G A T VD +SKRTD+Y A+ R + A V GI SA Sbjct: 293 GNAQALVAT-----VDYKISKRTDLYVGAVYDRDKGYNAYPVFGGGIQSAT--------G 339 Query: 367 SQNVLRVGLRHKF 379 +Q LR+G+RH+F Sbjct: 340 NQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,061,315 Number of extensions: 44666 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 14 Length of query: 379 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 292 Effective length of database: 1,207,925 Effective search space: 352714100 Effective search space used: 352714100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)