BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0076.p
         (221 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra...   442   e-126
gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm...   110   9e-26
gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ...    86   3e-18

>gi|300312188|ref|YP_003776280.1| hemolysin secretion-related
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 447

 Score =  442 bits (1137), Expect = e-126,   Method: Compositional matrix adjust.
 Identities = 219/221 (99%), Positives = 219/221 (99%)

Query: 1   VADHTVSRHAWLEKEQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKIIEASQQD 60
           VADHTVSRHAWLEKEQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADK IEASQQD
Sbjct: 227 VADHTVSRHAWLEKEQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQD 286

Query: 61  QRRADEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDV 120
           QRRADEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDV
Sbjct: 287 QRRADEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDV 346

Query: 121 GFVEIGQVAEVKIDAFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDG 180
           GFVEIGQ AEVKIDAFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDG
Sbjct: 347 GFVEIGQAAEVKIDAFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDG 406

Query: 181 RKVQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHER 221
           RKVQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHER
Sbjct: 407 RKVQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHER 447


>gi|300312349|ref|YP_003776441.1| hemolysin-type secretion
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score =  110 bits (275), Expect = 9e-26,   Method: Compositional matrix adjust.
 Identities = 71/223 (31%), Positives = 123/223 (55%), Gaps = 18/223 (8%)

Query: 6   VSRHAWLEKEQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKIIEASQQDQRRAD 65
           V R   L  ++   D++ Q+++ RN          K   ++ ++  K+ E     + + +
Sbjct: 175 VGRADVLRLQRNVADIRAQINNKRN----------KYFQEALVDMTKVQEDLNTQREQLE 224

Query: 66  EHSKLLK---LVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGF 122
           + +++L+   L AP  G V+ + V TIGGVV +   +M+I+P D+ I VEA +   D  +
Sbjct: 225 DRTQVLEHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAY 283

Query: 123 VEIGQVAEVKIDAFDYTKYGTLPARVVHISRDAIQDE-KKGLLYSARIVLDQSSLIVDGR 181
           V++GQ A VK+DA+DY+ YGTL   VV++S D + DE ++G +   R+ +     +  GR
Sbjct: 284 VKVGQAANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGR 343

Query: 182 K---VQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHER 221
           K   +Q+  GM A VEIK   R V+ +   P+ +  +E++ ER
Sbjct: 344 KADAIQVKPGMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386


>gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD
           family secretion protein [Herbaspirillum seropedicae
           SmR1]
          Length = 457

 Score = 85.5 bits (210), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 57/203 (28%), Positives = 103/203 (50%), Gaps = 4/203 (1%)

Query: 21  LQGQLDDARNQRATLVAQTRKDAHDSRIEADKIIEASQQDQRRADEHSKLLKLVAPVTGT 80
           L   + +A+ ++    ++ R DA      A+  +   Q++ +  ++     ++ AP+ G 
Sbjct: 254 LNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDRVSRSEVKAPMDGV 313

Query: 81  VQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQVAEVKIDAFDYTK 140
           V ++ ++TIGGVV    P++++ P D ++ +EA +   D   +  G  A VK+ A+DY  
Sbjct: 314 VNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGARARVKVSAYDYGV 373

Query: 141 YGTLPARVVHISRDAIQDE---KKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVEIKT 197
           YG +   V  IS D + DE   +    Y  +I + +    +  +K+ L  GM A+ +I  
Sbjct: 374 YGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQ-QKLALMPGMTASADIIV 432

Query: 198 GNRRVIEYALSPLIRHQKEALHE 220
           G R V +Y +SPL R  + AL E
Sbjct: 433 GRRTVWQYLMSPLSRFGQGALRE 455


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.131    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 636,521
Number of extensions: 23606
Number of successful extensions: 107
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 104
Number of HSP's successfully gapped: 3
Length of query: 221
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 139
Effective length of database: 1,231,600
Effective search space: 171192400
Effective search space used: 171192400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 129 (54.3 bits)