BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0072.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312186|ref|YP_003776278.1| calcium binding secreted hemoly... 832 0.0 gi|300312185|ref|YP_003776277.1| calcium binding secreted hemoly... 370 e-103 gi|300312190|ref|YP_003776282.1| hemolysin-type calcium binding ... 323 3e-89 gi|300311899|ref|YP_003775991.1| hemolysin-type calcium binding ... 223 4e-59 >gi|300312186|ref|YP_003776278.1| calcium binding secreted hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 1080 Score = 832 bits (2149), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/412 (99%), Positives = 411/412 (99%) Query: 25 ILDHNGKAMSAAQVAALDLNHNGKLEAGELADLKAWTDSNEDDLAQASELTSLSDALKAA 84 ILDHNGKAMSAAQVAALDLNHNGKLEAGELADLKAWTDSNEDDLAQASELTSLSDALKAA Sbjct: 430 ILDHNGKAMSAAQVAALDLNHNGKLEAGELADLKAWTDSNEDDLAQASELTSLSDALKAA 489 Query: 85 GLPNTLRATDYSIYTVGKGPRQEWNFKSLLATLSGQGAFFFVLPATPQAGTAGDDQLLGS 144 GLPNTLRATDYSIYTVGKGPRQEWNFKSLLATLSGQGAFFFVLPATPQAGTAGDDQLLGS Sbjct: 490 GLPNTLRATDYSIYTVGKGPRQEWNFKSLLATLSGQGAFFFVLPATPQAGTAGDDQLLGS 549 Query: 145 TGEDSLHGGDGKDWLFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRVVETINGGHD 204 TGEDSLHGGDGKDWLFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRVVETINGGHD Sbjct: 550 TGEDSLHGGDGKDWLFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRVVETINGGHD 609 Query: 205 TVIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGN 264 TVIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGN Sbjct: 610 TVIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGN 669 Query: 265 DSYFVDDAGDQVSEAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYG 324 DSYFVDDAGDQV+EAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYG Sbjct: 670 DSYFVDDAGDQVTEAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYG 729 Query: 325 APKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSA 384 APKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSA Sbjct: 730 APKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSA 789 Query: 385 SAKGGSSALDQKRLLESYGLRAEYTLRYDQDKLAALVKEGRGVMVSVDIRRI 436 SAKGGSSALDQKRLLESYGLRAEYTL+YDQDKLAALVKEGRGVMVSVD RR+ Sbjct: 790 SAKGGSSALDQKRLLESYGLRAEYTLKYDQDKLAALVKEGRGVMVSVDSRRL 841 >gi|300312185|ref|YP_003776277.1| calcium binding secreted hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 984 Score = 370 bits (951), Expect = e-103, Method: Compositional matrix adjust. Identities = 220/439 (50%), Positives = 275/439 (62%), Gaps = 33/439 (7%) Query: 25 ILDHNGKAMSAAQVAALDLNHNGKLEAGELADLKAWTDSNEDDLAQASELTSLSDALKAA 84 LD K ++ AQ+AALD N + L++ ELA L AWTD NED +AQA ELTSL A+ AA Sbjct: 307 FLDGADKPLTEAQLAALDKNKDAHLDSDELAGLHAWTDLNEDGIAQAGELTSLPSAMTAA 366 Query: 85 GLPNTLRATDYSIYTVG----KGPRQEWNFKSLLATLSGQ----GAFFFVLPATPQA--- 133 GL + LR DY +Y G + P Q +S A SG+ GA VL AT Sbjct: 367 GLASGLRVADYQVYLRGNASFRTPEQVQAMRSA-AQASGKKNQGGAGNDVLFATNGEEIN 425 Query: 134 GTAGDDQLLGSTGEDSL------------------HGGDGKDWLFGFDGNDVLDGGLGAD 175 G GDD + G G+D+L +GG GKD L G GND+LDGG G D Sbjct: 426 GNGGDDVIYGGDGDDTLVGGTYSALARGETDNDVLYGGAGKDSLSGGVGNDLLDGGTGDD 485 Query: 176 TLYGGDGDDTYIVNSSDDRVVETINGGHDTVIASCSTILAANVEDLRLVEGGNLNGAGNT 235 +YGG GDD Y+V+S+ D V E + G DTV+A ST L NVEDLRL+EGG+ NG GN Sbjct: 486 RMYGGAGDDIYVVDSAGDGVFENADAGRDTVLAGMSTTLGENVEDLRLLEGGSYNGTGNF 545 Query: 236 LDNLIVGNGQDNILDGGVGADTMIGGLGNDSYFVDDAGDQVSEAADEGVDTVYSRISMRL 295 LDN + GN QDNILDG GADTM+GG GND YFVD+ GDQV E A EG+DTVYS +SM L Sbjct: 546 LDNRLTGNSQDNILDGLAGADTMMGGQGNDRYFVDNVGDQVIEEAGEGIDTVYSTVSMTL 605 Query: 296 AANVENLTLLDGGTPLRGTVEGKAALIYGAPKAYDLDYQQGNGVTGYDGTCGETSVVNVL 355 ANVENLTL+D TP+ G + G+ ALIYG PK+Y +DY+QG+ G+ GTCG TSV N+L Sbjct: 606 GANVENLTLMDARTPVHGEIYGQDALIYGTPKSYLMDYRQGDARPGFLGTCGVTSVANML 665 Query: 356 RMADQWVSEKEVVQRAIDKKWCITDAPS--ASAKGGSSALDQKRLLESYGLRAEYTLRYD 413 MA V+EKE +QRAID+ WC +D PS + +GG + D L+ S+G+ E RYD Sbjct: 666 TMAGVNVTEKETLQRAIDRDWCDSD-PSLPITLRGGVTCNDLAELMGSFGVAGEVDYRYD 724 Query: 414 QDKLAALVKEGRGVMVSVD 432 + +L L+K G V + D Sbjct: 725 EARLVKLLKGGHVVTLCAD 743 >gi|300312190|ref|YP_003776282.1| hemolysin-type calcium binding protein [Herbaspirillum seropedicae SmR1] Length = 1139 Score = 323 bits (827), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 172/306 (56%), Positives = 209/306 (68%), Gaps = 2/306 (0%) Query: 146 GEDSLHGGDGKDWLFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRVVETINGGHDT 205 G+D L+GG G D LFG GNDVLDGG G D+L GG GDDTY+V+ + D + E + GHDT Sbjct: 610 GDDLLYGGAGNDSLFGGSGNDVLDGGSGNDSLVGGLGDDTYVVDGAGDTIREDADQGHDT 669 Query: 206 VIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGND 265 V+ASCS L N+EDLRL EGG NG GN DNLI GN QDNILDGG GADT++GG GND Sbjct: 670 VVASCSYTLGRNLEDLRLAEGGQFNGTGNAQDNLITGNSQDNILDGGRGADTLMGGTGND 729 Query: 266 SYFVDDAGDQVSEAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYGA 325 SYFVDD D+V E A EGVDTV+SRIS L AN+ENLTLLD TP V G+ L+YG Sbjct: 730 SYFVDDVNDKVVEQAGEGVDTVFSRISTTLGANLENLTLLDSATPQHEIVNGRDMLVYGY 789 Query: 326 PKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSAS 385 P +LDY QG+ V GY GTCGETSVVN+ MA V+E + V+RAI WC+T S Sbjct: 790 PHYAELDYSQGDAVAGYTGTCGETSVVNLCLMAGLAVTEAQAVKRAIANGWCVTSG--ES 847 Query: 386 AKGGSSALDQKRLLESYGLRAEYTLRYDQDKLAALVKEGRGVMVSVDIRRIDDRFISIQT 445 GGS+ DQ LL SYG+RA+ YD+ L ++KEGRG +VSV+ ++ D ++Q Sbjct: 848 QLGGSNQYDQVALLASYGIRAKAGYGYDEKALVKVLKEGRGALVSVNAGKLWDNPGAVQL 907 Query: 446 GSRTEV 451 G+ V Sbjct: 908 GNGNHV 913 Score = 86.7 bits (213), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 94/282 (33%), Positives = 126/282 (44%), Gaps = 80/282 (28%) Query: 134 GTAGDDQLLGSTGEDSLHGGDGKDWLFGFDGN---------DVLD---------GGLGAD 175 GT GDD L G G D+L G +G D LFG DG+ D+LD GGLG D Sbjct: 438 GTTGDDTLWGGAGNDTLFGREGNDRLFGEDGDDQLLGGEGFDLLDGGAGDDQLFGGLGND 497 Query: 176 TLYGGDGDDTYI------------VNSSDDRVVETING--------GHDTV-IASCSTIL 214 ++GGDGDDT + +DD ++ G G+D + L Sbjct: 498 QMWGGDGDDTMMGSDVSNTGRPLPAGQTDDDIMFGGAGKDGMFGGAGNDQMWGGDGDDGL 557 Query: 215 AANVEDLRLV-EGGN---LNGAGNTL------DNLIVGN------------GQDNIL--- 249 N D +L E GN G GN + D+LI G+ G D++L Sbjct: 558 YGNEGDDKLYGEAGNDHIYGGKGNDVIQGGDGDDLIYGDDMEYFWMQPDTTGGDDLLYGG 617 Query: 250 ---------------DGGVGADTMIGGLGNDSYFVDDAGDQVSEAADEGVDTVYSRISMR 294 DGG G D+++GGLG+D+Y VD AGD + E AD+G DTV + S Sbjct: 618 AGNDSLFGGSGNDVLDGGSGNDSLVGGLGDDTYVVDGAGDTIREDADQGHDTVVASCSYT 677 Query: 295 LAANVENLTLLDGGTPLRGTVEGKAALIYGAPKAYDLDYQQG 336 L N+E+L L +GG GT + LI G + LD +G Sbjct: 678 LGRNLEDLRLAEGGQ-FNGTGNAQDNLITGNSQDNILDGGRG 718 >gi|300311899|ref|YP_003775991.1| hemolysin-type calcium binding protein [Herbaspirillum seropedicae SmR1] Length = 1974 Score = 223 bits (567), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 119/226 (52%), Positives = 148/226 (65%), Gaps = 1/226 (0%) Query: 206 VIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGND 265 V A + IL DL V +LNG GN L N I GN +DNILDG GADT+ GG GND Sbjct: 672 VTARKTLILDDTTNDLD-VGADHLNGIGNALANRITGNDRDNILDGMAGADTLTGGKGND 730 Query: 266 SYFVDDAGDQVSEAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYGA 325 SY+VDDAGD+V E A EG+DTVY+ +S LAANVENL LLD P V G L+YG Sbjct: 731 SYYVDDAGDKVIEKAGEGLDTVYATVSTTLAANVENLVLLDASKPQTAVVNGIHVLVYGR 790 Query: 326 PKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSAS 385 PK+Y LDY+QG+ V GY GTCGET+V N+ + D+ SEKEVV+RAI +K C T P Sbjct: 791 PKSYQLDYKQGDAVKGYRGTCGETAVANITMLGDRPASEKEVVERAIKEKLCDTLTPYDQ 850 Query: 386 AKGGSSALDQKRLLESYGLRAEYTLRYDQDKLAALVKEGRGVMVSV 431 +GGS Q+ LL+ +GLR+ T +D+ +A +KEG+GV V V Sbjct: 851 FRGGSDEKQQQALLKDFGLRSSVTEGFDEKSVAQSIKEGKGVAVGV 896 Score = 213 bits (543), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 118/233 (50%), Positives = 149/233 (63%), Gaps = 2/233 (0%) Query: 206 VIASCSTILAANVEDLRLVEGGNLNGAGNTLDNLIVGNGQDNILDGGVGADTMIGGLGND 265 V A + L + +DL L G +LNG GN LDN + GN Q+NILDG G DTMIGG G+D Sbjct: 1506 VAAPKNLSLDEDTDDLSL--GSSLNGIGNALDNRLTGNDQNNILDGMGGTDTMIGGKGDD 1563 Query: 266 SYFVDDAGDQVSEAADEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYGA 325 SY++D+AGD++ E A EGVDTVY+ S LAANVENL LLD P V G L+YG Sbjct: 1564 SYYLDNAGDKIVEQAGEGVDTVYATASTTLAANVENLVLLDASKPQSAVVNGVNVLVYGM 1623 Query: 326 PKAYDLDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSAS 385 P++Y LDY QG V GY GTCGETSV NV M VSEKEVVQRAI + C T A SA Sbjct: 1624 PRSYQLDYDQGGEVEGYWGTCGETSVANVTLMGGHAVSEKEVVQRAIKENLCDTAAESAD 1683 Query: 386 AKGGSSALDQKRLLESYGLRAEYTLRYDQDKLAALVKEGRGVMVSVDIRRIDD 438 +G +S D++ LL+ +G A D +A +K+G+GV++SV ++ D Sbjct: 1684 TRGATSEDDRQALLQDFGFAASVEEELDLKAVAQSIKDGKGVIISVSASKLWD 1736 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 55/123 (44%), Positives = 82/123 (66%), Gaps = 1/123 (0%) Query: 315 VEGKAALIYGAPKAYD-LDYQQGNGVTGYDGTCGETSVVNVLRMADQWVSEKEVVQRAID 373 ++GK L+YG PK+YD L+Y QG V+GY GTC E S+ N+L +A Q VSE +V++RAI Sbjct: 191 IDGKDVLVYGYPKSYDMLNYSQGFAVSGYYGTCTEASIANLLSIAGQDVSEADVIKRAIV 250 Query: 374 KKWCITDAPSASAKGGSSALDQKRLLESYGLRAEYTLRYDQDKLAALVKEGRGVMVSVDI 433 WC T + + +GG+ Q+ LL+SYG+ +D +KLA LVK+GRGV++ V+ Sbjct: 251 NGWCDTTSDNPCLRGGAMTGQQRDLLKSYGVETGVLNDFDPEKLAMLVKQGRGVILGVES 310 Query: 434 RRI 436 + + Sbjct: 311 KEL 313 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 51/153 (33%), Positives = 82/153 (53%), Gaps = 2/153 (1%) Query: 281 DEGVDTVYSRISMRLAANVENLTLLDGGTPLRGTVEGKAALIYGAPKAY-DLDYQQGNGV 339 D G + +Y+ ++ A V + D P G G +IYG PKAY +L+Y Q + Sbjct: 378 DYGCNAIYTLDPIK-KAKVPDTGPADPSKPQMGKEYGVKLIIYGDPKAYHNLNYDQPVVM 436 Query: 340 TGYDGTCGETSVVNVLRMADQWVSEKEVVQRAIDKKWCITDAPSASAKGGSSALDQKRLL 399 Y T GE +V N+ +A Q +S K V+Q AID+K C T GG+++ +Q LL Sbjct: 437 AMYTETSGEIAVANIAALAGQSLSPKTVLQTAIDEKLCDTTKKDRDLGGGTTSENQVALL 496 Query: 400 ESYGLRAEYTLRYDQDKLAALVKEGRGVMVSVD 432 + +G+ A +D + +A + EG+GV++ V+ Sbjct: 497 KKFGIAATLEQGFDANAVAQHIIEGKGVILRVN 529 Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 50/116 (43%), Positives = 70/116 (60%), Gaps = 7/116 (6%) Query: 138 DDQLLGSTGEDSLHG-GDGKD-WLFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRV 195 DD LGS SL+G G+ D L G D N++LDG G DT+ GG GDD+Y ++++ D++ Sbjct: 1519 DDLSLGS----SLNGIGNALDNRLTGNDQNNILDGMGGTDTMIGGKGDDSYYLDNAGDKI 1574 Query: 196 VETINGGHDTVIASCSTILAANVEDLRLVEGGNLNGA-GNTLDNLIVGNGQDNILD 250 VE G DTV A+ ST LAANVE+L L++ A N ++ L+ G + LD Sbjct: 1575 VEQAGEGVDTVYATASTTLAANVENLVLLDASKPQSAVVNGVNVLVYGMPRSYQLD 1630 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 40/93 (43%), Positives = 57/93 (61%), Gaps = 1/93 (1%) Query: 159 LFGFDGNDVLDGGLGADTLYGGDGDDTYIVNSSDDRVVETINGGHDTVIASCSTILAANV 218 + G D +++LDG GADTL GG G+D+Y V+ + D+V+E G DTV A+ ST LAANV Sbjct: 705 ITGNDRDNILDGMAGADTLTGGKGNDSYYVDDAGDKVIEKAGEGLDTVYATVSTTLAANV 764 Query: 219 EDLRLVEGGNLNGA-GNTLDNLIVGNGQDNILD 250 E+L L++ A N + L+ G + LD Sbjct: 765 ENLVLLDASKPQTAVVNGIHVLVYGRPKSYQLD 797 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,485,056 Number of extensions: 63871 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 14 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)