BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0068.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 522 e-149 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 107 1e-24 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 98 6e-22 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 94 8e-21 gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 94 1e-20 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 522 bits (1344), Expect = e-149, Method: Compositional matrix adjust. Identities = 260/262 (99%), Positives = 261/262 (99%) Query: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSRDDWIAAVDANMLTPI 120 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSR+DWIAAVDANMLTPI Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 121 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT Sbjct: 121 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 Query: 181 INNLLPGPFETDRLRATARGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCS 240 INNLLPGPFETDRLRATA GAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCS Sbjct: 181 INNLLPGPFETDRLRATASGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCS 240 Query: 241 AQAGFITGQNLLMDGGAYPGTF 262 AQAGFITGQNLLMDGGAYPGTF Sbjct: 241 AQAGFITGQNLLMDGGAYPGTF 262 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 61/177 (34%), Positives = 92/177 (51%), Gaps = 15/177 (8%) Query: 86 DILVTNAGGPKPGDFRDWSRDDWIAAVDANMLTPIELIKATVDGMIARRFGRIVNITSSA 145 D+LV NAG + G FR S+DDW A +D N+ + + K ++GM R FGRI+NI+S Sbjct: 83 DVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEGMCERGFGRIINISS-- 140 Query: 146 VRAPIDILGLSN--GARSGLTGFVAGLARKTVAHNVTINNLLPGPFETDRLRATARGAAQ 203 V G +N A++G+ GF LA++ VT+N + PG TD +RA Sbjct: 141 VNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGTDMVRAIR----- 195 Query: 204 ESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCSAQAGFITGQNLLMDGGAYPG 260 E+L + P R G+P E A++ S++ G+ TG + ++GG Y G Sbjct: 196 ------PEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGLYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 98.2 bits (243), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 89/268 (33%), Positives = 127/268 (47%), Gaps = 34/268 (12%) Query: 5 LNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEV---AATGAI 61 L G+ ALV +S+G+GRA A LAR+G V A E+ AA E A + A GA+ Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTAT-SESGAAAITEYLAALGPEAGRGAV 64 Query: 62 ITTVSCDITTPEGRALALAACPAPD-----ILVTNAGGPKPGDFRDWSRDDWIAAVDANM 116 + + + +AL D ILV NAG + ++W + + N+ Sbjct: 65 LNVNDAEAS------VALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNL 118 Query: 117 LTPIELIKATVDGMIARRFGRIVNITSSAVRA--PIDILGLSN--GARSGLTGFVAGLAR 172 L +A + GM+ R GRI+NITS A P G N A++G+ G LAR Sbjct: 119 TAVGRLSRAVLRGMMKARTGRIINITSVVGEAGNP----GQMNYAAAKAGVAGMSRALAR 174 Query: 173 KTVAHNVTINNLLPGPFETDRLRATARGAAQESGRSVDEILAERRKLNPAGRFGDPAEFG 232 + + N+T+N + PG +TD RA Q+S + +I P GR G E Sbjct: 175 EIGSRNITVNCVAPGFIDTDMTRALNE---QQSAAILQQI--------PLGRLGAAEEIA 223 Query: 233 DACAYLCSAQAGFITGQNLLMDGGAYPG 260 A A+L S QA +ITG L ++GG Y G Sbjct: 224 YAVAFLASPQAAYITGTTLNVNGGMYMG 251 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 94.4 bits (233), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 73/258 (28%), Positives = 116/258 (44%), Gaps = 18/258 (6%) Query: 4 RLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGAIIT 63 RL GKT L+ A+++G+GRA AREG V + L+ E+A + T Sbjct: 7 RLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLD--------ELAGIAGVET 58 Query: 64 TVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSRDDWIAAVDANMLTPIELI 123 + D+T +A D+L AG G+ + W + + N I Sbjct: 59 HL-LDVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTI 117 Query: 124 KATVDGMIARRFGRIVNITSSAVRAPIDILGLSN-----GARSGLTGFVAGLARKTVAHN 178 +A + GM+A++ G IVNI S+A + G++N +++ + G +A VA Sbjct: 118 RAVLPGMLAKKAGSIVNIASAAS----SVKGVANRFAYGASKAAVVGLTKSVAADFVAQG 173 Query: 179 VTINNLLPGPFETDRLRATARGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYL 238 + N + PG E+ L A+E+G+S +E+ A P GR G E YL Sbjct: 174 IRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYL 233 Query: 239 CSAQAGFITGQNLLMDGG 256 S ++ F TG ++DGG Sbjct: 234 ASDESNFTTGSIHMIDGG 251 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 79/269 (29%), Positives = 125/269 (46%), Gaps = 13/269 (4%) Query: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 M+L+L GK A+V S G+GRA A +LAREGV V ++AR + LE A + +TG Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALS---QSTGT 57 Query: 61 IITTVSCDITTPEGRALALAACPAP----DILVTNA---GGPKPGDFRDWSRDDWIAAVD 113 + V+ D++ E A+A DILV A GG + + S + + ++ Sbjct: 58 EVIAVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDIN 117 Query: 114 ANMLTPIELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARK 173 ++ + KA M A +GRI+NI R + G+ N A LT L+ + Sbjct: 118 IKVVGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLT---KTLSDQ 174 Query: 174 TVAHNVTINNLLPGPFETDRLRATARGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGD 233 +T+N + PG ET + A+ G + +++ K P R E D Sbjct: 175 LGPDGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIAD 234 Query: 234 ACAYLCSAQAGFITGQNLLMDGGAYPGTF 262 +L S +A ITG+++ +DGG G + Sbjct: 235 VVLFLASPRAAAITGESIAVDGGITRGIY 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 842,342 Number of extensions: 35040 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 33 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)