BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0065.p (321 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312111|ref|YP_003776203.1| LysR family transcription regul... 657 0.0 gi|300312305|ref|YP_003776397.1| LysR family transcription regul... 239 3e-64 gi|300311542|ref|YP_003775634.1| LysR family transcription regul... 93 4e-20 gi|300309393|ref|YP_003773485.1| LysR family transcription regul... 89 5e-19 gi|300311724|ref|YP_003775816.1| LysR family transcription regul... 85 7e-18 >gi|300312111|ref|YP_003776203.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 321 Score = 657 bits (1694), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/321 (99%), Positives = 321/321 (100%) Query: 1 MLFDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLT 60 MLFDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLT Sbjct: 1 MLFDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLT 60 Query: 61 PAGHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHP 120 PAGHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHP Sbjct: 61 PAGHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHP 120 Query: 121 QVNVALEERLNHDILRGIQEGTADIGIVAGPVQGEGLQILNFSTDRLVLATALEHPLADA 180 QVNVALEERLNHDILRGIQEGTADIGIVAGPVQGEGLQILNFSTDRLVLATALEHPLADA Sbjct: 121 QVNVALEERLNHDILRGIQEGTADIGIVAGPVQGEGLQILNFSTDRLVLATALEHPLADA 180 Query: 181 PSLPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGIG 240 PSLPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGIG Sbjct: 181 PSLPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGIG 240 Query: 241 ILPRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIREQTAQGAE 300 ILPRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIREQTAQGAE Sbjct: 241 ILPRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIREQTAQGAE 300 Query: 301 DDALIPGAMARPAKAGSGKRA 321 DDAL+PGAMARPAKAGSGKRA Sbjct: 301 DDALVPGAMARPAKAGSGKRA 321 >gi|300312305|ref|YP_003776397.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 239 bits (609), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 117/290 (40%), Positives = 181/290 (62%) Query: 3 FDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLTPA 62 F+L+DLRLF+ I E S+T A+R +++L + S RI+ +E GT LL R +GVQ T A Sbjct: 5 FELSDLRLFLHIVESGSITGGAQRAHMALASASARIRNMEDALGTPLLERGRRGVQTTDA 64 Query: 63 GHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHPQV 122 G L HHA+ QQ+E L+ ++ QY G+KG+IR+ NT+A+TEF+PE+L +FLARHP Sbjct: 65 GRALAHHARMMAQQMEQLRDELGQYARGLKGHIRLLCNTSAMTEFLPEVLARFLARHPHT 124 Query: 123 NVALEERLNHDILRGIQEGTADIGIVAGPVQGEGLQILNFSTDRLVLATALEHPLADAPS 182 ++ LEE+L++++++ + +G AD+GI+A LQ F D LV + HPLA S Sbjct: 125 DIELEEKLSYEVVQAVIDGVADVGIIADSTDSGPLQTYPFRQDHLVAVLSKRHPLARRKS 184 Query: 183 LPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGIGIL 242 L F + L + +GL S L LS G+RL+ R+++RSF+ +CRM+E VG+ +L Sbjct: 185 LAFAELLGEDFVGLSGDSALQQHLSGHATRAGKRLQYRVRLRSFDGICRMVEHQVGVAVL 244 Query: 243 PRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIR 292 P +AA M + + LSD WS R+ + V+ ++ L R L++ ++ Sbjct: 245 PETAALASTPHMDIRAIPLSDGWSRRQLLICVRGLEGLPGPTRELIQALQ 294 >gi|300311542|ref|YP_003775634.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 335 Score = 92.8 bits (229), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 79/319 (24%), Positives = 141/319 (44%), Gaps = 17/319 (5%) Query: 8 LRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLTPAGHTLL 67 L+ F+ +A+ +A+R ++S A S I+++E G RL R+++ V LTP G + Sbjct: 10 LQAFLALADCRHFGHAAQRCHVSQSAFSAMIQKIEAAAGARLFERDTRNVTLTPEGELFV 69 Query: 68 HHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHPQVNVALE 127 A+Q + +E SDM Y KG + I A + ++P +L + R+P V V L Sbjct: 70 QVARQLVSDIEAAFSDMSDYVARRKGRVAIAALPSLAAGWLPPVLADYRQRYPGVTVELF 129 Query: 128 ERLNHDILRGIQEGTADIGIVAGPVQGEGLQILNFST-----DRLVLATALEHPLADAPS 182 + ++ L ++ G DI + A G +L F T D L +H LA Sbjct: 130 DAISDQCLDLLRHGKVDIALTA-----PGPNLLEFDTRPLCSDPFYLVCRKDHKLASKRR 184 Query: 183 LPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGIGIL 242 + Q E I L +++ L ++ G + ++V + +IE+ +G+ ++ Sbjct: 185 IKVPQLAGCEMIHLARSTSVRQHLDAVLRPGGV-IHTGLEVEHLATVAALIESGLGVSVV 243 Query: 243 PRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIREQTAQGAEDD 302 P + + L + L P R +V +LS A+ L+E + + E + Sbjct: 244 PELTLFQFRLP-NLVAIPLDAPELKRPLLIVTPKERSLSVAAQGLLELVEGKAKAETETE 302 Query: 303 ALIPGAMARPAKAGSGKRA 321 A + G + SG+RA Sbjct: 303 AAVAG-----KRQESGRRA 316 >gi|300309393|ref|YP_003773485.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 82/274 (29%), Positives = 133/274 (48%), Gaps = 22/274 (8%) Query: 3 FDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLTPA 62 +L LR F+ +AE S TR+AE++ + S +IK LE G L R S+GV+L+ Sbjct: 1 MELRHLRYFLAVAEQRSFTRAAEKVGIGQPPLSMQIKALEEEVGGALFLRSSQGVELSEV 60 Query: 63 GHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEI---LGKFLARH 119 G LL HAQ+ + Q E +Q +G G +R+ ++AV F P + L +F RH Sbjct: 61 GRVLLPHAQRAVDQAEQALRAARQAASGETGLLRMGFTSSAV--FHPLVSHALQQFRERH 118 Query: 120 PQVNVALEERLNHDILRGI---QEGTADIGIVAGPVQGEGLQILNFSTDRLVLATALEHP 176 ++ ++L E + +L I Q A + IV P L ++ ++L +A HP Sbjct: 119 RKLEISLSEGITEQLLADIAASQLDAAFVRIVTTP--SAALGVVELPAEKLKVALPRSHP 176 Query: 177 LADAPSLPFIQTLEYEH---IGLHEGSTLHHFLSKIVAENG---QRLKLRIQVRSFEAMC 230 LA SL +Q L E+ + +GS L+ + G Q ++L Q+ S Sbjct: 177 LARRKSL-RLQDLASENFIMVPRGKGSALYDSIFSACQAAGFDPQVIQLAPQLTS---AI 232 Query: 231 RMIETNVGIGILPRSAAQRHQQTMQLSVVELSDP 264 ++ + +GI I+P + Q Q Q+ VE+ +P Sbjct: 233 NLVASGLGISIVPEAIEQ--VQLAQVRYVEIVEP 264 >gi|300311724|ref|YP_003775816.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 297 Score = 85.1 bits (209), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 71/294 (24%), Positives = 137/294 (46%), Gaps = 3/294 (1%) Query: 1 MLFDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLT 60 M D+ ++ F+ IA+H S +A ++++ A + R+++LE G L+ R ++ + LT Sbjct: 1 MKIDILGVQAFVAIADHGSFQGAAGALHVTQTAVTLRLRKLEDFLGVTLIERTTRSIALT 60 Query: 61 PAGHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHP 120 G L A++ + ++ ++++ +G + I T +++P IL + AR+P Sbjct: 61 EIGRDFLPQARRLLAELAGALVEIRETGIARRGDVSIACVPTVGVQYLPRILQAYSARYP 120 Query: 121 QVNVALEERLNHDILRGIQEGTADIGI-VAGPVQGEGLQILNFSTDRLVLATALEHPLAD 179 + + + + + + + GI +AG E + + + DR VL +HPLA Sbjct: 121 HNRIKILDHASSAVAEAVLRREVEFGIHIAGEHHPELISV-ALTEDRYVLICPKDHPLAR 179 Query: 180 APSLPFIQTLEYEHIGLHEGSTLHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGI 239 + + Q Y I E S+ L + NG L+ +V+ ++ VG+ Sbjct: 180 RRRIAWRQLQSYPLIFAGEVSSNRALLDPTLKANGFALRSFYEVQRSSTAVGLVAQGVGV 239 Query: 240 GILPRSAAQRHQQTMQLSVVELSDPWSVRERSMVVQDIDTLSPHARNLVEFIRE 293 ++P+ A Q + V+EL DP R ++ + LSP A+ L + IRE Sbjct: 240 AVVPKLAIQEGAYPT-VRVIELVDPVVSRSLVLITRKAAHLSPAAQALYDMIRE 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 938,562 Number of extensions: 37863 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 68 Length of query: 321 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 236 Effective length of database: 1,217,395 Effective search space: 287305220 Effective search space used: 287305220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)