BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0063.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 810 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 186 4e-48 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 181 7e-47 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 159 4e-40 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 113 2e-26 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 810 bits (2092), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/388 (99%), Positives = 388/388 (100%) Query: 1 MSYPTASDSHAELRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEY 60 MSYPTASDSHA+LRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEY Sbjct: 1 MSYPTASDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEY 60 Query: 61 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRL 120 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRL Sbjct: 61 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRL 120 Query: 121 QSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSR 180 QSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSR Sbjct: 121 QSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSR 180 Query: 181 KSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL 240 KSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL Sbjct: 181 KSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL 240 Query: 241 DGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAAN 300 DGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAAN Sbjct: 241 DGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAAN 300 Query: 301 LMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRET 360 LMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRET Sbjct: 301 LMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRET 360 Query: 361 RLYQVAPISTNLIFSYVAEHVLGLPRSF 388 RLYQVAPISTNLIFSYVAEHVLGLPRSF Sbjct: 361 RLYQVAPISTNLIFSYVAEHVLGLPRSF 388 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 186 bits (471), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 122/373 (32%), Positives = 193/373 (51%), Gaps = 31/373 (8%) Query: 7 SDSHAELRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLG 66 S H +RDA+R + + D +P + + L + G + +P+++GG+G+ Sbjct: 4 SPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMD 63 Query: 67 LTEASVIMEEINRTGG---------NSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGE 117 + +EEI G NS AC G ++GSP QKE++L +A GE Sbjct: 64 YMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPLARGE 116 Query: 118 LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQ 177 + L +TEP TG+D I+T A + GD +++NG K +I+ +H+ + I+ A T Sbjct: 117 M-LGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVT----D 171 Query: 178 VSRKSEGMSIFLVDLKE---AIGHGMEVRPILNMVNH--ETNELFFDNLEIPAENLIGEE 232 S +G+S FLV +G E M H +T ++ DN +PA L+G+E Sbjct: 172 RSAGKKGISCFLVPCDTPGFVVGRSEE-----KMGQHASDTVQIMLDNCRVPATALLGKE 226 Query: 233 GRGFRYILDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEA 292 G G+R L L A R IAA+ +G + A YAR+R F PI +Q V F LA+ Sbjct: 227 GEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADM 286 Query: 293 YIEVEAANLMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYD 352 ++AA LM ++A L D + C +A+MAK A++AA + A+ +Q HGG G+ ++ Sbjct: 287 NTLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFP 346 Query: 353 VERKFRETRLYQV 365 VER +R+ R+ Q+ Sbjct: 347 VERIYRDVRICQI 359 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 181 bits (460), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 127/383 (33%), Positives = 197/383 (51%), Gaps = 21/383 (5%) Query: 4 PTASDSHAE----LRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQE 59 P S H E LR+AV + +ID + +P L G L + +E Sbjct: 5 PGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEE 64 Query: 60 YGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNM--GTLLRHGSPEQKERYLPRIASGE 117 YGG+GLG + MEEI+R + G +G N+ + R+G+ EQK +YLPR+ SG+ Sbjct: 65 YGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGD 124 Query: 118 LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQ 177 + ++A++EP G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T LE Sbjct: 125 F-IGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTD-LEA 182 Query: 178 VSRKSEGMSIFLVDLKEAIGHGMEVR-PILNMVNHETNELFFDNLEIPAENLIGEEGRGF 236 +R GM+ FLV+ K G + + L M T EL F + E+P EN++G GRG Sbjct: 183 GAR---GMTAFLVE-KGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGV 238 Query: 237 RYILDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEV 296 ++ GL+ ER++++ +G +D Y +R F + IG Q +Q LA+ Y + Sbjct: 239 NVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTM 298 Query: 297 EAANLMRY---QACALFDAQQSCGA----QANMAKYLAAKAAWEAANVCLQTHGGFGFAC 349 A Y QAC D+ A A Y A KA W A +Q+ GG G+ Sbjct: 299 MACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGE-AIQSLGGNGYIN 357 Query: 350 EYDVERKFRETRLYQVAPISTNL 372 EY V R +R+ +LY++ ++ + Sbjct: 358 EYPVGRLWRDAKLYEIGAGTSEI 380 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 159 bits (401), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 109/374 (29%), Positives = 188/374 (50%), Gaps = 12/374 (3%) Query: 13 LRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASV 72 + + RD + K DE +P + + G+ P+ GG GL +A+V Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 73 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSPEQKERYLPRIASGELRLQSMAVTEPTT 130 + EE+ R S A + ++NM + + G P K ++ ++A+G ++ S +TEP + Sbjct: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGR-KIGSYCLTEPGS 129 Query: 131 GTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLV 190 G+D +KT A + YV+NG K +IS +D+++++ART + G+S +V Sbjct: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 Query: 191 DLKE-AIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTL 249 + +G + + + T + FDN+++PA++L+G+EG+GF + + GL+ R Sbjct: 185 PGNAPGVSYGKK-ESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRIN 243 Query: 250 IAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAA-NLMRYQACA 308 IA +G +D A Y +ER F RPI Q +QF LA+ E+ AA ++R A Sbjct: 244 IATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATK 303 Query: 309 LFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVAPI 368 L MAK LA + N LQ HGG+G+ EY +ER FR+ R++Q+ Sbjct: 304 LDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEG 363 Query: 369 STNLIFSYVAEHVL 382 + ++ ++ +L Sbjct: 364 TNEIMRVIISRKLL 377 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 86/317 (27%), Positives = 146/317 (46%), Gaps = 32/317 (10%) Query: 15 DAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIM 74 D +R + T P K DE F A+ AG++ AL+P+ +GG+ L + ++ Sbjct: 24 DVLRKVGPTITP--LVKPDERFYATRPFYQAMADAGFIKALLPESFGGTALSTLDFALAA 81 Query: 75 EEINRTGGN-SGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVTEPTTGTD 133 EE+ N A G + ++ HG+PEQK+R+LP RL ++A TE T G + Sbjct: 82 EELTAVDVNVPSALLGTGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGAN 141 Query: 134 ------TTRIKTVAVKKGDRYVINGQKVWISRVQHSD-----LMILLARTTP-------L 175 + ++T A +GD +VING K + + D L ++ RT P L Sbjct: 142 YDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSL 201 Query: 176 EQVSRKSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRG 235 + I +V L + +GH + P + F+N+ +PA N++G+ G G Sbjct: 202 AVIMVPGNAPGIEIVGLLDTMGHRATISP----------RVHFNNVRVPASNIVGKPGDG 251 Query: 236 FRYILDGLNAERTLIAAECIGDGYWFIDKATQYAR-ERVVFDRPIGMNQGVQFPLAEAYI 294 + + LI + C+G D A ++A+ +R P+ +Q V F LA+ + Sbjct: 252 ISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPVIEHQNVGFMLADIKM 311 Query: 295 EVEAANLMRYQACALFD 311 +EAA + +++C D Sbjct: 312 RIEAARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,187,008 Number of extensions: 48868 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 9 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)