BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0060.p (374 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 746 0.0 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 224 6e-60 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 136 3e-33 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 87 2e-18 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 84 2e-17 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 746 bits (1926), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/374 (100%), Positives = 374/374 (100%) Query: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 61 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN Sbjct: 61 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 Query: 121 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT 180 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT Sbjct: 121 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT 180 Query: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGAT 240 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGAT Sbjct: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGAT 240 Query: 241 ATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHW 300 ATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHW Sbjct: 241 ATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHW 300 Query: 301 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFD 360 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFD Sbjct: 301 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFD 360 Query: 361 RLHGGEGLRDLIVF 374 RLHGGEGLRDLIVF Sbjct: 361 RLHGGEGLRDLIVF 374 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 224 bits (572), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 135/377 (35%), Positives = 203/377 (53%), Gaps = 12/377 (3%) Query: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 MK +AAV + +PL IEE++L P GEVLV +KA G+CH+D ++G P Sbjct: 1 MKTRAAV--------AWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP 52 Query: 61 RPV-PMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 + P LGHE + VV+VG GV L+ D V+ ++ P C C C+ + LC+ AT Sbjct: 53 EGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRAT 112 Query: 120 NTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 G + R +P+ H++G S F+++ VV + KI +D + GC V Sbjct: 113 QGRGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVT 172 Query: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGA 239 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ ++ A+AR G Sbjct: 173 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGM 232 Query: 240 TATVNARD-PDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPG 297 T +N +D P+ V+ + +T GG DY+FE G+ M + +G G ++ G+ A G Sbjct: 233 TDFINPKDVPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAG 292 Query: 298 QHWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINR 357 Q + + LV R KGS G D+P+ + + GKL +D L+ +L L IN Sbjct: 293 QEISTRPFQLVT-GRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINE 351 Query: 358 GFDRLHGGEGLRDLIVF 374 GFD + GE +R ++ F Sbjct: 352 GFDLMKSGESIRSVVEF 368 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 136 bits (342), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 117/368 (31%), Positives = 183/368 (49%), Gaps = 45/368 (12%) Query: 3 IQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITG---VR 59 ++AAV+RE +PL IE++ + P PG++LV+ +A+G+CH+DL G V+ Sbjct: 5 MKAAVVREFG--------KPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVK 56 Query: 60 PRPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFV-PSCGHCLPCMEGRPALCEPGAA 118 P P P GHE + V VG+GV ++ GD V + ++ +CG C PC G LC A Sbjct: 57 PTP-PFIPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLC---AE 112 Query: 119 TNTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAV 178 G ++G +FA++ + + + ++D AA CA Sbjct: 113 QQNTGYSVNG------------------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAG 154 Query: 179 LTGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLG 238 +T V + EV+ G + G+GG+G A+ A A G V+A D+H+ KLALA+ LG Sbjct: 155 VT-VYKGLKETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLG 212 Query: 239 ATATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQ 298 A TV+ R+ +AV +V+ + GG A A S +AME A+ R G GLP PG Sbjct: 213 ADLTVDGRNHNAVAEVQRII-GGAHGALVTAVSPKAMEQAFGFLRARGTMALVGLP-PGD 270 Query: 299 HWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRG 358 +L + V + TV+GS +G+ +D+ + GK+ + L +IN Sbjct: 271 -ISLPVFNTVLKRITVRGSIVGT---RQDLEESLVFAAEGKVAAHFTWDK---LDNINAI 323 Query: 359 FDRLHGGE 366 F R+ G+ Sbjct: 324 FARMEEGK 331 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 101/354 (28%), Positives = 143/354 (40%), Gaps = 42/354 (11%) Query: 37 EVLVRMKAAGLCHSDLSVITGVRP-RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVF 95 +V++++ A +C SDL + G P LGHE VVE G + L+ GD VV+ F Sbjct: 27 DVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVVVPF 86 Query: 96 VPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVS 155 V +CG C C + A CE TN L + +R G S Sbjct: 87 VIACGQCFFCDQHLYAACE---TTNPDRGALMNAKGIRSGAA----MFGYSHLYGGMPGG 139 Query: 156 RRSCVKI-EADIDPV--------QAALFGCAVLTGVGAAVNTAEVKAGTTAAVLGLGGVG 206 + V++ +AD+ P+ + LF +L AV A V+ G++ A+ G G VG Sbjct: 140 QAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVKNAGVREGSSVAIYGAGPVG 199 Query: 207 LCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVRALTRG-GVD-- 263 A A GA + VD H +L A+ VN D E + A T G GVD Sbjct: 200 QMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQVDPAEYIIANTAGRGVDGV 259 Query: 264 ---YAFEMAGSV---------------QAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQV 305 FE GSV QA+ RRGG+ G+ A H L Sbjct: 260 IDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPGVYAGFIHGFLFG- 318 Query: 306 SLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGF 359 + + TVK G +P + + G+L D ++ RL L D RG+ Sbjct: 319 DIFEKGVTVK---TGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGY 369 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 77/275 (28%), Positives = 114/275 (41%), Gaps = 22/275 (8%) Query: 23 LKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVI----TGVRPRPVPMALGHEASAEVVEV 78 L+I+ LD P ++ V++KA G+C SDL GV PM LGHE S ++ EV Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 79 GSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDKTQP 138 G+ V D+ G + + CG C C EG+ C R Sbjct: 73 GTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCL--------------DMRFYGSAMR 118 Query: 139 VHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGTTAA 198 H G AF ++ R + + +AA+ L+ AV A G Sbjct: 119 TPHVQG--AFRQEIIIERSQAHVVADHVSDGEAAM--AEPLSVALHAVRRAGPLVGKRVL 174 Query: 199 VLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVRALT 258 V G G +G ++ A +GA EV+ D+ L A +GA + +N + A K A Sbjct: 175 VTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAAD 234 Query: 259 RGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGL 293 +G D FE +G+ +A+ A R + V GL Sbjct: 235 KGTFDVLFEASGNERALVGALEALRPQAIIVQVGL 269 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,147,817 Number of extensions: 48992 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 7 Length of query: 374 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 287 Effective length of database: 1,207,925 Effective search space: 346674475 Effective search space used: 346674475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)