BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0051.p (513 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspi... 1054 0.0 gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herba... 75 1e-14 >gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 513 Score = 1054 bits (2725), Expect = 0.0, Method: Compositional matrix adjust. Identities = 512/513 (99%), Positives = 513/513 (100%) Query: 1 MTTQNTPAAQPAADENSIIAERRAKLAAIREQGVAFPNDFRPANQAADLHAKYDGFENEA 60 MTTQNTPAAQPAADENSIIAERRAKLAAIREQGVAFPNDFRPANQAADLHAKYDGFENEA Sbjct: 1 MTTQNTPAAQPAADENSIIAERRAKLAAIREQGVAFPNDFRPANQAADLHAKYDGFENEA 60 Query: 61 LEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLYVTRDVTGEEAMAAFKHY 120 LEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLY+TRDVTGEEAMAAFKHY Sbjct: 61 LEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLYITRDVTGEEAMAAFKHY 120 Query: 121 DLGDILGAEGTLFKTKTGELSVKVTKLKLITKSLRPLPDKFHGLADQEMKYRQRYVDLIM 180 DLGDILGAEGTLFKTKTGELSVKVTKLKLITKSLRPLPDKFHGLADQEMKYRQRYVDLIM Sbjct: 121 DLGDILGAEGTLFKTKTGELSVKVTKLKLITKSLRPLPDKFHGLADQEMKYRQRYVDLIM 180 Query: 181 SEETRRTFKARTAAMSSIRRFMETHGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFM 240 SEETRRTFKARTAAMSSIRRFMETHGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFM Sbjct: 181 SEETRRTFKARTAAMSSIRRFMETHGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFM 240 Query: 241 RIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYVDYQWLMGFTEQ 300 RIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYVDYQWLMGFTEQ Sbjct: 241 RIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYVDYQWLMGFTEQ 300 Query: 301 VIRQAAIDAHGTAVLSYQGRELDLSKPFDRLTITGAILKYAPNYTEAQLADIDFLRTELK 360 VIRQAAIDAHGTAVLSYQGRELDLSKPFDRLTITGAILKYAPNYTEAQLADIDFLRTELK Sbjct: 301 VIRQAAIDAHGTAVLSYQGRELDLSKPFDRLTITGAILKYAPNYTEAQLADIDFLRTELK 360 Query: 361 KFGVKTDQAPLAGAGIGALQLALFEETAESQLWNPTYIIDYPVEVSPLARASDTVAGITE 420 KFGVKTDQAPLAGAGIGALQLALFEETAESQLWNPTYIIDYPVEVSPLARASDTVAGITE Sbjct: 361 KFGVKTDQAPLAGAGIGALQLALFEETAESQLWNPTYIIDYPVEVSPLARASDTVAGITE 420 Query: 421 RFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGDEEAMYYDADYIRALEYGMPPA 480 RFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGDEEAMYYDADYIRALEYGMPPA Sbjct: 421 RFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGDEEAMYYDADYIRALEYGMPPA 480 Query: 481 GGCGIGIDRLMMLITDSPNIRDVILFPHLRRED 513 GGCGIGIDRLMMLITDSPNIRDVILFPHLRRED Sbjct: 481 GGCGIGIDRLMMLITDSPNIRDVILFPHLRRED 513 >gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 599 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 71/303 (23%), Positives = 133/303 (43%), Gaps = 27/303 (8%) Query: 49 LHAKYDGFENEALEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLYVTRD- 107 + +Y G EAL + TV++ G + +R G F L+D G+ +Q+ D Sbjct: 1 MRTQYCGLTTEALLGQ--TVSLCGWVHRRRDHGGVIFIDLRDREGL-----VQVVCDPDR 53 Query: 108 ----VTGEEAMAAFKHYDLGDILG-AEGTLFKT-KTGELSVKVTKLKLITKSLRPLPDKF 161 E F G + EGT K+G++ V +L+++ S+ P Sbjct: 54 ADVFKNAESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLD 113 Query: 162 HGLADQEMKYRQRYVDLIMSEETRRTFKARTAAMSSIRRFMETHGFMEVETPML-HPIPG 220 + + R +DL + + + R +R+F++ GF+++ETPML P Sbjct: 114 DDSLSETTRLTHRVLDL-RRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPE 172 Query: 221 GAAAKPFITHHNALDMQMFMRIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFT 280 GA + NA + + +P+L+ + L+V F+R ++I + FR+E + PEFT Sbjct: 173 GARDYLVPSRVNAGEF-FALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFT 231 Query: 281 MMEFYAAYVDYQWLMGFTEQVIRQAAIDAHGTAVLSYQGRELDLSKPFDRLTITGAILKY 340 ++ ++++ Q + E +IR ++ ++DL PF + A+ KY Sbjct: 232 QIDCETSFLNEQEIRDLFEGMIR----------LVFKNALDIDLPNPFPVMDFATAMGKY 281 Query: 341 APN 343 + Sbjct: 282 GSD 284 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 58/212 (27%), Positives = 90/212 (42%), Gaps = 50/212 (23%) Query: 332 TITGAILKYAPNYTEAQLADIDFLRTELKKFGVKTDQAPLAGAGIGALQL---------- 381 I A L T AQ D+ F FG D+A + IGAL++ Sbjct: 373 NIHDAALNAILEKTGAQDGDLIF-------FG--ADKAKVVNDAIGALRVKIGHSEFGKK 423 Query: 382 -ALFEETAESQLWNPTYIIDYPVE----------------VSPLARASDTV-----AGIT 419 LF++ W P +++D+P+ SP D + A I Sbjct: 424 NGLFDDA-----WRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIA 478 Query: 420 ERFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGDEEAMYYDADYIRALEYGMPP 479 + +++ + G E+ G ++ A+ Q+ F A DEEA + AL+YG PP Sbjct: 479 KAYDMVLNGWELGGGSVRIHRADVQSKVFRAL----KIDDEEAKLKFGFLLDALQYGAPP 534 Query: 480 AGGCGIGIDRLMMLITDSPNIRDVILFPHLRR 511 GG G+DRL+ ++ + +IRDVI FP +R Sbjct: 535 HGGIAFGLDRLVTMMAGAESIRDVIAFPKTQR 566 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,466,099 Number of extensions: 58966 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 159 Number of HSP's successfully gapped: 3 Length of query: 513 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 424 Effective length of database: 1,198,455 Effective search space: 508144920 Effective search space used: 508144920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)